Team:Groningen

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@import url("http://igemgroningen.nerbonne.org/igem2008/igemsite.css");
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This is a template page. READ THESE INSTRUCTIONS.
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      <img src="https://static.igem.org/mediawiki/2008/c/cd/Groningen2008_Banner.jpg" id="banner"/>
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      <div id="menu">
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        <ul class="L1">
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          <li>
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            <a href="https://2008.igem.org/Team:Groningen">Home</a>
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          </li>
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          <li>
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            <a href="https://2008.igem.org/Team:Groningen/Introduction">Introduction</a>
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          </li>
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          <li class="sub">
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            <a href="https://2008.igem.org/Team:Groningen/design.html">Design</a>
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            <ul class="L2">
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              <li class="sub"><a href="https://2008.igem.org/Team:Groningen/design.html#interval_switch">Interval Switch</a></li>
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              <li class="sub"><a href="https://2008.igem.org/Team:Groningen/design.html#genetic_circuit">Genetic Circuit</a></li>
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              <li class="sub"><a href="https://2008.igem.org/Team:Groningen/design.html#physical_system">Physical System</a></li>
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            </ul>
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          <li class="sub">
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            <a href=""> Modeling </a>
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            <ul Class="L2">
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              <li class="sub"><a href="https://2008.igem.org/Team:Groningen/modeling_SingleCell.html"> Single-cell </a></li>
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              <li class="sub"><a href="https://2008.igem.org/Team:Groningen/modeling_Spatial.html"> Spatial model </a></li>
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<li class="sub"><a href="https://2008.igem.org/Team:Groningen/modeling_files.html">Model Files</a></li>
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            </ul>
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          <li class="sub">
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            <a href="https://2008.igem.org/Team:Groningen/results.html">Results</a>
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            <ul class="L2">
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              <li class="sub"><a href="https://2008.igem.org/Team:Groningen/results.html#results"> Results </a></li>
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              <li class="sub"><a href="https://2008.igem.org/Team:Groningen/results.html#conclusion"> Conclusion </a></li>
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<li class="sub"><a href="https://2008.igem.org/Team:Groningen/iGEM_Criteria">iGEM Criteria</a></li>
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            </ul>
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          </li>
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          <li class="sub">
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            <a href="">About...</a>
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            <ul class="L2">
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              <li class="sub"><a href="https://2008.igem.org/Team:Groningen/team.html"> The Team </a></li>
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              <li class="sub"><a href="https://2008.igem.org/Team:Groningen/rug.html"> Groningen University </a></li>
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              <li class="sub"><a href="https://2008.igem.org/Team:Groningen/references.html"> References </a></li>
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              <li class="sub"><a href="https://2008.igem.org/Team:Groningen/acknowledgements.html"> Acknowledgements </a></li>
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            </ul>
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          </li>
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        </ul>
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      </div>
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    </div>
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<div id="home" class="location">
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      <h1>Conway’s Game of Life in real life</h1>
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      <p>Conway’s Game of Life is a simple cellular automaton famous for generating complex ‘life-like’ patterns. The goal of this project is to explore the possibility of implementing cellular automata, particularly the Game of Life, as a regular spatial arrangement of bacteria. Communicating the number of neighbors is implemented using the well-known Homoserine Lactone (HSL) quorum sensing system. A novel component is the circuit implementing the automaton’s ruleset, to determine the state to switch to upon detecting ‘too few’, ‘enough’ or ‘too many’ neighbors. This ‘interval switch’ was designed and implemented by altering the binding site affinity of the signal molecule complexes to correspond to the levels of HSL coming from the neighbors. Finally, the ON state of the cells is indicated by GFP production and production of new HSL signals, and the OFF state by their absence. The system was implemented partially in vivo and we have developed in silico models.</p>
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<div id="instructions" style="text-align: center; font-weight: normal; font-size: small; color: #f6f6f6; padding: 5px;">
 
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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.
 
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You <strong>MUST</strong> have a team description page, a project abstract, a complete project description, and a lab notebook.  PLEASE keep all of your pages within your Team:Example namespace. 
 
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{|align="justify"
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|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
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|[[Image:Example_logo.png|200px|right|frame]]
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''Tell us more about your project.  Give us background.  Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
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|[[Image:Team.png|right|frame|Your team picture]]
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|align="center"|[[Team:Groningen | Team Example 2]]
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
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!align="center"|[[Team:Groningen|Home]]
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!align="center"|[[Team:Groningen/Team|The Team]]
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!align="center"|[[Team:Groningen/Project|The Project]]
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!align="center"|[[Team:Groningen/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:Groningen/Modeling|Modeling]]
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!align="center"|[[Team:Groningen/Notebook|Notebook]]
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(''Or you can choose different headings.  But you must have a team page, a project page, and a notebook page.'')
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Latest revision as of 03:56, 30 October 2008

Conway’s Game of Life in real life

Conway’s Game of Life is a simple cellular automaton famous for generating complex ‘life-like’ patterns. The goal of this project is to explore the possibility of implementing cellular automata, particularly the Game of Life, as a regular spatial arrangement of bacteria. Communicating the number of neighbors is implemented using the well-known Homoserine Lactone (HSL) quorum sensing system. A novel component is the circuit implementing the automaton’s ruleset, to determine the state to switch to upon detecting ‘too few’, ‘enough’ or ‘too many’ neighbors. This ‘interval switch’ was designed and implemented by altering the binding site affinity of the signal molecule complexes to correspond to the levels of HSL coming from the neighbors. Finally, the ON state of the cells is indicated by GFP production and production of new HSL signals, and the OFF state by their absence. The system was implemented partially in vivo and we have developed in silico models.

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