Team:MIT/Notebook/Data/toothbinding/6-30

From 2008.igem.org

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[[Image:S._Mutans_plate_(6-30).jpg| 200px| S. Mutans plate (6-30)]]
[[Image:S._Mutans_plate_(6-30).jpg| 200px| S. Mutans plate (6-30)]]
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Centrifuged plated saliva
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Centrifuged plated saliva: day 2
[[Image:centsaliva.jpg| 200px| plated centrifuged saliva]]
[[Image:centsaliva.jpg| 200px| plated centrifuged saliva]]
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Uncentrifuged saliva
+
Centrifuged plated saliva: day 3
 +
[[Image:centsaliva2.jpg| 200px| plated centrifuged saliva]]
 +
 
 +
Uncentrifuged saliva: day 2
[[Image:dirtysaliva.jpg| 200px| plated uncentrifuged saliva]]
[[Image:dirtysaliva.jpg| 200px| plated uncentrifuged saliva]]
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Binding Assay (with HA beads)
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Uncentrifuged saliva: day 3
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[[Image:dirtysaliva2.jpg| 200px| plated uncentrifuged saliva]]
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 +
{| border="1" class="wikitable" style="text-align:center"
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|+Toothbinding assay key with HA (D=Diluted Saliva UD=Undiluted Saliva)
 +
|-
 +
! cells !!  5 min. !!  1h !!  2h  !!  5 min.    !!  1h !!  2h
 +
|-
 +
! 500
 +
| UD || UD || UD  | UD || D || D || D || D |
 +
|-
 +
! 167
 +
| - || - || -  | - || - || - || - |
 +
|-
 +
! 56
 +
| - || - || -  | - || - || - || - |
 +
|-
 +
! 18
 +
| - || - || -  | - || - || - || - |
 +
|-
 +
! 6
 +
| - || - || -  | - || - || - || - |
 +
|-
 +
! 2
 +
| - || - || -  | - || - || - || - |
 +
|}
 +
 
 +
Binding Assay (with HA beads):day 1
[[Image:HAbindingassay.jpg| 200px| Binding assay with HA]]
[[Image:HAbindingassay.jpg| 200px| Binding assay with HA]]
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Binding Assay (without HA)
+
 
 +
Binding Assay (with HA beads):day 2
 +
[[Image:HAassay2.jpg| 200px| Binding assay with HA]]
 +
 
 +
{| border="1" class="wikitable" style="text-align:center"
 +
|+Toothbinding assay key without HA (D=Diluted Saliva UD=Undiluted Saliva)
 +
|-
 +
! cells !!  5 min. !!  1h !!  2h  !!  5 min.    !!  1h !!  2h
 +
|-
 +
! 500
 +
| D || D || D  | D || UD || UD || UD || UD |
 +
|-
 +
! 167
 +
| - || - || -  | - || - || - || - |
 +
|-
 +
! 56
 +
| - || - || -  | - || - || - || - |
 +
|-
 +
! 18
 +
| - || - || -  | - || - || - || - |
 +
|-
 +
! 6
 +
| - || - || -  | - || - || - || - |
 +
|-
 +
! 2
 +
| - || - || -  | - || - || - || - |
 +
|}
 +
 
 +
Binding Assay (without HA): day 1
[[Image:NonHAbinding.jpg| 200px| Binding assay without HA]]
[[Image:NonHAbinding.jpg| 200px| Binding assay without HA]]
 +
 +
 +
Binding Assay (without HA): day 2
 +
[[Image:Sailvabinding2.jpg| 200px| Binding assay without HA]]

Latest revision as of 19:17, 3 July 2008

Plated S. Mutans S. Mutans plate (6-30)

Centrifuged plated saliva: day 2 plated centrifuged saliva

Centrifuged plated saliva: day 3 plated centrifuged saliva

Uncentrifuged saliva: day 2 plated uncentrifuged saliva

Uncentrifuged saliva: day 3 plated uncentrifuged saliva

Toothbinding assay key with HA (D=Diluted Saliva UD=Undiluted Saliva)
cells 5 min. 1h 2h 5 min. 1h 2h
500 UD UD UD D D D
167 - - - - -
56 - - - - -
18 - - - - -
6 - - - - -
2 - - - - -

Binding Assay (with HA beads):day 1 Binding assay with HA


Binding Assay (with HA beads):day 2 Binding assay with HA

Toothbinding assay key without HA (D=Diluted Saliva UD=Undiluted Saliva)
cells 5 min. 1h 2h 5 min. 1h 2h
500 D D D UD UD UD
167 - - - - -
56 - - - - -
18 - - - - -
6 - - - - -
2 - - - - -

Binding Assay (without HA): day 1 Binding assay without HA


Binding Assay (without HA): day 2 Binding assay without HA

Retrieved from "http://2008.igem.org/Team:MIT/Notebook/Data/toothbinding/6-30"