Team:Tsinghua/Project2

From 2008.igem.org

(Difference between revisions)
(PHA Project)
 
(7 intermediate revisions not shown)
Line 1: Line 1:
 +
<!--- The Mission, Experiments --->
 +
[[image:331.jpeg|center|]]
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="100%" align="center"
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="100%" align="center"
!align="center"|[[Team:Tsinghua|HOME]]
!align="center"|[[Team:Tsinghua|HOME]]
Line 17: Line 19:
&nbsp;&nbsp;&nbsp;[[WetLab Results]]
&nbsp;&nbsp;&nbsp;[[WetLab Results]]
 +
 +
&nbsp;&nbsp;&nbsp;[[PHA Project Modeling]]
&nbsp;&nbsp;&nbsp;[[Reference]]
&nbsp;&nbsp;&nbsp;[[Reference]]
-
== Reference ==
 
-
 
-
 
-
 
-
 
-
1
 
-
A sensitive, viable-colony staining method using Nile red
 
-
for direct screening of bacteria that accumulate polyhydroxyalkanoic
 
-
acids and other lipid storage compounds. Patricia Spiekermann · Bernd H. A. Rehm ·
 
-
Rainer Kalscheuer · Dirk Baumeister ·
 
-
Alexander Steinbüchel. Arch Microbiol (1999) 171:73–80
 
-
 
-
'''This literature explains how pBHR68 works and how PHA granules are detected.'''
 
-
 
-
2  Construction and Selection of  the  Novel  Recombinant Escherichia cob Strain  for Poly(Hydroxybutyrate)  Production.HUIMIN YU,JIN  YIN,HONGQI LI,SHENGLI  YANG,AND  ZHONGYAO. JOURNALOF BIOSCIENCEAND BIOENGWEERING
 
-
Vol. 89,  No. 4, 307-311.  2000
 
-
 
-
'''This literature indicates how PHA sysnthesis is realized in Escherichia coli.'''
 
-
 
-
3 Spontaneous liberation of intracellular polyhydroxybutyrate granules in Escherichia coli.Il Lae Jung, Ki Heon Phyo, Kug Chan Kim, Hyo Kook Park, In Gyu Kim.Research in Microbiology 156 (2005) 865–873
 
-
 
-
'''This literature introduced a method to release PHB granules from Escherichia coli.'''
 
-
 
-
4 Simultaneous Expression of Vitreoscilla Globin Gene and Lytic Genes of Phage lambda Novel RecombinantEscherichia Coli Used for Production of PHB.YU Huimin,SHI Yue,YIN Jin,and SHEN Zhongyao.ChineseJ.ofChem.Eug,(4)407一411(2001)
 
-
 
-
'''This literature introduced phage lambda lytic gene into Escherichia coli strains.'''
 
-
 
-
5 Autoregulator Protein PhaR for Biosynthesis of Polyhydroxybutyrate [P(3HB)] Possibly Has Two Separate Domains That Bind to the Target DNA and P(3HB): Functional Mapping of Amino Acid Residues Responsible for DNA Binding.Miwa Yamada,Koichi Yamashita,Akiko Wakuda,Kazuyoshi Ichimura,Akira Maehara,Michihisa Maeda,and Seiichi Taguchi.JOURNAL  OF  BACTERIOLOGY, Feb. 2007, p. 1118–1127
 
-
 
-
'''This literature illustrates how PhaR works in the PHB synthesis regulation.'''
 
-
 
-
6 Influence  of  homologous  phasins  (PhaP)  on PHA  accumulation  and  regulation  of  their expression  by  the  transcriptional  repressor PhaR  in  Ralstonia  eutropha  H16.Markus  Po¨tter,  Helena  Muller  and  Alexander  Steinbuchel.Microbiology  (2005),  151,  825–833.
 
-
'''This literature illustrates in the interaction of PhaP and PhaR.'''
 
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="100%" align="center"
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="100%" align="center"
Line 65: Line 36:
!align="center"|[[Team:Tsinghua/Doodle|Doodle Board]]
!align="center"|[[Team:Tsinghua/Doodle|Doodle Board]]
|}
|}
 +
<!--- The Mission, Experiments --->
 +
[[image:06.jpeg|center|]]

Latest revision as of 04:30, 30 October 2008

331.jpeg
HOME Team Project 1 Project 2 Parts Modelling Notebook Doodle Board

PHA Project

   Project Description

   Designed Charts

   Molecular Cloning

   WetLab Results

   PHA Project Modeling

   Reference


HOME Team Project 1 Project 2 Parts Modelling Notebook Doodle Board
06.jpeg