Team:Chiba/jk/α

From 2008.igem.org

(Difference between revisions)
 
(45 intermediate revisions not shown)
Line 1: Line 1:
-
'''入力班のぺーじ。'''
+
<html><link rel="stylesheet" href="https://2008.igem.org/wiki/index.php?title=User:Maiko/chiba.css&action=raw&ctype=text/css" type="text/css" /></html>
 +
>[[Team:Chiba/Internal|ノートに戻る]]
 +
'''入力班のページ'''
-
== 2008.8.20==
+
[[Team:Chiba/notebook/input/august|8月の実験ログ]]
-
PCR:'''[[Team:Chiba/protocol/PCR|PCR]]'''
+
/[[Team:Chiba/notebook/input/september|9月の実験ログ]]
-
<BR>・BBa_J22136(2007)①
+
-
<BR>・BBa_J22136(2008)②
+
-
<BR>・BBa_J22141(2007)③
+
-
<BR>・BBa_J22141(2008)④
+
-
<BR>・BBa_E0612(2008)⑤
+
-
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
+
-
<tr>
+
-
<td width="257">Sample No</td>
+
-
<td>①</td><td>②</td><td>③</td><td>④</td><td>⑤</td>
+
-
</tr>
+
-
<tr>
+
-
<td>DNA tamplate</td>
+
-
<td>1</td><td>1</td><td>1</td><td>1</td>><td>1</td>
+
-
</tr>
+
-
<tr>
+
-
<td>FW primer</td>
+
-
<td>5</td><td>5</td><td>5</td><td>5</td><td>5</td>
+
-
</tr>
+
-
<tr>
+
-
<td>VR primer</td>
+
-
<td>5</td><td>5</td><td>5</td><td>5</td><td>5</td>
+
-
</tr>
+
-
<tr>
+
-
<td>dNTPmix</td>
+
-
<td>10</td><td>10</td><td>10</td><td>10</td><td>10</td>
+
-
</tr>
+
-
<tr>
+
-
<td>thermo pol buffer</td>
+
-
<td>10</td><td>10</td><td>10</td><td>10</td><td>10</td>
+
-
</tr>
+
-
<tr>
+
-
<td>DNA pol(VENT)</td>
+
-
<td>1</td><td>1</td><td>1</td><td>1</td><td>1</td>
+
-
</tr>
+
-
<tr>
+
-
<td>dH2O</td>
+
-
<td>68</td><td>68</td><td>68</td><td>68</td><td>68</td>
+
-
</tr>
+
-
<tr>
+
-
<td>TOTAL</td>
+
-
<td>100</td><td>100</td><td>100</td><td>100</td><td>100</td>
+
-
</tr>
+
-
<tr>
+
-
<td>TOTAL</td>
+
-
<td>6</td><td>6</td><td>6</td><td>6</td><td>6</td>
+
-
</tr>
+
-
</table>
+
-
<BR>→gel check:'''[[Team:Chiba/protocol/gel check|Gel Check]]'''
+
-
<table width="200" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
+
-
<tr>
+
-
<td width="257">Sample No</td>
+
-
<td>①</td><td>②</td><td>③</td><td>④</td><td>⑤</td>
+
-
</tr>
+
-
<tr>
+
-
<td>sample DNA</td>
+
-
<td>1</td><td>1</td><td>1</td><td>1</td>><td>1</td>
+
-
</tr>
+
-
<tr>
+
-
<td>loading dye</td>
+
-
<td>1</td><td>1</td><td>1</td><td>1</td><td>1</td>
+
-
</tr>
+
-
<tr>
+
-
<td>dH<sub>2</sub>O</td>
+
-
<td>4</td><td>4</td><td>4</td><td>4</td><td>4</td>
+
-
</tr>
+
-
<tr>
+
-
<td>total</td>
+
-
<td>6</td><td>6</td><td>6</td><td>6</td><td>6</td>
+
-
</tr>
+
-
</table>
 
-
→どれもバンド出ず。
 
-
 
+
{| style="color:white;" cellpadding="3" cellspacing="3" border="0" width="100%" align="center" class="menu" |
-
<BR>trnsformation:'''[[Team:Chiba/protocol/transformation|Transformation]]'''
+
-
 
+
-
<BR>・BBa_R0051(2007)
+
-
<BR>・BBa_R0051(2006)
+
-
<BR>・BBa_J06650(2007)
+
-
<BR>・BBa_J06650(2006)
+
-
<BR>・BBa_J22136(2007)
+
-
<BR>・BBa_J22141(2007)
+
-
 
+
-
→BBa_J22141のみコロニーがほとんど生えていなかった。(1個のみ)
+
-
 
+
-
== 2008.8.21 ==
+
-
8.20にtransformationしたもの
+
-
<BR>・BBa_R0051(2007)
+
-
<BR>・BBa_R0051(2006)
+
-
<BR>・BBa_J06650(2007)
+
-
<BR>・BBa_J06650(2006)
+
-
<BR>・BBa_J22136(2007)
+
-
<BR>・BBa_J22141(2007)
+
-
を2ml培養、12h。ただしコロニーが1個だけだったBBa_J22141は2ml培養の11h後にIPTGを20μl加え、
+
-
さらに1h培養した。
+
-
== 2008.8.22 ==
+
-
mini prep:'''[[Team:Chiba/protocol/mini prep|Mini Prep]]'''
+
-
2ml培養した
+
-
<BR>・BBa_R0051(2007)
+
-
<BR>・BBa_R0051(2006)
+
-
<BR>・BBa_J06650(2007)
+
-
<BR>・BBa_J06650(2006)
+
-
<BR>・BBa_J22136(2007)
+
-
<BR>・BBa_J22141(2007)
+
-
をミニプレした。
+
-
 
+
-
 
+
-
 
+
-
{| style="color:green;background-color:Maroon" cellpadding="3" cellspacing="3" border="1" bordercolor="white" width="100%" align="center"
+
!align="center"|[[Team:Chiba/j|ホーム]]
!align="center"|[[Team:Chiba/j|ホーム]]
!align="center"|[[Team:Chiba/Team/j|メンバー紹介]]
!align="center"|[[Team:Chiba/Team/j|メンバー紹介]]
!align="center"|[[Team:Chiba/Project/j|プロジェクト紹介]]
!align="center"|[[Team:Chiba/Project/j|プロジェクト紹介]]
-
!align="center"|[[Team:Chiba/Parts/j|Parts Submitted to the Registry]]
+
!align="center"|[[Team:Chiba/Parts/j|作成したDNAパーツ]]
-
!align="center"|[[Team:Chiba/Modeling/j|モデリング]]
+
!align="center"|[[Team:Chiba/Internal|ノート]]
!align="center"|[[Team:Chiba/Internal|ノート]]
 +
|}

Latest revision as of 11:26, 14 December 2008

>ノートに戻る

入力班のページ

8月の実験ログ /9月の実験ログ