Team:UNIPV-Pavia/Protocols/GelExtraction

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<h1>The protocols we used</h1>
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*[[Team:UNIPV-Pavia/Protocols/Lb|LB medium preparation]]
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*[[Team:UNIPV-Pavia/Protocols/Resuspension|Plasmid resuspension from IGEM paper spots]]
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*[[Team:UNIPV-Pavia/Protocols/Transformation|Transformation]]
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*[[Team:UNIPV-Pavia/Protocols/PlasmidExtraction|Plasmid extraction]]
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*[[Team:UNIPV-Pavia/Protocols/Digestion|BioBrick digestion with restriction enzymes]]
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*[[Team:UNIPV-Pavia/Protocols/GelExtraction|DNA gel extraction]]
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*[[Team:UNIPV-Pavia/Protocols/Precipitation|DNA precipitation with sodium acetate]]
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*[[Team:UNIPV-Pavia/Protocols/AntarcticPhosphatase|Antarctic Phosphatase]]
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*[[Team:UNIPV-Pavia/Protocols/Ligation|Ligation]]
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*[[Team:UNIPV-Pavia/Protocols/Pcr|PCR]]
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<h1>DNA gel extraction</h1>
<h1>DNA gel extraction</h1>
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''(Estimated time: 1 hour)''
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''(Estimated time: 1 hour and 30 min)''
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'''Materials needed:'''
'''Materials needed:'''
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*'''A gel in which the bands of interest can be seen'''
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*'''Agarose gel with well-separated, sharp bands'''
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*'''Scalpel and tweezers to cut gel'''
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*'''Scalpel and tweezers to cut gel''' (they have to be cleaned in ddH2O after every cut)
*'''Roche Agarose Gel DNA Extraction Kit'''
*'''Roche Agarose Gel DNA Extraction Kit'''
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*Follow Roche standard protocol: [https://www.roche-applied-science.com/pack-insert/1696505a.pdf Roche Agarose Gel DNA Extraction Kit protocol]
*Follow Roche standard protocol: [https://www.roche-applied-science.com/pack-insert/1696505a.pdf Roche Agarose Gel DNA Extraction Kit protocol]
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Latest revision as of 12:52, 1 July 2008

Home.jpg Home Unipv logo.jpg The Team And.jpg The Project Safety.jpg Biological Safety Dna.png Parts Submitted to the Registry
Laptop.jpg Dry Lab Pipette.jpg Wet Lab Math.gif Modeling Note.jpg Protocols Notebook.gif Activity Notebook


The protocols we used


DNA gel extraction

(Estimated time: 1 hour and 30 min)

Materials needed:

  • Agarose gel with well-separated, sharp bands
  • Scalpel and tweezers to cut gel (they have to be cleaned in ddH2O after every cut)
  • Roche Agarose Gel DNA Extraction Kit