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User:University of Washington/25 June 2008
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== Yeast Shuttle Plasmid == | == Yeast Shuttle Plasmid == | ||
| + | - An agar plate containing yeast strain BY4741 (MATalpha, ΔBar1::Kan MX, his3Δ1; leu2Δ0; met15Δ0; ura3Δ0) was obtained. | ||
| - | - | + | - The yeast was incubated overnight at 30 degrees Celsius. |
| - | + | ||
| - | + | ||
| - | + | ||
== LuxR under AraC and TetR == | == LuxR under AraC and TetR == | ||
| - | -Obtained BBa_R0040 (TetR), BBa_R0080 (AraC) | + | - Obtained BBa_R0040 (TetR), BBa_R0080 (AraC) |
| - | -transformed and plated with Amp | + | - transformed and plated with Amp |
== LuxR under pLac == | == LuxR under pLac == | ||
| - | -Obtained parts R0010, I0462, and J04430. | + | - Obtained parts R0010, I0462, and J04430. |
| + | |||
| + | - R0010 and I0462 were transformed into MG strains, and plated on Amp. | ||
| - | - | + | - J04430 was transformed into DH strain, and plated on Amp. |
| - | |||
| - | [[Team:University_of_Washington/Notebook#Notebook]] | + | ---- |
| + | Back to [[Team:University_of_Washington/Notebook#Notebook]] | ||
Latest revision as of 00:25, 1 July 2008
Yeast Shuttle Plasmid
- An agar plate containing yeast strain BY4741 (MATalpha, ΔBar1::Kan MX, his3Δ1; leu2Δ0; met15Δ0; ura3Δ0) was obtained.
- The yeast was incubated overnight at 30 degrees Celsius.
LuxR under AraC and TetR
- Obtained BBa_R0040 (TetR), BBa_R0080 (AraC)
- transformed and plated with Amp
LuxR under pLac
- Obtained parts R0010, I0462, and J04430.
- R0010 and I0462 were transformed into MG strains, and plated on Amp.
- J04430 was transformed into DH strain, and plated on Amp.
Back to Team:University_of_Washington/Notebook#Notebook
