Team:HKUSTers/Initial Proposal/Randomizer

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The core circuit design

Two T7 promoters overlap each other, so that the first RNA polymerase binding event could be random. Coupling with downstream reporters, the random machine will give either 1 or 0 signal (presenting by GFP or RFP). To make the system robust, we include repressor on both left and right side. Repressor on each side will shun down the gene transcription on the other side. Therefore, once one side being turned on, it will shun down the other side.

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 ==The core circuit design==
-Two promoters locate adjacent to each other, so that the first RNA polymerase binding event could be random if two promoters have similar binding affinity. And this will generate establishing effect; one gene products will have higher copy number, which could effectively repress the neighbor promoter, and further induce its own expression, thus the optimal result is that either Blue or Red gene is dominated.
+Two T7 promoters overlap each other, so that the first RNA polymerase binding event could be random. Coupling with downstream reporters, the random machine will give either 1 or 0 signal (presenting by GFP or RFP). To make the system robust, we include repressor on both left and right side. Repressor on each side will shun down the gene transcription on the other side. Therefore, once one side being turned on, it will shun down the other side.
-[[Image:Promoter 1.png]]
-==Background Information==
--35 and -10 are important sites for polymerase binding, cI protein is generated by cI gene, which firstly binds to OR1, repress PR, and cooperatively bind to OR2 induce PRM thus its own production.
-[[Image:Promoter.png]]
-==