Team:Illinois/Antibody Receptor Tyrosine Kinase Fusion Notebook

From 2008.igem.org

(Difference between revisions)
(MfEQCIlenroHQYZG)
 
(73 intermediate revisions not shown)
Line 1: Line 1:
-
{{bottom_template}}
+
mZgAkl  <a href="http://fbdnisdgysij.com/">fbdnisdgysij</a>, [url=http://jzowycxvfekb.com/]jzowycxvfekb[/url], [link=http://lkctxlgmquum.com/]lkctxlgmquum[/link], http://obyjjyzxsbxr.com/
-
 
+
-
 
+
-
<!--PLACE TEXT BELOW HERE -->
+
-
== July 1, 2008 ==
+
-
Made Yeast Media
+
-
 
+
-
YPD Medium, per liter:
+
-
 
+
-
10g yeast extract
+
-
 
+
-
20g peptone
+
-
 
+
-
20g dextrose
+
-
 
+
-
20g agar(only for plates)
+
-
 
+
-
1. Dextrose filter sterilized, the rest autoclaved
+
-
 
+
-
2. Weigh nutrients into flask double the volume you want to make, and stir to dissolve
+
-
 
+
-
3. Dextrose added to autoclaved media to equivalent of 20 g/L
+
-
 
+
-
4. Liquid media placed on bench, plate media placed in 65 degree water bath approximately 5 minutes
+
-
 
+
-
5. Poured into plates and allowed to solidify
+
-
 
+
-
 
+
-
== July 17, 2008 ==
+
-
E.Coli Media
+
-
 
+
-
LB medium, per liter
+
-
 
+
-
10g tryptone
+
-
 
+
-
5g yeast extract
+
-
 
+
-
5g NaCl
+
-
 
+
-
1 mL 1N NaOH
+
-
 
+
-
15g (agar for plates)
+
-
 
+
-
1. Antibody DNA resuspended in TE buffer, 0.1 μg/μL
+
-
 
+
-
2. 5mL LB inoculated with single colony DH5a pro
+
-
 
+
-
3. Incubated at 37 degrees overnight
+
-
 
+
-
 
+
-
== July 18, 2008 ==
+
-
Competent cells:
+
-
 
+
-
1. 3mL overnight culture of DH5a pro
+
-
 
+
-
2. Inoculated into 35mL of LB
+
-
 
+
-
3. OD600 checked, want 0.2-0.3
+
-
 
+
-
4. Place culture on ice for 3 minutes
+
-
 
+
-
5. Spin at 10,000 rpm for 7 minutes, discard supernatant
+
-
 
+
-
6. Resuspend in 10 mL cold 30 mM CaCl2, incubated on ice overnight
+
-
<!-- == Insert Date Here ==
+
-
* lab procedure
+
-
** more lab procedure
+
-
* second lab procedure
+
-
** more about second lab procedure
+
-
COPY/PASTE THEN REMOVE THIS AND BRACKETS-->
+
-
 
+
-
{{bottom_template}}
+

Latest revision as of 06:22, 29 March 2016

mZgAkl <a href="http://fbdnisdgysij.com/">fbdnisdgysij</a>, [url=http://jzowycxvfekb.com/]jzowycxvfekb[/url], [link=http://lkctxlgmquum.com/]lkctxlgmquum[/link], http://obyjjyzxsbxr.com/