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Template:Team:UC Berkeley/Notebook/CC construction
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CC010 Reverse Biobricking of {<barnase!} | CC010 Reverse Biobricking of {<barnase!} | ||
CCAGTggatcCTTATCTGATTTTTG | CCAGTggatcCTTATCTGATTTTTG | ||
| + | </pre> | ||
| + | |||
| + | ==K112507== | ||
| + | <pre> | ||
| + | Construction of {<barnase!} basic part K112506 | ||
| + | |||
| + | PCR mea035/mea034 on Bacillus amyloliquefaciens gen. (320 bp, EcoRI/BamHI) | ||
| + | Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) | ||
| + | Product is pBca1256-K112507 | ||
| + | ---------------------------------------- | ||
| + | mea035 Forward Biobricking of | ||
| + | {rbs.barstar!} cgataGAATTCatgAGATCTcataagaaaggagccgcacatg | ||
| + | mea034 Reverse Biobricking of | ||
| + | {rbs.barstar!} cgttaGGATCCttaagaaagtatgatggtgatgtcgc | ||
</pre> | </pre> | ||
Revision as of 02:06, 2 July 2008
Contents |
K112500
Construction of {<HA>} basic part K112501
Wobble PCR of cc001/cc002 ( 50 bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI, 2227+ 910,L)
Product is pBca1256-K112500
--------------------------------------------
cc001 Forward Biobricking of {<HA>}
CgATAgaattcATGagatcttacccatacgacgtcccagac
cc002 Reverse biobricking of {<HA>}
ccagtggatccccagcgtagtctgggacgtcgtatggg
K112501
Construction of {<HA!} basic part K112502
Wobble PCR of cc001/cc003 ( 53 bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI, 2227+ 910,L)
Product is pBca1256-K112501
--------------------------------------------
cc001 Forward Biobricking of {<HA>}
CgATAgaattcATGagatcttacccatacgacgtcccagac
cc003 Reverse biobricking of {<HA!}
ccagtggatccttaccagcgtagtctgggacgtcgtatgggtaag
K112502
Construction of {<myc>} basic part K112503
Wobble PCR of cc004/cc005 ( 51 bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI, 2227+ 910,L)
Product is pBca1256-K112502
--------------------------------------------
cc004 Forward Biobricking of {<myc>}
CgATAgaattcATGagatctGAACAAAAACTCATCTCAGAAGAGG
cc005 Reverse Biobricking of {<myc>}
ccagtggatccCAGATCCTCTTCTGAGATGAGTTTTTG
K112503
Construction of {<myc!} basic part K112503
Wobble PCR of cc004/cc006 ( 54 bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI,2227+ 910,L)
Product is pBca1256-K112503
--------------------------------------------
cc004 Forward Biobricking of {<myc>}
CgATAgaattcATGagatctGAACAAAAACTCATCTCAGAAGAGG
cc006 Reverse Biobricking of {<myc!}
ccagtggatccTTACAGATCCTCTTCTGAGATGAGTTTTTGTTC
K112504
Construction of {<barnase>} basic part K112504
PCR CC007/CC008 on barnase gene ( 351 bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI, 2227+ 910,L)
Product is pBca1256-K112504
--------------------------------------------
cc007 Forward Biobricking of {<barnase>}
CGATAgaattcATGaGATCTGCACA
CC008 Reverse Biobricking of {<barnase>}
CCAGTggatcCTCTGATTTTTG
K112505
Construction of {barnase>} basic part K112505
PCR CC009/CC008 on barnase gene ( 354 bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI, 2227+ 910,L)
Product is pBca1256-K112505
--------------------------------------------
cc009 Forward Biobricking of {barnase>}
CGATAgaattcATGagatctATGGCACAGGTTATCAACACGTTTG
cc008 Reverse Biobricking of {barnase>}
CCAGTggatcCTCTGATTTTTG
K112506
Construction of {<barnase!} basic part K112506
PCR CC007/CC010 on barnase gene ( 354 bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI, 2227+ 910,L)
Product is pBca1256-K112506
--------------------------------------------
cc007 Forward Biobricking of {<barnase!}
CGATAgaattcATGaGATCTGCACA
CC010 Reverse Biobricking of {<barnase!}
CCAGTggatcCTTATCTGATTTTTG
K112507
Construction of {<barnase!} basic part K112506
PCR mea035/mea034 on Bacillus amyloliquefaciens gen. (320 bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112507
----------------------------------------
mea035 Forward Biobricking of
{rbs.barstar!} cgataGAATTCatgAGATCTcataagaaaggagccgcacatg
mea034 Reverse Biobricking of
{rbs.barstar!} cgttaGGATCCttaagaaagtatgatggtgatgtcgc
