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Revision as of 01:29, 8 October 2008 (view source) Su4 (Talk | contribs) ← Older edit		Latest revision as of 06:22, 29 March 2016 (view source) 185.106.92.123 (Talk) (MfEQCIlenroHQYZG)
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-	{{bottom_template}}	+	mZgAkl  <a href="http://fbdnisdgysij.com/">fbdnisdgysij</a>, [url=http://jzowycxvfekb.com/]jzowycxvfekb[/url], [link=http://lkctxlgmquum.com/]lkctxlgmquum[/link], http://obyjjyzxsbxr.com/
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-	<!--PLACE TEXT BELOW HERE -->	+
-	== July 1, 2008 ==	+
-	Made Yeast Media	+
-		+
-	YPD Medium, per liter:	+
-		+
-	10g yeast extract	+
-		+
-	20g peptone	+
-		+
-	20g dextrose	+
-		+
-	20g agar(only for plates)	+
-		+
-	1. Dextrose filter sterilized, the rest autoclaved	+
-		+
-	2. Weigh nutrients into flask double the volume you want to make, and stir to dissolve	+
-		+
-	3. Dextrose added to autoclaved media to equivalent of 20 g/L	+
-		+
-	4. Liquid media placed on bench, plate media placed in 65 degree water bath approximately 5 minutes	+
-		+
-	5. Poured into plates and allowed to solidify	+
-		+
-		+
-	== July 17, 2008 ==	+
-	E.Coli Media	+
-		+
-	LB medium, per liter	+
-		+
-	10g tryptone	+
-		+
-	5g yeast extract	+
-		+
-	5g NaCl	+
-		+
-	1 mL 1N NaOH	+
-		+
-	15g (agar for plates)	+
-		+
-	1. Antibody DNA resuspended in TE buffer, 0.1 μg/μL	+
-		+
-	2. 5mL LB inoculated with single colony DH5a pro	+
-		+
-	3. Incubated at 37 degrees overnight	+
-		+
-		+
-	== July 18, 2008 ==	+
-	Competent cells:	+
-		+
-	1. 3mL overnight culture of DH5a pro	+
-		+
-	2. Inoculated into 35mL of LB	+
-		+
-	3. OD600 checked, want 0.2-0.3	+
-		+
-	4. Place culture on ice for 3 minutes	+
-		+
-	5. Spin at 10,000 rpm for 7 minutes, discard supernatant	+
-		+
-	6. Resuspend in 10 mL cold 30 mM CaCl2, incubated on ice overnight	+
-	<!-- == Insert Date Here ==	+
-	* lab procedure	+
-	** more lab procedure	+
-	* second lab procedure	+
-	** more about second lab procedure	+
-	COPY/PASTE THEN REMOVE THIS AND BRACKETS-->	+
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-	{{bottom_template}}	+

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Latest revision as of 06:22, 29 March 2016

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