Team:University of Alberta

From 2008.igem.org

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<P ALIGN=CENTER STYLE="background: #000000"><FONT COLOR="#00ff00"><FONT FACE="Estrangelo Nisibin Outline"><FONT SIZE=7 STYLE="font-size: 54pt"><B>The
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Chimeras</B></FONT></FONT></FONT></P>
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<P ALIGN=CENTER STYLE="background: #000000"><FONT COLOR="#00ff00"><FONT FACE="Estrangelo Nisibin Outline"><FONT SIZE=7 STYLE="font-size: 54pt"><B><FONT SIZE=5 STYLE="font-size: 20pt">(Blurring
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This is a template page. READ THESE INSTRUCTIONS.
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the line between plant and bacteria)</FONT> </B></FONT></FONT></FONT>
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<P ALIGN=CENTER STYLE="background: #000000"><BR><BR>
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You are provided with this team page template with which to start the iGEM season. You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.
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<P ALIGN=LEFT STYLE="background: #000000"><FONT COLOR="#00ff00"><FONT FACE="Estrangelo Nisibin Outline"><FONT SIZE=4 STYLE="font-size: 15pt"><B>A
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Brief Introduction</B></FONT></FONT></FONT></P>
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You <strong>MUST</strong> have a team description page, a project abstract, a complete project description, and a lab notebook.  PLEASE keep all of your pages within your Team:Example namespace. 
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<P ALIGN=LEFT STYLE="background: #000000"><FONT COLOR="#00ff00"><FONT FACE="Estrangelo Nisibin Outline"><FONT SIZE=4 STYLE="font-size: 15pt"><B>Our
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project focuses on creating the very first synthetic biology plant
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chassis ever presented as a IGEM project. We plan on doing this by
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taking advantage of already existing photosensors and temperature
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switches existing in plants. Our end result will be photo and
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temperature sensitive grass. The idea would be to introduce two
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different lux operons encoding for two different colors and have the
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expression of them controlled by light and temperature. During the
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day both lux pathways would be turned off by the photosensor switch,
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{|align="justify"
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at night the one of the lux operons would be turned on decided by the
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|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
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temperature switch.</B></FONT></FONT></FONT></P>
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</P>
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<P ALIGN=LEFT STYLE="background: #000000"><FONT COLOR="#00ff00"><FONT FACE="Estrangelo Nisibin Outline"><FONT SIZE=4 STYLE="font-size: 15pt"><B>The
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''Tell us more about your project.  Give us background.  Use this is the abstract of your project. Be descriptive but concise (1-2 paragraphs)''
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Photo Sensor</B></FONT></FONT></FONT></P>
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|[[Image:Team.png|right|frame|Your team picture]]
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<P ALIGN=LEFT STYLE="background: #000000"><FONT COLOR="#00ff00"><FONT FACE="Estrangelo Nisibin Outline"><FONT SIZE=4 STYLE="font-size: 15pt"><B>Our
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photo sensor would take advantage of an already existing red/far red
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pathway existing in plants. Pathway is normally used to detect
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|align="center"|[[Team:University_of_Alberta | Team Example 2]]
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shading by other plants as well as the end of the day. When red light
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is present the PR protein turns into PFR which activates another
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protein which then binds to LRE (Light Responsive Element) section of
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<!--- The Mission, Experiments --->
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the DNA this then activates the production of rubisco. When the plant
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is exposed to far red light (such as would occur at the end of the
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day) the PFR protien converts back to PR and the pathway shuts off.
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!align="center"|[[Team:University_of_Alberta|Home]]
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Our project will take advantage of this pathway and create an LRE
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!align="center"|[[Team:University_of_Alberta/Team|The Team]]
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biobrick which can then be used as a photosensor. In our project LRE
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!align="center"|[[Team:University_of_Alberta/Project|The Project]]
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will be attached to a luxR to allow for the lux pathway to be shut
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!align="center"|[[Team:University_of_Alberta/Parts|Parts Submitted to the Registry]]
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off during the day and turn on during the night. </B></FONT></FONT></FONT>
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!align="center"|[[Team:University_of_Alberta/Modeling|Modeling]]
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</P>
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!align="center"|[[Team:University_of_Alberta/Notebook|Notebook]]
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(''Or you can choose different headings.  But you must have a team page, a project page, and a notebook page.'')
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<P ALIGN=LEFT STYLE="background: #000000"><FONT COLOR="#00ff00"><FONT FACE="Estrangelo Nisibin Outline"><FONT SIZE=4 STYLE="font-size: 15pt"><B>The
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Temperature Sensor</B></FONT></FONT></FONT></P>
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<P ALIGN=LEFT STYLE="background: #000000"><FONT COLOR="#00ff00"><FONT FACE="Estrangelo Nisibin Outline"><FONT SIZE=4 STYLE="font-size: 15pt"><B>The
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temperature sensor will be cold sensitive that will cause the
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production of a repressor which will be used to repress one of the
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pathways (lux2). The repressed lux2 pathway carries another repressor
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for the lux1 pathway that is transcribed only when the temperature
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senor is turned off. This will allow for the change of color due to
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temperature. </B></FONT></FONT></FONT>
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</P>
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<P ALIGN=LEFT STYLE="background: #000000"><IMG SRC="/home/botany_rulz/Documents/html_html_m15df74ee.gif" NAME="Object1" ALIGN=LEFT WIDTH=1191 HEIGHT=378 BORDER=0><BR><BR>
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</P>
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</P>
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</P>
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<P ALIGN=LEFT STYLE="background: #000000"><FONT COLOR="#00ff00"><FONT FACE="Estrangelo Nisibin Outline"><FONT SIZE=4 STYLE="font-size: 15pt"><B>Our
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Team </B></FONT></FONT></FONT>
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</P>
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<P ALIGN=LEFT STYLE="background: #000000"><FONT COLOR="#00ff00"><FONT FACE="Estrangelo Nisibin Outline"><FONT SIZE=4 STYLE="font-size: 15pt"><B>Full
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Time</B></FONT></FONT></FONT></P>
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<P ALIGN=LEFT STYLE="background: #000000"><FONT COLOR="#00ff00"><FONT FACE="Estrangelo Nisibin Outline"><FONT SIZE=4 STYLE="font-size: 15pt"><B>Part
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Time </B></FONT></FONT></FONT>
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</P>
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</P>
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<P ALIGN=LEFT STYLE="background: #000000"><FONT COLOR="#00ff00"><FONT FACE="Estrangelo Nisibin Outline"><FONT SIZE=4 STYLE="font-size: 15pt"><B>The
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Lab Book</B></FONT></FONT></FONT></P>
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<P ALIGN=LEFT STYLE="background: #000000"><BR><BR>
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</P>
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<P ALIGN=LEFT STYLE="background: #000000"><FONT COLOR="#00ff00"><FONT FACE="Estrangelo Nisibin Outline"><FONT SIZE=4 STYLE="font-size: 15pt"><B>Our
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Sponsors</B></FONT></FONT></FONT></P>
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<P ALIGN=LEFT STYLE="background: #000000"><BR><BR>
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</P>
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<P ALIGN=LEFT STYLE="background: #000000"><FONT COLOR="#00ff00"><FONT FACE="Estrangelo Nisibin Outline"><FONT SIZE=4 STYLE="font-size: 15pt"><B>Special
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Thanks </B></FONT></FONT></FONT>
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Revision as of 16:19, 15 May 2008

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The Chimeras

(Blurring the line between plant and bacteria)



A Brief Introduction

Our project focuses on creating the very first synthetic biology plant chassis ever presented as a IGEM project. We plan on doing this by taking advantage of already existing photosensors and temperature switches existing in plants. Our end result will be photo and temperature sensitive grass. The idea would be to introduce two different lux operons encoding for two different colors and have the expression of them controlled by light and temperature. During the day both lux pathways would be turned off by the photosensor switch, at night the one of the lux operons would be turned on decided by the temperature switch.



The Photo Sensor

Our photo sensor would take advantage of an already existing red/far red pathway existing in plants. Pathway is normally used to detect shading by other plants as well as the end of the day. When red light is present the PR protein turns into PFR which activates another protein which then binds to LRE (Light Responsive Element) section of the DNA this then activates the production of rubisco. When the plant is exposed to far red light (such as would occur at the end of the day) the PFR protien converts back to PR and the pathway shuts off. Our project will take advantage of this pathway and create an LRE biobrick which can then be used as a photosensor. In our project LRE will be attached to a luxR to allow for the lux pathway to be shut off during the day and turn on during the night.



The Temperature Sensor

The temperature sensor will be cold sensitive that will cause the production of a repressor which will be used to repress one of the pathways (lux2). The repressed lux2 pathway carries another repressor for the lux1 pathway that is transcribed only when the temperature senor is turned off. This will allow for the change of color due to temperature.

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Our Team

Full Time

Part Time



The Lab Book



Our Sponsors



Special Thanks

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