Template:Team:UC Berkeley/Notebook/SC construction

From 2008.igem.org

(Difference between revisions)
Line 57: Line 57:
ccagtGGATCCttacttgtcgtcgtcatccttgtagtc
ccagtGGATCCttacttgtcgtcgtcatccttgtagtc
</pre>
</pre>
 +
 +
== pBca1256-K112604 ==
 +
<pre>
 +
Construction of {rbs_barstar>} basic part K112604    (320 bp, EcoRI/BamHI)
 +
PCR sc007/sc008 on Bacillus amyloliquefaciens gen.  (EcoRI/BamHI, 2472+910, L)         
 +
Sub into pBca1256                                           
 +
Product is pBca1256-K112604
 +
-------------------------------
 +
sc007    Fwd biobricking of {rbs_barstar>}
 +
cgataGAATTCatgAGATCTcataagaaaggagccgcacatg
 +
sc008    Reverse biobricking of {rbs_barstar>}
 +
cgttaGGATCCagaaagtatgatggtgatgtcgc 
 +
</pre>
 +
 +
== pBca1256-K112605 ==
 +
<pre>
 +
Construction of {prepro>} of PhoA basic part K112605      (114 bp, EcoRI/BamHI)
 +
CR sc009/sc010 on MG1655                                  (EcoRI/BamHI, 2472+910, L)
 +
Sub into pBca1256
 +
Product is pBca1256-K112605
 +
-------------------------------------------------------------
 +
sc009    Fwd biobricking of {prepro>}
 +
cgataGAATTCatgAGATCTgtacatggagaaaataaaatgaaacaaagc
 +
sc010    Reverse biobricking of {prepro>}
 +
cgtagGGATCCgggcttttgtcacaggggtaaac
 +
</pre>
 +
 +
 +
== pBca1256-K112606 ==
 +
<pre>
 +
Construction of {prepro>} of LamB basic part K112606          (132 bp, EcoRI/BamHI)
 +
PCR sc011/sc012 on MG1655                                      (EcoRI/BamHI, 2472+910, L)
 +
Sub into pBca1256
 +
Product is pBca1256-K112606
 +
-------------------------------------------------------------
 +
sc011    Fwd biobricking of {prepro>}
 +
cgataGAATTCatgAGATCTgaaaagcaatgactcaggagatag
 +
sc012    Reverse biobricking of {prepro>}
 +
cgtagGGATCCcagccattgcctgagcagacattac
 +
</pre>
 +
 +
 +
== pBca1256-K112607 ==
 +
<pre>
 +
Construction of {a~prepro>} of PhoA basic part K112607                  (100 bp, EcoRI/BamHI)
 +
PCR sc013/sc010                                                          (EcoRI/BamHI, 2472+910, L)
 +
Sub into pBca1256
 +
Product is pBca1256-K112607
 +
------------------------------------------------------
 +
sc013    Fwd biobricking of {a~prepro>}
 +
cgataGAATTCatgAGATCT taaa Atgaaacaaagcactattgcac
 +
sc010    Reverse biobricking of {a~prepro>}
 +
cgtagGGATCCgggcttttgtcacaggggtaaac
 +
</pre>
 +
 +
== pBca1256-K112608 ==
 +
<pre>
 +
Construction of {a~prepro>} of LamB basic part K112608          (111 bp, EcoRI/BamHI)
 +
PCR sc014/sc012                                                  (EcoRI/BamHI, 2472+910, L)
 +
Sub into pBca1256
 +
Product is pBca1256-K112608
 +
--------------------------------------------------
 +
sc014 Fwd biobricking of {a~prepro>}
 +
cgataGAATTCatgAGATCT taga atgatgattactctgc
 +
sc012 Reverse biobricking of {a~prepro>}
 +
cgtagGGATCCcagccattgcctgagcagacattac
 +

Revision as of 18:04, 2 July 2008

Contents


pBca1256-K112600 

Construction of <AP-tag> basic part K112600

Wobble PCR SC001/SC002  (66 bp, EcoRI/BamHI)
Sub into pBca1256           (EcoRI/BamHI, 2227+ 910)
Product is pBca1256-K112600
----------------------------------------------------
SC001F    Forward Biobricking of <AP-tag>
cgataGAATTCatgAGATCTggcctgaacgatatttttgaagcgcag
SC002R    Reverse Biobricking of <AP-tag>
ccagtGGATCCttcatgccattcaattttctgcgcttcaaaaatatcg

pBca1256-K112601 

Construction of <AP-tag! basic part K112601

Wobble PCR SC001/SC003 (69 bp, EcoRI/BamHI)
Sub into pBca1256           (EcoRI/BamHI, 2227+ 910)
Product is pBca1256-K112601
--------------------------------------------------
SC001     Forward Biobricking of <AP-tag!
cgataGAATTCatgAGATCTggcctgaacgatatttttgaagcgcag
SC003     Reverse Biobricking of <AP-tag!
ccagtGGATCCttattcatgccattcaattttctgcgcttcaaaaatatcg

pBca1256-K112602 

Construction of <FLAG-tag> basic part K112602

Wobble PCR SC004/SC005 (45 bp, EcoRI/BamHI)
Sub into pBca1256           (EcoRI/BamHI, 2227+ 910)
Product is pBca1256-K112602
-------------------------------------------------------------
SC004     Forward Biobricking of <FLAG-tag>
cgataGAATTCatgAGATCTgactacaaggatgacgacg
SC005     Reverse Biobricking of <FLAG-tag>
ccagtGGATCCcttgtcgtcgtcatccttgtagtc

pBca1256-K112603 

Construction of <FLAG-tag! basic part K112603

Wobble PCR SC004/SC006 (48 bp, EcoRI/BamHI)
Sub into pBca1256           (EcoRI/BamHI, 2227+ 910)
Product is pBca1256-K112603
-------------------------------------------------------------
SC004   Forward Biobricking of <FLAG-tag!
cgataGAATTCatgAGATCTgactacaaggatgacgacg
SC006   Reverse Biobricking of <FLAG-tag!
ccagtGGATCCttacttgtcgtcgtcatccttgtagtc

pBca1256-K112604 

Construction of {rbs_barstar>} basic part K112604    (320 bp, EcoRI/BamHI)
PCR sc007/sc008 on Bacillus amyloliquefaciens gen.   (EcoRI/BamHI, 2472+910, L)           
Sub into pBca1256                                             
Product is pBca1256-K112604
-------------------------------
sc007    Fwd biobricking of {rbs_barstar>}
cgataGAATTCatgAGATCTcataagaaaggagccgcacatg
sc008    Reverse biobricking of {rbs_barstar>}
cgttaGGATCCagaaagtatgatggtgatgtcgc

pBca1256-K112605 

Construction of {prepro>} of PhoA basic part K112605      (114 bp, EcoRI/BamHI)
CR sc009/sc010 on MG1655                                  (EcoRI/BamHI, 2472+910, L)
Sub into pBca1256
Product is pBca1256-K112605
-------------------------------------------------------------
sc009    Fwd biobricking of {prepro>}
cgataGAATTCatgAGATCTgtacatggagaaaataaaatgaaacaaagc
sc010    Reverse biobricking of {prepro>}
cgtagGGATCCgggcttttgtcacaggggtaaac


pBca1256-K112606 

Construction of {prepro>} of LamB basic part K112606           (132 bp, EcoRI/BamHI)
PCR sc011/sc012 on MG1655                                      (EcoRI/BamHI, 2472+910, L)
Sub into pBca1256
Product is pBca1256-K112606
-------------------------------------------------------------
sc011    Fwd biobricking of {prepro>}
cgataGAATTCatgAGATCTgaaaagcaatgactcaggagatag
sc012    Reverse biobricking of {prepro>}
cgtagGGATCCcagccattgcctgagcagacattac


pBca1256-K112607 

Construction of {a~prepro>} of PhoA basic part K112607                   (100 bp, EcoRI/BamHI)
PCR sc013/sc010                                                          (EcoRI/BamHI, 2472+910, L)
Sub into pBca1256
Product is pBca1256-K112607
------------------------------------------------------
sc013    Fwd biobricking of {a~prepro>}
cgataGAATTCatgAGATCT taaa Atgaaacaaagcactattgcac
sc010    Reverse biobricking of {a~prepro>}
cgtagGGATCCgggcttttgtcacaggggtaaac

pBca1256-K112608 

Construction of {a~prepro>} of LamB basic part K112608           (111 bp, EcoRI/BamHI)
PCR sc014/sc012                                                  (EcoRI/BamHI, 2472+910, L)
Sub into pBca1256
Product is pBca1256-K112608
--------------------------------------------------
sc014 Fwd biobricking of {a~prepro>}
cgataGAATTCatgAGATCT taga atgatgattactctgc
sc012 Reverse biobricking of {a~prepro>}
cgtagGGATCCcagccattgcctgagcagacattac




----
<div style="text-align: center;"> [[Team:UC_Berkeley/Notebook/Sherine_Cheung|Sherine Cheung]] </div>
<div style="text-align: center;"> [[Team:UC_Berkeley|Back to Berkeley Team Homepage]] </div>
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