Team:Chiba/Calendar-Home/23 August 2008

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22 August 2008 <|> 24 August 2008

Meeting with UmeG

the ppt for our weekly meeting(japanese)

1. Team:Input
2. Team:Communication
3. team:Output

Laboratory work

Team:Input

no work

Team:Communication

(21/8)--->Mini prep

• [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2007)
• [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2006)
• [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2007)
• [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2006)
• [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2007)
• [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2006)

--->Digestion test

1. [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2007)
2. [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2006)
3. [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2007)
4. [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2006)
5. [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2007)
6. [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2006)
7. [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)

Sample No.	1- 6	7	1 - 7	1 - 7
DNA(μL)	3	1	3	3
EcoRI(μL)	-	-	0.1	0.1
PstI(μL)	-	-	0.1	0.1
Buffer EcoRI(μL)	-	-	0.9	0.8
BSA(μL)	-	-	1	1
dH2O(μL)	-	-	5	5
TOTAL(μL)	3	1	10	10

--->Gel Check

Sample No. 1~6 7 Sample DNA 3 1 Loading Dye(μL) 1 1 dH2O(μL) 2 4 TOTAL(μL) 6 6 From left; Sanple No. 1~7 [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2007) [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2006) [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2007) [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2006) [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2007) [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2006) [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)

Sample No. 8~21 Sample DNA(μL) 10 Loading Dye(μL) 2 TOTAL(μL) 12 From left; (Single Digestion) Sanple No. 8~14 [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2007) -> OK [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2006) -> OK [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2007) -> OK [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2006) -> OK [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2007) -> OK [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2006) -> OK [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007) -> OK (Double Digestion) Sample No. 15~21 [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2007) -> OK [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2006) -> OK [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2007) -> OK [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2006) -> OK [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2007) -> OK [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2006) -> OK [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007) -> OK?

Digestion test

[http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007:old-sample)

Sample No.	1	2	3
Sample DNA(μL)	3	3	3
EcoRI(μL)	-	0.1	0.1
SpeI(μL)	-	-	0.1
EcoRI Buffer(μL)	-	0.9	0.8
BSA(μL)	-	1	1
dH2O(μL)	-	5	5
TOTAL(μL)	3	10	10

--->Gel Check

Sample No. 1 2 3 Sample DNA 3 10 10 Loading Dye(μL) 1 2 2 dH2O(μL) 2 - - TOTAL(μL) 6 12 12 From left; [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007) -> OK [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)Single Digestion -> OK [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)Double Digestion -> Bad

Team:Output

no work

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