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Constructs tested
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| - | + | {| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center" | |
| - | + | !align="center"|[[Team:Davidson-Missouri_Western|Home]] | |
| - | + | !align="center"|[[Team:Davidson-Missouri_Western/Team|The Team]] | |
| - | + | !align="center"|[[Team:Davidson-Missouri_Western/Project|''E. nigma'' Project]] | |
| - | + | !align="center"|[[Team:Davidson-Missouri_Western/New_Parts_Contributed_to_the_Registry|Parts Submitted to the Registry]] | |
| - | + | !align="center"|[[Team:Davidson-Missouri_Western/Notebook|Notebook]] | |
| + | |} | ||
| + | <br> | ||
| + | |||
| + | |||
| + | '''Experiment to test the function of old and new Lac promoters''' | ||
| + | |||
| + | <br> | ||
| + | Each of the promoters is used to drive expression of GFP so that fluorescence can be used to measure promoter function. The data demonstrate that the new Lac promoters have improved function over the existing wild type promoter in the Registry. | ||
| + | <br> | ||
| + | <br> | ||
| + | [[Image:Trial1.png]] | ||
| + | </center> | ||
| + | <br> | ||
| + | <br> | ||
| + | '''Experiments to test basal function of XOR gate constructs''' | ||
| + | <br> | ||
| + | <br> | ||
| + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K091173 '''K091173'''] was coplated with the K09100 Lux receiver. Green fluorescence indicates that at least one of the two hybrid promoters in the XOR construct is driving expression of the LuxI gene. | ||
| + | <br> | ||
| + | <center> | ||
| + | [[Image:9100_K82.JPG|389px]] | ||
| + | </center> | ||
| + | <br> | ||
| + | |||
| + | XOR gate construct [http://partsregistry.org/wiki/index.php?title=Part:BBa_K091182 '''K091182'''] | ||
| + | coplated with the K09100 Lux receiver. Green fluorescence indicates that at least one of the two hybrid promoters in the XOR construct is driving expression of the LuxI gene. | ||
| + | <center> | ||
| + | [[Image:9100_K73.JPG|389px]] | ||
| + | </center> | ||
| + | <br> | ||
Latest revision as of 03:22, 30 October 2008
| Home | The Team | E. nigma Project | Parts Submitted to the Registry | Notebook |
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Experiment to test the function of old and new Lac promoters
Each of the promoters is used to drive expression of GFP so that fluorescence can be used to measure promoter function. The data demonstrate that the new Lac promoters have improved function over the existing wild type promoter in the Registry.
</center>
Experiments to test basal function of XOR gate constructs
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K091173 K091173] was coplated with the K09100 Lux receiver. Green fluorescence indicates that at least one of the two hybrid promoters in the XOR construct is driving expression of the LuxI gene.
XOR gate construct [http://partsregistry.org/wiki/index.php?title=Part:BBa_K091182 K091182] coplated with the K09100 Lux receiver. Green fluorescence indicates that at least one of the two hybrid promoters in the XOR construct is driving expression of the LuxI gene.
