Team:Chiba/jk/α

From 2008.igem.org

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'''入力班のぺーじ。'''
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<html><link rel="stylesheet" href="https://2008.igem.org/wiki/index.php?title=User:Maiko/chiba.css&action=raw&ctype=text/css" type="text/css" /></html>
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>[[Team:Chiba/Internal|ノートに戻る]]
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'''入力班のページ'''
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== 2008.8.20==
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[[Team:Chiba/notebook/input/august|８月の実験ログ]]
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PCR:'''[[Team:Chiba/protocol/PCR|PCR]]'''
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/[[Team:Chiba/notebook/input/september|９月の実験ログ]]
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<BR>・BBa_J22136(2007)①
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<BR>・BBa_J22136(2008)②
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<BR>・BBa_J22141(2007)③
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<BR>・BBa_J22141(2008)④
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<BR>・BBa_E0612(2008)⑤
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<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
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<tr>
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<td width="257">Sample No</td>
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<td>①</td><td>②</td><td>③</td><td>④</td><td>⑤</td>
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</tr>
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<tr>
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<td>DNA tamplate</td>
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<td>1</td><td>1</td><td>1</td><td>1</td>><td>1</td>
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</tr>
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<tr>
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<td>FW primer</td>
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<td>5</td><td>5</td><td>5</td><td>5</td><td>5</td>
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</tr>
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<tr>
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<td>VR primer</td>
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<td>5</td><td>5</td><td>5</td><td>5</td><td>5</td>
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</tr>
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<tr>
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<td>dNTPmix</td>
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<td>10</td><td>10</td><td>10</td><td>10</td><td>10</td>
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</tr>
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<tr>
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<td>thermo pol buffer</td>
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<td>10</td><td>10</td><td>10</td><td>10</td><td>10</td>
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</tr>
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<tr>
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<td>DNA pol(VENT)</td>
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<td>1</td><td>1</td><td>1</td><td>1</td><td>1</td>
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</tr>
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<tr>
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<td>dH2O</td>
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<td>68</td><td>68</td><td>68</td><td>68</td><td>68</td>
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</tr>
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<tr>
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<td>TOTAL</td>
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<td>100</td><td>100</td><td>100</td><td>100</td><td>100</td>
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</tr>
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<tr>
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<td>TOTAL</td>
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<td>6</td><td>6</td><td>6</td><td>6</td><td>6</td>
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</tr>
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</table>
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<BR>→gel check:'''[[Team:Chiba/protocol/gel check|Gel Check]]'''
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<table width="200" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
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<tr>
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<td width="257">Sample No</td>
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<td>①</td><td>②</td><td>③</td><td>④</td><td>⑤</td>
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</tr>
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<tr>
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<td>sample DNA</td>
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<td>1</td><td>1</td><td>1</td><td>1</td>><td>1</td>
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</tr>
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<tr>
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<td>loading dye</td>
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<td>1</td><td>1</td><td>1</td><td>1</td><td>1</td>
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</tr>
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<tr>
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<td>dH<sub>2</sub>O</td>
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<td>4</td><td>4</td><td>4</td><td>4</td><td>4</td>
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</tr>
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<tr>
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<td>total</td>
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<td>6</td><td>6</td><td>6</td><td>6</td><td>6</td>
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</tr>
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</table>
 
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→どれもバンド出ず。
 
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{| style="color:white;" cellpadding="3" cellspacing="3" border="0" width="100%" align="center" class="menu" |
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<BR>trnsformation:'''[[Team:Chiba/protocol/transformation|Transformation]]'''
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<BR>・BBa_R0051(2007)
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<BR>・BBa_R0051(2006)
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<BR>・BBa_J06650(2007)
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<BR>・BBa_J06650(2006)
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<BR>・BBa_J22136(2007)
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<BR>・BBa_J22141(2007)
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→BBa_J22141のみコロニーがほとんど生えていなかった。(１個のみ)
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== 2008.8.21 ==
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8.20にtransformationしたもの
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<BR>・BBa_R0051(2007)
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<BR>・BBa_R0051(2006)
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<BR>・BBa_J06650(2007)
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<BR>・BBa_J06650(2006)
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<BR>・BBa_J22136(2007)
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<BR>・BBa_J22141(2007)
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を２ml培養、12h。ただしコロニーが１個だけだったBBa_J22141は2ml培養の11h後にIPTGを20μl加え、
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さらに1h培養した。
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== 2008.8.22 ==
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mini prep:'''[[Team:Chiba/protocol/mini prep|Mini Prep]]'''
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<BR>2ml培養した
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<BR>・BBa_R0051(2007)
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<BR>・BBa_R0051(2006)
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<BR>・BBa_J06650(2007)
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<BR>・BBa_J06650(2006)
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<BR>・BBa_J22136(2007)
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<BR>・BBa_J22141(2007)
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<BR>をミニプレした。
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{| style="color:green;background-color:Maroon" cellpadding="3" cellspacing="3" border="1" bordercolor="white" width="100%" align="center"
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!align="center"|[[Team:Chiba/j|ホーム]]
!align="center"|[[Team:Chiba/j|ホーム]]
!align="center"|[[Team:Chiba/Team/j|メンバー紹介]]
!align="center"|[[Team:Chiba/Team/j|メンバー紹介]]
!align="center"|[[Team:Chiba/Project/j|プロジェクト紹介]]
!align="center"|[[Team:Chiba/Project/j|プロジェクト紹介]]
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!align="center"|[[Team:Chiba/Parts/j|Parts Submitted to the Registry]]
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!align="center"|[[Team:Chiba/Parts/j|作成したDNAパーツ]]
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!align="center"|[[Team:Chiba/Modeling/j|モデリング]]
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!align="center"|[[Team:Chiba/Internal|ノート]]
!align="center"|[[Team:Chiba/Internal|ノート]]
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|}

Latest revision as of 11:26, 14 December 2008

>ノートに戻る

入力班のページ

８月の実験ログ /９月の実験ログ



ホーム メンバー紹介 プロジェクト紹介 作成したDNAパーツ ノート
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