"
Purdue/1 July 2008
From 2008.igem.org
(Difference between revisions)
(New page: Click Here to return to the notebook.) |
|||
| Line 1: | Line 1: | ||
[[Team:Purdue/Notebook | Click Here to return to the notebook.]] | [[Team:Purdue/Notebook | Click Here to return to the notebook.]] | ||
| + | |||
| + | ===Transformation: Take 3=== | ||
| + | *Using 2 spots of filter paper to try to get more DNA | ||
| + | *Using 16uL of TE instead of 10 (because the filter paper absorbs so much) | ||
| + | *After spinning it down at 15000xg for 3 min., we're letting it sit out overnight to try to get more DNA | ||
| + | *Tomorrow, we'll test how much DNA we have with a NanoDrop (more accurate and easy to use than the UV-Vis machine from earlier) | ||
| + | *We'll also test the transformation efficiency with the Invitrogen kit that comes with the cells (pUC17 stuff) | ||
| + | *Try to transform 2 parts: | ||
| + | **J09855 from 2008: it's from a DH5a strain, QC is great, and is on pSB1A2 plasmid | ||
| + | **Part from last year to compare filter paper w/ non-paper: J13003--it's a long part (like LacZ), on pSB1A2, and QC is great | ||
| + | |||
| + | |||
| + | '''Edited by Janie Stine''' | ||
Latest revision as of 18:25, 1 July 2008
Click Here to return to the notebook.
Transformation: Take 3
- Using 2 spots of filter paper to try to get more DNA
- Using 16uL of TE instead of 10 (because the filter paper absorbs so much)
- After spinning it down at 15000xg for 3 min., we're letting it sit out overnight to try to get more DNA
- Tomorrow, we'll test how much DNA we have with a NanoDrop (more accurate and easy to use than the UV-Vis machine from earlier)
- We'll also test the transformation efficiency with the Invitrogen kit that comes with the cells (pUC17 stuff)
- Try to transform 2 parts:
- J09855 from 2008: it's from a DH5a strain, QC is great, and is on pSB1A2 plasmid
- Part from last year to compare filter paper w/ non-paper: J13003--it's a long part (like LacZ), on pSB1A2, and QC is great
Edited by Janie Stine
