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| {{Imperial/StartPage2}} | | {{Imperial/StartPage2}} |
- | === BioBricks & Characterisation === | + | |
| + | === BioBricks Submitted === |
| {{Imperial/Box2|| | | {{Imperial/Box2|| |
- | Welcome to our Biobricks and Characterisation page! Here you can find information about the parts, devices and systems we have produced.
| + | This year Imperial College has added 45 new parts to the Registry - 45 of those designed specifically for use in ''B. subtilis''. In addition we sought to characterise some of these parts, including the constitutive promoter and ribosome binding site '''pveg-spoVG'''; you can find information about the characterisation of some of our parts in the Major Results page. |
| | | |
- | Shown below is our final construct - our light-sensing, clutch-engaging, biomaterial-producing biofabricator. If you move your mouse over a part, it will light up and you can click it to visit its page in the iGEM Registry. If you select the name of a device (or the whole system) underneath the diagram, that device will show up and clicking the name will take you to its charaterisation page. | + | Shown below is the final construct we were aiming for - our light-sensing, clutch-engaging, biomaterial-producing biofabricator. |
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- | <html><center><img width="800px" src="http://i59.photobucket.com/albums/g305/Timpski/S1L.png"> | + | <html><center><img width="800px" src="http://i59.photobucket.com/albums/g305/Timpski/S1L.png"></center></html> |
- | <br>| Device 1 | Device 2 | Device 3 |<br>| The System |</center></html>
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| }} | | }} |
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- | {{Imperial/Box1|Parts Submitted to the Registry|For now, here are the parts we will submit to the Registry. They can also be accessed <html><a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2008&group=Imperial_College">here</a>. | + | {{Imperial/Box1|BioBricks Submitted to the Registry| |
- | <br><br>
| + | The parts we have submitted to the Registry are shown in the table below; they can also be accessed <html><a target="_blank" href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2008&group=Imperial_College">here</a>. |
- | <html><iframe src="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2008&group=Imperial_College" width="100%" height="600px" scrolling="yes" clip="true"></iframe></html> | + | <br> |
| + | <html><iframe src="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2008&group=Imperial_College" width="100%" height="600px"></iframe></html> |
| }} | | }} |
| | | |
- | {{Imperial/Box1|Production Status| | + | {{Imperial/EndPage|Protocols|Major_Results}} |
- | This table shows our progress with each part:}}
| + | |
- | | + | |
- | {| width="100%" style="background-color:transparent;"
| + | |
- | |
| + | |
- | {| border="2" cellpadding="2" style="text-align:center" align="right" width="70%"
| + | |
- | ! Key
| + | |
- | |- style=background:gold
| + | |
- | | Submitted
| + | |
- | |- style=background:silver
| + | |
- | | Sequenced
| + | |
- | |- style=background:#cc4400
| + | |
- | | Midiprepped
| + | |
- | |- style=background:lawngreen
| + | |
- | | Fully verified
| + | |
- | |- style=background:mediumslateblue
| + | |
- | | Partially Verified
| + | |
- | |- style=background:royalblue
| + | |
- | | Transformed
| + | |
- | |- style=background:yellow
| + | |
- | | Ligation pending/Failed
| + | |
- | |- style=background:red
| + | |
- | | Awaiting GeneArt
| + | |
- | |- style=background:maroon
| + | |
- | | Cloning difficulties
| + | |
- | |}
| + | |
- | |
| + | |
- | {| border="2" cellpadding="2" style="text-align:left" align="left" width="90%"
| + | |
- | !Part Number !! Part Name !! Status
| + | |
- | |- style=background:silver
| + | |
- | | K143001 || 5' AmyE Integration Sequence || Sequenced
| + | |
- | |- style=background:silver
| + | |
- | | K143002 || 3' AmyE Integration Sequence || Sequenced
| + | |
- | |- style=background:mediumslateblue
| + | |
- | | K143005 || 5' EpsE Integration Sequence || PCR Verified
| + | |
- | |- style=background:#cc4400
| + | |
- | | K143006 || 3' EpsE Integration Sequence || Sent for Sequencing
| + | |
- | |- style=background:yellow
| + | |
- | | K143008 || 5' PyrD Integration Sequence || Pending
| + | |
- | |- style=background:mediumslateblue
| + | |
- | | K143009 || 3' PyrD Integration Sequence || PCR verified
| + | |
- | |- style=background:#cc4400
| + | |
- | | K143031 || Aad9 Spectinomycin Resistance Gene || Sent for Sequencing
| + | |
- | |- style=background:silver
| + | |
- | | K143032 || EpsE Molecular Clutch Gene of ''B. subtilis'' || Sequenced
| + | |
- | |- style=background:silver
| + | |
- | | K143033 || LacI (Lva<sup>-</sup>, N terminal deletion) || Sequenced
| + | |
- | |- style=background:#cc4400
| + | |
- | | K143034 || LipA-EAK16 Fusion || Partially Sequenced
| + | |
- | |- style=background:royalblue
| + | |
- | | K143035 || LipA-Human Elastin Fusion || Transformed
| + | |
- | |- style=background:mediumslateblue
| + | |
- | | K143036 || Xylose Operon Regulatory Protein || PCR verified
| + | |
- | |- style=background:mediumslateblue
| + | |
- | | K143037 || Ytva Blue Light Receptor of ''B. subtilis'' || PCR verified
| + | |
- | |- style=background:silver
| + | |
- | | K143038 || SacB-EAK16 Fusion || Sequenced
| + | |
- | |- style=background:maroon
| + | |
- | | K143039 || SacB-Human Elastin Fusion || Fails to clone
| + | |
- | |- style=background:#cc4400
| + | |
- | | K143050 || P43-gsiB || Sent for Sequencing
| + | |
- | |- style=background:silver
| + | |
- | | K143051 || P43-spoVG || Sequenced
| + | |
- | |- style=background:silver
| + | |
- | | K143052 || Pveg-gsiB || Sequenced
| + | |
- | |- style=background:silver
| + | |
- | | K143053 || Pveg-spoVG || Sequenced
| + | |
- | |- style=background:yellow
| + | |
- | | K143054 || Phyperspank-gsiB || Pending
| + | |
- | |- style=background:mediumslateblue
| + | |
- | | K143055 || Phyperspank-spoVG || PCR verified
| + | |
- | |- style=background:yellow
| + | |
- | | K143056 || Pxyl-gsiB || Pending
| + | |
- | |- style=background:yellow
| + | |
- | | K143057 || Pxyl-spoVG || Pending
| + | |
- | |- style=background:#cc4400
| + | |
- | | K143058 || Pctc-gsiB || Midiprepped
| + | |
- | |- style=background:#cc4400
| + | |
- | | K143059 || Pctc-spoVG || Midiprepped
| + | |
- | |- style=background:red
| + | |
- | | K143060 || PgsiB-gsiB || Wrong insert cloned
| + | |
- | |- style=background:silver
| + | |
- | | K143061 || PgsiB-spoVG || Sequenced
| + | |
- | |- style=background:yellow
| + | |
- | | K143062 || LacI - Terminator || Pending
| + | |
- | |- style=background:yellow
| + | |
- | | K143063 || XylR - Terminator || Pending
| + | |
- | |- style=background:#cc4400
| + | |
- | | K143064 || CAT - Terminator || Sent for Sequencing
| + | |
- | |- style=background:yellow
| + | |
- | | K143065 || Aad9 - Terminator || Pending
| + | |
- | |}
| + | |
- | |}
| + | |
- | | + | |
- | <br clear="all"><html><center><table style="color:#ffffff;background-color:#66aadd;" cellpadding="3" cellspacing="1" border="0" bordercolor="#ffffff" align="center">
| + | |
- | <tr><td><ul id="sddm"></html>[[Team:Imperial_College/Major_Results|< Previous]]<html></ul>
| + | |
- | </td><td><ul id="sddm"><a href="#">Back to top</a></ul>
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- | </td><td><ul id="sddm"></html>Next ><html></ul>
| + | |
- | </td></tr></table>
| + | |
- | </center></html>
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- | </div></td></tr></table>
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