Team:Johns Hopkins/Notebook/GROUP 7: Microscopy/Yeast

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Latest revision as of 03:19, 30 October 2008

GROUP 7: Microscopy/Yeast

 Milestones 
 * GFP-expressing yeast have been visualized by microscopy and photographed. The pictures of these can
   be found on our poster.
 * We have the resources to photograph green, various red, yellow, blue, and violet fluorophores. 
 * STE3-GFP "sex detector" has been transformed into MATa, MATalpha, and MATa/alpha. Permanent cultures
   of these will be banked in the Boeke lab early in the week of November 3rd.
 * For micrographs of STE3-GFP "sex detector" cells/colonies, see the poster.

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    Milestones
-   Have gfp yeast been visualized by microscopy?
+   * GFP-expressing yeast have been visualized by microscopy and photographed. The pictures of these can
-  Have gfp yeast been photographed? Link to moodle – picture.
+    be found on our poster.
-  [If pics are nice, put on our web page]
+   * We have the resources to photograph green, various red, yellow, blue, and violet fluorophores.
-   Can green(/other colors) colonies be photographed?
+   * STE3-GFP "sex detector" has been transformed into MATa, MATalpha, and MATa/alpha. Permanent cultures
-   Has STE3-gfp sex detector been transformed into MATa, MATalpha, and MATa/alpha?  Y/N
+    of these will be banked in the Boeke lab early in the week of November 3rd.
-  Have permanent cultures been banked in the Boeke lab? Y/N
+   * For micrographs of STE3-GFP "sex detector" cells/colonies, see the poster.
-   Have STE3-gfp sex detector cells/colonies been photographed Y/N  - link to Moodle