Team:Imperial College/BioBricks

From 2008.igem.org

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{{Imperial/StartPage2}}
{{Imperial/StartPage2}}
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=== BioBricks & Characterisation ===
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=== BioBricks Submitted ===
{{Imperial/Box2||
{{Imperial/Box2||
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Welcome to our Biobricks and Characterisation page! Here you can find information about the parts, devices and systems we have produced.
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This year Imperial College has added 45 new parts to the Registry - 45 of those designed specifically for use in ''B. subtilis''. In addition we sought to characterise some of these parts, including the constitutive promoter and ribosome binding site '''pveg-spoVG'''; you can find information about the characterisation of some of our parts in the Major Results page.
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Shown below is our final construct - our light-sensing, clutch-engaging, biomaterial-producing biofabricator. If you move your mouse over a part, it will light up and you can click it to visit its page in the iGEM Registry. If you select the name of a device (or the whole system) underneath the diagram, that device will show up and clicking the name will take you to its charaterisation page.
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Shown below is the final construct we were aiming for - our light-sensing, clutch-engaging, biomaterial-producing biofabricator.
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<html><center><img width="800px" src="http://i59.photobucket.com/albums/g305/Timpski/S1L.png">
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<html><center><img width="800px" src="http://i59.photobucket.com/albums/g305/Timpski/S1L.png"></center></html>
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<br>| Device 1 | Device 2 | Device 3 |<br>| The System |</center></html>
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{{Imperial/Box1|Parts Submitted to the Registry|For now, here are the parts we will submit to the Registry. They can also be accessed <html><a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2008&group=Imperial_College">here</a>.
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{{Imperial/Box1|BioBricks Submitted to the Registry|
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<br><br>
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The parts we have submitted to the Registry are shown in the table below; they can also be accessed <html><a target="_blank" href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2008&group=Imperial_College">here</a>.
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<br>
<html><iframe src="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2008&group=Imperial_College" width="100%" height="600px"></iframe></html>
<html><iframe src="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2008&group=Imperial_College" width="100%" height="600px"></iframe></html>
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{{Imperial/Box1|Production Status|
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{{Imperial/EndPage|Protocols|Major_Results}}
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This table shows our progress with each part:}}
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{| width="100%" style="background-color:transparent;"
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|
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{| border="2" cellpadding="2" style="text-align:center" align="right" width="70%"
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! Key
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|- style=background:gold
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| Submitted
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|- style=background:silver
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| Sequenced
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|- style=background:#cc4400
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| Midiprepped
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|- style=background:lawngreen
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| Fully verified
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|- style=background:mediumslateblue
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| Partially Verified
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|- style=background:royalblue
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| Transformed
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|- style=background:yellow
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| Ligation pending/Failed
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|- style=background:red
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| Awaiting GeneArt
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|- style=background:maroon
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| Cloning difficulties
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|}
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|
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{| border="2" cellpadding="2" style="text-align:left" align="left" width="90%"
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!Part Number !! Part Name !! Status
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|- style=background:silver
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| K143001 || 5' AmyE Integration Sequence || Sequenced
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|- style=background:silver
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| K143002 || 3' AmyE Integration Sequence || Sequenced
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|- style=background:mediumslateblue
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| K143005 || 5' EpsE Integration Sequence || PCR Verified
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|- style=background:#cc4400
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| K143006 || 3' EpsE Integration Sequence || Sent for Sequencing
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|- style=background:yellow
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| K143008 || 5' PyrD Integration Sequence || Pending
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|- style=background:mediumslateblue
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| K143009 || 3' PyrD Integration Sequence || PCR verified
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|- style=background:#cc4400
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| K143031 || Aad9 Spectinomycin Resistance Gene || Sent for Sequencing
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|- style=background:silver
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| K143032 || EpsE Molecular Clutch Gene of ''B. subtilis'' || Sequenced
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|- style=background:silver
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| K143033 || LacI (Lva<sup>-</sup>, N terminal deletion) || Sequenced
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|- style=background:#cc4400
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| K143034 || LipA-EAK16 Fusion || Partially Sequenced
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|- style=background:royalblue
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| K143035 || LipA-Human Elastin Fusion || Transformed
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|- style=background:mediumslateblue
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| K143036 || Xylose Operon Regulatory Protein || PCR verified
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|- style=background:mediumslateblue
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| K143037 || Ytva Blue Light Receptor of ''B. subtilis'' || PCR verified
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|- style=background:silver
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| K143038 || SacB-EAK16 Fusion || Sequenced
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|- style=background:maroon
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| K143039 || SacB-Human Elastin Fusion || Fails to clone
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|- style=background:#cc4400
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| K143050 || P43-gsiB || Sent for Sequencing
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|- style=background:silver
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| K143051 || P43-spoVG || Sequenced
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|- style=background:silver
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| K143052 || Pveg-gsiB || Sequenced
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|- style=background:silver
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| K143053 || Pveg-spoVG || Sequenced
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|- style=background:yellow
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| K143054 || Phyperspank-gsiB || Pending
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|- style=background:mediumslateblue
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| K143055 || Phyperspank-spoVG || PCR verified
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|- style=background:yellow
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| K143056 || Pxyl-gsiB || Pending
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|- style=background:yellow
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| K143057 || Pxyl-spoVG || Pending
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|- style=background:#cc4400
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| K143058 || Pctc-gsiB || Midiprepped
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|- style=background:#cc4400
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| K143059 || Pctc-spoVG || Midiprepped
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|- style=background:red
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| K143060 || PgsiB-gsiB || Wrong insert cloned
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|- style=background:silver
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| K143061 || PgsiB-spoVG || Sequenced
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|- style=background:yellow
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| K143062 || LacI - Terminator || Pending
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|- style=background:yellow
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| K143063 || XylR - Terminator || Pending
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|- style=background:#cc4400
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| K143064 || CAT - Terminator || Sent for Sequencing
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|- style=background:yellow
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| K143065 || Aad9 - Terminator || Pending
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|}
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|}
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<br clear="all"><html><center><table style="color:#ffffff;background-color:#66aadd;" cellpadding="3" cellspacing="1" border="0" bordercolor="#ffffff" align="center">
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Latest revision as of 03:25, 30 October 2008


BioBricks Submitted

This year Imperial College has added 45 new parts to the Registry - 45 of those designed specifically for use in B. subtilis. In addition we sought to characterise some of these parts, including the constitutive promoter and ribosome binding site pveg-spoVG; you can find information about the characterisation of some of our parts in the Major Results page.

Shown below is the final construct we were aiming for - our light-sensing, clutch-engaging, biomaterial-producing biofabricator.


BioBricks Submitted to the Registry

The parts we have submitted to the Registry are shown in the table below; they can also be accessed here.



Retrieved from "http://2008.igem.org/Team:Imperial_College/BioBricks"