Template:Team:UC Berkeley/Notebook/SC construction

From 2008.igem.org

(Difference between revisions)
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</pre>
</pre>
-
== pBca1256-K112604 (M) ==
+
== pBca1256-K112604 ==
<pre>
<pre>
-
Construction of {rbs_barstar>} basic part K112604   (320 bp, EcoRI/BamHI)
+
Construction of {rbs_barstar>} basic part K112604
-
PCR sc007/sc008 on Bacillus amyloliquefaciens gen.   (EcoRI/BamHI, 2472+910, L)          
+
PCR sc007/sc008 on Bacillus amyloliquefaciens gen.           (320 bp, EcoRI/BamHI)
-
Sub into pBca1256                                           
+
Sub into pBca1256                                            (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112604
Product is pBca1256-K112604
-------------------------------
-------------------------------
-
sc007    Fwd biobricking of {rbs_barstar>}
+
mea035  Fwd biobricking of {rbs_barstar>}  
-
cgataGAATTCatgAGATCTcataagaaaggagccgcacatg
+
cgataGAATTCatgAGATCTcataagaaaggagccgcacatg                            
-
sc008   Reverse biobricking of {rbs_barstar>}
+
sc007   Reverse biobricking of {rbs_barstar>}
cgttaGGATCCagaaagtatgatggtgatgtcgc   
cgttaGGATCCagaaagtatgatggtgatgtcgc   
 +
 
</pre>
</pre>
-
== pBca1256-K112605 (E) ==
+
== pBca1256-K112403 ==
<pre>
<pre>
-
Construction of {prepro>} of PhoA basic part K112605      (114 bp, EcoRI/BamHI)
+
Construction of {prepro>} of PhoA basic part K112403          (114 bp, EcoRI/BamHI)
-
CR sc009/sc010 on MG1655                                 (EcoRI/BamHI, 2472+910, L)
+
CR sc009/sc010 on MG1655                                             (EcoRI/BamHI, 2472+910, L)
Sub into pBca1256
Sub into pBca1256
-
Product is pBca1256-K112605
+
Product is pBca1256-K112403                                           
-------------------------------------------------------------
-------------------------------------------------------------
-
sc009    Fwd biobricking of {prepro>}
+
cb10007-phoA-F  Fwd biobricking of {prepro>}
cgataGAATTCatgAGATCTgtacatggagaaaataaaatgaaacaaagc
cgataGAATTCatgAGATCTgtacatggagaaaataaaatgaaacaaagc
-
sc010    Reverse biobricking of {prepro>}
+
cb10008-phoA-R  Reverse biobricking of {prepro>}
cgtagGGATCCgggcttttgtcacaggggtaaac
cgtagGGATCCgggcttttgtcacaggggtaaac
</pre>
</pre>
-
== pBca1256-K112606 (E) ==
+
== pBca1256-K112404  ==
<pre>
<pre>
-
Construction of {prepro>} of LamB basic part K112606          (132 bp, EcoRI/BamHI)
+
Construction of {prepro>} of LamB basic part K112404              (132 bp, EcoRI/BamHI)
-
PCR sc011/sc012 on MG1655                                     (EcoRI/BamHI, 2472+910, L)
+
PCR sc011/sc012 on MG1655                                         (EcoRI/BamHI, 2472+910, L)
-
Sub into pBca1256
+
Sub into pBca1256                                                              
-
Product is pBca1256-K112606
+
Product is pBca1256-K112404                                           
-------------------------------------------------------------
-------------------------------------------------------------
-
sc011    Fwd biobricking of {prepro>}
+
cb10009-lamB-F Fwd biobricking of {prepro>}
cgataGAATTCatgAGATCTgaaaagcaatgactcaggagatag
cgataGAATTCatgAGATCTgaaaagcaatgactcaggagatag
-
sc012    Reverse biobricking of {prepro>}
+
cb10010-lamB-R  Reverse biobricking of {prepro>}
cgtagGGATCCcagccattgcctgagcagacattac
cgtagGGATCCcagccattgcctgagcagacattac
 +
</pre>
</pre>
-
== pBca1256-K112607 ==
+
== pBca1256-K112605 ==
<pre>
<pre>
-
Construction of {a~prepro>} of PhoA basic part K112607                   (100 bp, EcoRI/BamHI)
+
Construction of {a~prepro>} of PhoA basic part K112605                   (100 bp, EcoRI/BamHI)
-
PCR sc013/sc010                                                          (EcoRI/BamHI, 2472+910, L)
+
PCR sc008/cb10008                                                                                (EcoRI/BamHI, 2472+910, L)
Sub into pBca1256
Sub into pBca1256
-
Product is pBca1256-K112607
+
Product is pBca1256-K112605
------------------------------------------------------
------------------------------------------------------
-
sc013    Fwd biobricking of {a~prepro>}
+
sc008 Fwd biobricking of {a~prepro>}
cgataGAATTCatgAGATCT taaa Atgaaacaaagcactattgcac
cgataGAATTCatgAGATCT taaa Atgaaacaaagcactattgcac
-
sc010    Reverse biobricking of {a~prepro>}
+
cb10008-phoA-R Reverse biobricking of {a~prepro>}
cgtagGGATCCgggcttttgtcacaggggtaaac
cgtagGGATCCgggcttttgtcacaggggtaaac
</pre>
</pre>
-
== pBca1256-K112608 ==
+
== pBca1256-K112606 ==
<pre>
<pre>
-
Construction of {a~prepro>} of LamB basic part K112608          (111 bp, EcoRI/BamHI)
+
Construction of {a~prepro>} of LamB basic part K112606                (111 bp, EcoRI/BamHI)
-
PCR sc014/sc012                                                  (EcoRI/BamHI, 2472+910, L)
+
PCR 009/cb10010                                                        (EcoRI/BamHI, 2472+910, L)
Sub into pBca1256
Sub into pBca1256
-
Product is pBca1256-K112608
+
Product is pBca1256-K112606
--------------------------------------------------
--------------------------------------------------
-
sc014  Fwd biobricking of {a~prepro>}
+
sc009 Fwd biobricking of {a~prepro>}
cgataGAATTCatgAGATCT taga atgatgattactctgc
cgataGAATTCatgAGATCT taga atgatgattactctgc
-
sc012  Reverse biobricking of {a~prepro>}
+
cb10010-lamB-R Reverse biobricking of {a~prepro>}
cgtagGGATCCcagccattgcctgagcagacattac
cgtagGGATCCcagccattgcctgagcagacattac
</pre>
</pre>
-
== pBca1256-K112609 ==
+
== pBca1256-K112607  ==
<pre>
<pre>
-
Construction of {rbs_prepro>} of PhoA basic part K112609               (113 bp, EcoRI/BamHI)
+
Construction of {rbs_prepro>} of PhoA basic part K112607               (113 bp, EcoRI/BamHI)
-
PCR sc015/sc010                                                       (EcoRI/BamHI, 2472+910, L)
+
PCR sc010/cb10008                                                      (EcoRI/BamHI, 2472+910, L)
Sub into pBca1256
Sub into pBca1256
-
Product is pBca1256-K112609
+
Product is pBca1256-K112607
------------------------------------------------------
------------------------------------------------------
-
sc015  Fwd biobricking of {rbs_prepro>}
+
sc010 Fwd biobricking of {rbs_prepro>}
cgataGAATTCatgAGATCT gtacatggagaaaataaaAtgaaacaaag
cgataGAATTCatgAGATCT gtacatggagaaaataaaAtgaaacaaag
-
sc010  Reverse biobricking of {rbs_prepro>}
+
cb10008-phoA-R  Reverse biobricking of {rbs_prepro>}
cgtagGGATCCgggcttttgtcacaggggtaaac
cgtagGGATCCgggcttttgtcacaggggtaaac
</pre>
</pre>
-
== pBca1256-K112610==
+
== pBca1256-K112608 ==
<pre>
<pre>
-
Construction of {rbs_prepro>} of LamB basic part K112610                  (126 bp, EcoRI/BamHI)
+
Construction of {rbs_prepro>} of LamB basic part K112608        (126 bp, EcoRI/BamHI)
-
PCR sc016/sc012                                                            (EcoRI/BamHI, 2472+910, L)
+
PCR sc011/cb10010                                                                                    (EcoRI/BamHI, 2472+910, L)
Sub into pBca1256
Sub into pBca1256
-
Product is pBca1256-K112610
+
Product is pBca1256-K112608
------------------------------------------------------
------------------------------------------------------
-
sc016 Fwd biobricking of {rbs_prepro>}
+
sc011 Fwd biobricking of {rbs_prepro>}
cgataGAATTCatgAGATCT caatgactcaggagataga atgatgattac
cgataGAATTCatgAGATCT caatgactcaggagataga atgatgattac
-
sc012 Reverse biobricking of {rbs_prepro>}
+
cb10010-lamB-R Reverse biobricking of {rbs_prepro>}
cgtagGGATCCcagccattgcctgagcagacattac
cgtagGGATCCcagccattgcctgagcagacattac
</pre>
</pre>

Revision as of 19:20, 2 July 2008

Contents


pBca1256-K112600 

Construction of <AP-tag> basic part K112600

Wobble PCR SC001/SC002  (66 bp, EcoRI/BamHI)
Sub into pBca1256           (EcoRI/BamHI, 2227+ 910)
Product is pBca1256-K112600
----------------------------------------------------
SC001F    Forward Biobricking of <AP-tag>
cgataGAATTCatgAGATCTggcctgaacgatatttttgaagcgcag
SC002R    Reverse Biobricking of <AP-tag>
ccagtGGATCCttcatgccattcaattttctgcgcttcaaaaatatcg

pBca1256-K112601 

Construction of <AP-tag! basic part K112601

Wobble PCR SC001/SC003 (69 bp, EcoRI/BamHI)
Sub into pBca1256           (EcoRI/BamHI, 2227+ 910)
Product is pBca1256-K112601
--------------------------------------------------
SC001     Forward Biobricking of <AP-tag!
cgataGAATTCatgAGATCTggcctgaacgatatttttgaagcgcag
SC003     Reverse Biobricking of <AP-tag!
ccagtGGATCCttattcatgccattcaattttctgcgcttcaaaaatatcg

pBca1256-K112602 

Construction of <FLAG-tag> basic part K112602

Wobble PCR SC004/SC005 (45 bp, EcoRI/BamHI)
Sub into pBca1256           (EcoRI/BamHI, 2227+ 910)
Product is pBca1256-K112602
-------------------------------------------------------------
SC004     Forward Biobricking of <FLAG-tag>
cgataGAATTCatgAGATCTgactacaaggatgacgacg
SC005     Reverse Biobricking of <FLAG-tag>
ccagtGGATCCcttgtcgtcgtcatccttgtagtc

pBca1256-K112603 

Construction of <FLAG-tag! basic part K112603

Wobble PCR SC004/SC006 (48 bp, EcoRI/BamHI)
Sub into pBca1256           (EcoRI/BamHI, 2227+ 910)
Product is pBca1256-K112603
-------------------------------------------------------------
SC004   Forward Biobricking of <FLAG-tag!
cgataGAATTCatgAGATCTgactacaaggatgacgacg
SC006   Reverse Biobricking of <FLAG-tag!
ccagtGGATCCttacttgtcgtcgtcatccttgtagtc

pBca1256-K112604 

Construction of {rbs_barstar>} basic part K112604
PCR sc007/sc008 on Bacillus amyloliquefaciens gen.           (320 bp, EcoRI/BamHI)
Sub into pBca1256                                            (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112604
-------------------------------
mea035   Fwd biobricking of {rbs_barstar>} 
cgataGAATTCatgAGATCTcataagaaaggagccgcacatg                              
sc007    Reverse biobricking of {rbs_barstar>}
cgttaGGATCCagaaagtatgatggtgatgtcgc

pBca1256-K112403 

Construction of {prepro>} of PhoA basic part K112403          (114 bp, EcoRI/BamHI)
CR sc009/sc010 on MG1655                                             (EcoRI/BamHI, 2472+910, L)
Sub into pBca1256
Product is pBca1256-K112403                                            
-------------------------------------------------------------
cb10007-phoA-F  Fwd biobricking of {prepro>}
cgataGAATTCatgAGATCTgtacatggagaaaataaaatgaaacaaagc
cb10008-phoA-R  Reverse biobricking of {prepro>}
cgtagGGATCCgggcttttgtcacaggggtaaac


pBca1256-K112404 

Construction of {prepro>} of LamB basic part K112404              (132 bp, EcoRI/BamHI)
PCR sc011/sc012 on MG1655                                         (EcoRI/BamHI, 2472+910, L)
Sub into pBca1256                                                               
Product is pBca1256-K112404                                            
-------------------------------------------------------------
cb10009-lamB-F Fwd biobricking of {prepro>}
cgataGAATTCatgAGATCTgaaaagcaatgactcaggagatag
cb10010-lamB-R   Reverse biobricking of {prepro>}
cgtagGGATCCcagccattgcctgagcagacattac


pBca1256-K112605 

Construction of {a~prepro>} of PhoA basic part K112605                   (100 bp, EcoRI/BamHI)
PCR sc008/cb10008                                                                                (EcoRI/BamHI, 2472+910, L)
Sub into pBca1256
Product is pBca1256-K112605
------------------------------------------------------
sc008 Fwd biobricking of {a~prepro>}
cgataGAATTCatgAGATCT taaa Atgaaacaaagcactattgcac
cb10008-phoA-R Reverse biobricking of {a~prepro>}
cgtagGGATCCgggcttttgtcacaggggtaaac

pBca1256-K112606 

Construction of {a~prepro>} of LamB basic part K112606                 (111 bp, EcoRI/BamHI)
PCR 009/cb10010                                                        (EcoRI/BamHI, 2472+910, L)
Sub into pBca1256
Product is pBca1256-K112606
--------------------------------------------------
sc009 Fwd biobricking of {a~prepro>}
cgataGAATTCatgAGATCT taga atgatgattactctgc
cb10010-lamB-R Reverse biobricking of {a~prepro>}
cgtagGGATCCcagccattgcctgagcagacattac

pBca1256-K112607 

Construction of {rbs_prepro>} of PhoA basic part K112607               (113 bp, EcoRI/BamHI)
PCR sc010/cb10008                                                      (EcoRI/BamHI, 2472+910, L)
Sub into pBca1256
Product is pBca1256-K112607
------------------------------------------------------
sc010 Fwd biobricking of {rbs_prepro>}
cgataGAATTCatgAGATCT gtacatggagaaaataaaAtgaaacaaag
cb10008-phoA-R  Reverse biobricking of {rbs_prepro>}
cgtagGGATCCgggcttttgtcacaggggtaaac

pBca1256-K112608 

Construction of {rbs_prepro>} of LamB basic part  K112608         (126 bp, EcoRI/BamHI)
PCR sc011/cb10010                                                                                    (EcoRI/BamHI, 2472+910, L)
Sub into pBca1256
Product is pBca1256-K112608
------------------------------------------------------
sc011 Fwd biobricking of {rbs_prepro>}
cgataGAATTCatgAGATCT caatgactcaggagataga atgatgattac
cb10010-lamB-R Reverse biobricking of {rbs_prepro>}
cgtagGGATCCcagccattgcctgagcagacattac


Sherine Cheung
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