Team:Chiba/Parts/j

From 2008.igem.org

(Difference between revisions)

Latest revision as of 07:22, 14 December 2008

We submitted these parts

Autoinducer synthase

parts usages

[http://partsregistry.org/Part:BBa_K084007 BBa_K084007]([http://partsregistry.org/Part:BBa_J04500 plac+rbs]+[http://partsregistry.org/wiki/index.php?title=Part:BBa_C0178 LasI(no LVA)])

LasI (3OC12HSL synthesizing protein from Pseudomonas aeruginosa) expression induced by IPTG.

[http://partsregistry.org/Part:BBa_K084008 BBa_K084008]([http://partsregistry.org/Part:BBa_J04500 plac+rbs]+[http://partsregistry.org/Part:BBa_C0170 RhlI(no LVA)]) and [http://partsregistry.org/Part:BBa_K084009 BBa_K084009]([http://partsregistry.org/Part:BBa_R0010 plac]+[http://partsregistry.org/Part:BBa_I9026 rbs+RhlI])

RhlI (C4HSL,C6HSL synthesizing protein from Pseudomonas aeruginosa) expression induced by IPTG.

[http://partsregistry.org/Part:BBa_K084010 BBa_K084010]([http://partsregistry.org/Part:BBa_R0010 plac]+[http://partsregistry.org/Part:BBa_I9030 rbs+CinI])

CinI (3-hydroxy-C14HSL synthesizing protein from Rhizobium leguminosarum) expression induced by IPTG.

[http://partsregistry.org/Part:BBa_K084012 BBa_K084012]([http://partsregistry.org/Part:BBa_J04500 plac+rbs]+[http://partsregistry.org/wiki/index.php?title=Part:BBa_C0161 LuxI(no LVA)]) and [http://partsregistry.org/Part:BBa_K084014 BBa_K084014]([http://partsregistry.org/Part:BBa_R0010 plac]+[http://partsregistry.org/wiki/index.php?title=Part:BBa_C0261 rbs+LuxI])

Lux I (3OC6HSL synthesizing protein from Vibrio Fischeri) expression induced by IPTG.

Transcriptional Regulator

[http://partsregistry.org/Part:BBa_K084004 BBa_K084004] ([http://partsregistry.org/Part:BBa_R0040 BBa_R0040]+[http://partsregistry.org/Part:BBa_S03158 BBa_S03158])

Transcriptional regulator RhlR (BBa_C0071) generator regulated by tet promoter

[http://partsregistry.org/Part:BBa_K084005 BBa_K084005] ([http://partsregistry.org/Part:BBa_R0040 BBa_R0040]+[http://partsregistry.org/Part:BBa_S03156 BBa_S03156])

Transcriptional regulator LasR (BBa_C0079) generator regulated by tet promoter

[http://partsregistry.org/Part:BBa_K084006 BBa_K084006]

([http://partsregistry.org/Part:BBa_R0040 BBa_R0040]+[http://partsregistry.org/Part:BBa_S03160 BBa_S03160])

Transcriptional regulator CinR (BBa_C0077) generator regulated by tet promoter

Reporters

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084003 BBa_K084003]

3OC6HSL -> Venus YFP Venus YFP Producer Controlled by 3OC6HSL Receiver

@@ Line 1: / Line 1: @@
->[[Team:Chiba/j| ホーム]]
+<html><link rel="stylesheet" href="https://2008.igem.org/wiki/index.php?title=User:Maiko/chiba.css&action=raw&ctype=text/css" type="text/css" /></html>
+[[Image:Chiba-U.gif|center]]
-We hope to submit these parts:
+{| style="color:white;" cellpadding="3" cellspacing="3" border="0" width="100%" align="center" class="menu" |
+!align="center"|[[Team:Chiba/j|ホーム]]
+!align="center"|[[Team:Chiba/Team/j|メンバー紹介]]
+!align="center"|[[Team:Chiba/Project/j|プロジェクト紹介]]
+!align="center"|[[Team:Chiba/Parts/j|作成したDNAパーツ]]
+!align="center"|[[Team:Chiba/Internal|ノート]]
+|}
+__NOTOC__
+==We submitted these parts==
 ===Autoinducer synthase===
-*BBa_K084007 [http://partsregistry.org/Part:BBa_K084007]<Br>lacI promotor regulate lasI gene for an autoinducer synthetase for PAI from Pseudomonas aeruginosa(no LVA).
+==== parts usages ====
-*BBa_K084008 [http://partsregistry.org/Part:BBa_K084008]<Br>lacI promotor regulate rhlI gene for an autoinducer synthetase for N-butyryl-HSL (BHL) and HHL(no LVA).
+*[http://partsregistry.org/Part:BBa_K084007 BBa_K084007]([http://partsregistry.org/Part:BBa_J04500 plac+rbs]+[http://partsregistry.org/wiki/index.php?title=Part:BBa_C0178 LasI(no LVA)])
-*BBa_K084009 [http://partsregistry.org/Part:BBa_K084009]<Br>lacI promotor regulate rhlI gene with LVA.
+[[Image:Tet-lasi chiba.gif]]
-*BBa_K084010 [http://partsregistry.org/Part:BBa_K084010]<Br>lacI promotor regulate cinI gene with LVA that is for an autoinducer synthetase which utilizes SAM to make the quorum sensing molecule 3OH,C14:1-HSL.
-*BBa_K084012 [http://partsregistry.org/Part:BBa_K084012]<Br>Generates an untagged LuxI enzyme which produces an AHL(3OC6HSL) under a lac-repressible promoter.
-*BBa_K084014 [http://partsregistry.org/Part:BBa_K084014]<Br>Generates 3OC6-HSL under a lac-repressible promoter
-===Receiver protein===
+LasI (3OC12HSL synthesizing protein from ''Pseudomonas aeruginosa'') expression induced by IPTG.
-*BBa_K084004 [http://partsregistry.org/Part:BBa_K084004]
-*BBa_K084005 [http://partsregistry.org/Part:BBa_K084005]
-*BBa_K084006 [http://partsregistry.org/Part:BBa_K084006]
-===Reporter===
+*[http://partsregistry.org/Part:BBa_K084008 BBa_K084008]([http://partsregistry.org/Part:BBa_J04500 plac+rbs]+[http://partsregistry.org/Part:BBa_C0170 RhlI(no LVA)]) and [http://partsregistry.org/Part:BBa_K084009 BBa_K084009]([http://partsregistry.org/Part:BBa_R0010 plac]+[http://partsregistry.org/Part:BBa_I9026 rbs+RhlI])
-*BBa_K084003 [http://partsregistry.org/Part:BBa_K084003]
+[[Image:Tet-rhli chiba.gif]]
+RhlI (C4HSL,C6HSL synthesizing protein from ''Pseudomonas aeruginosa'') expression induced by IPTG.
+*[http://partsregistry.org/Part:BBa_K084010 BBa_K084010]([http://partsregistry.org/Part:BBa_R0010 plac]+[http://partsregistry.org/Part:BBa_I9030 rbs+CinI])
-{| style="color:white;background-color:Maroon" cellpadding="3" cellspacing="3" border="1" bordercolor="white" width="100%" align="center"
+CinI (3-hydroxy-C14HSL synthesizing protein from ''Rhizobium leguminosarum'') expression induced by IPTG.
+*[http://partsregistry.org/Part:BBa_K084012 BBa_K084012]([http://partsregistry.org/Part:BBa_J04500 plac+rbs]+[http://partsregistry.org/wiki/index.php?title=Part:BBa_C0161 LuxI(no LVA)]) and [http://partsregistry.org/Part:BBa_K084014 BBa_K084014]([http://partsregistry.org/Part:BBa_R0010 plac]+[http://partsregistry.org/wiki/index.php?title=Part:BBa_C0261 rbs+LuxI])
+[[Image:Tet-luxi chiba.gif]]
+Lux I (3OC6HSL synthesizing protein from ''Vibrio Fischeri'') expression induced by IPTG.
+===Transcriptional Regulator===
+*[http://partsregistry.org/Part:BBa_K084004 BBa_K084004]  ([http://partsregistry.org/Part:BBa_R0040  BBa_R0040]+[http://partsregistry.org/Part:BBa_S03158 BBa_S03158])
+[[Image:RhlR-p15A Chiba.gif]]
+Transcriptional regulator RhlR (BBa_C0071) generator regulated by tet promoter
+*[http://partsregistry.org/Part:BBa_K084005 BBa_K084005]  ([http://partsregistry.org/Part:BBa_R0040  BBa_R0040]+[http://partsregistry.org/Part:BBa_S03156 BBa_S03156])
+[[Image:LasR-p15A Chiba.gif]]
+Transcriptional regulator LasR (BBa_C0079) generator regulated by tet promoter
+*[http://partsregistry.org/Part:BBa_K084006 BBa_K084006]
+([http://partsregistry.org/Part:BBa_R0040  BBa_R0040]+[http://partsregistry.org/Part:BBa_S03160 BBa_S03160])
+[[Image:CinR-p15A Chiba.gif]]
+Transcriptional regulator CinR (BBa_C0077) generator regulated by tet promoter
+===Reporters===
+*[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084003 BBa_K084003]
+[[Image:K084003 chiba.gif]]
+OC6HSL -> Venus YFP   Venus YFP Producer Controlled by 3OC6HSL Receiver
+{| style="color:white;" cellpadding="3" cellspacing="3" border="0" width="100%" align="center" class="menu" |
 !align="center"|[[Team:Chiba/j|ホーム]]
 !align="center"|[[Team:Chiba/Team/j|メンバー紹介]]
 !align="center"|[[Team:Chiba/Project/j|プロジェクト紹介]]
-!align="center"|[[Team:Chiba/Parts/j|Parts Submitted to the Registry]]
+!align="center"|[[Team:Chiba/Parts/j|作成したDNAパーツ]]
-!align="center"|[[Team:Chiba/Modeling/j|モデリング]]
 !align="center"|[[Team:Chiba/Internal|ノート]]
 |}