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Team:Paris/Modeling/Protocol Of Characterisation
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The previous experiment is used to find parameters regarding to our modelization. However, the experiments themselves must be described in the same way to involve those parameters in a consistent way, and actually, to be interpreted. | The previous experiment is used to find parameters regarding to our modelization. However, the experiments themselves must be described in the same way to involve those parameters in a consistent way, and actually, to be interpreted. | ||
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Latest revision as of 03:39, 30 October 2008
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Protocol of Characterization
Other pages:
Principles of the ExperimentsTo evaluate quantitativly the activity of a promoter in function of its transcription factors, we need datas in which the different values of the activities are correlated with various known and controlled values of the transcription factors concentrations. Therefore, we designed a generic plasmid in which the transcription factors are put under the control of previously characterized inducible promoter, and the studied promoter is put before a fluorescent reporter gene. Assumptions Specific to the ExperimentsThe previous experiment is used to find parameters regarding to our modelization. However, the experiments themselves must be described in the same way to involve those parameters in a consistent way, and actually, to be interpreted.
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