Team:UC Berkeley/Resources/MMCalculator

From 2008.igem.org

(Difference between revisions)
 
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Line 40: Line 40:
case 3:
case 3:
out.value += "Colony PCR Master Mix for " + num + " reactions:\n";
out.value += "Colony PCR Master Mix for " + num + " reactions:\n";
-
out.value += "Water: " + (15.95*num) + "\n";
+
out.value += "Water: " + (16*num) + "\n";
out.value += "MgCl (50 mM): " + (.6*num) + "\n";
out.value += "MgCl (50 mM): " + (.6*num) + "\n";
out.value += "10 mM dNTP: " + (.4*num) + "\n";
out.value += "10 mM dNTP: " + (.4*num) + "\n";
out.value += "10x Taq Buffer: " + (2*num) + "\n";
out.value += "10x Taq Buffer: " + (2*num) + "\n";
-
out.value += "Taq Polymerase: " + (.25*num) + "\n";
+
out.value += "Taq Polymerase: " + (.2*num) + "\n";
out.value += "ca998 Oligo(10 mM): " + (.4*num) + "\n";
out.value += "ca998 Oligo(10 mM): " + (.4*num) + "\n";
out.value += "g00101 Oligo(10 mM): " + (.4*num) + "\n";
out.value += "g00101 Oligo(10 mM): " + (.4*num) + "\n";
Line 67: Line 67:
break;
break;
case 6:
case 6:
-
out.value += "Regular PCR w/o DMSO, 50 uL Scale Master Mix for " + num + " reactions:\n";
+
out.value += "Regular PCR w/o DMSO w/ oligos, 50 uL Scale Master Mix for " + num + " reactions:\n";
-
out.value += "Water: " + (43*num) + "\n";
+
out.value += "Water: " + (40.75*num) + "\n";
out.value += "10 mM dNTP: " + (1*num) + "\n";
out.value += "10 mM dNTP: " + (1*num) + "\n";
out.value += "10x Expand Buffer \"2\": " + (5*num) + "\n";
out.value += "10x Expand Buffer \"2\": " + (5*num) + "\n";
 +
out.value += "Oligo #1: " + (1*num) + "\n";
 +
out.value += "Oligo #2: " + (1*num) + "\n";
out.value += "Expand Polymerase \"1\": " + (.75*num) + "\n";
out.value += "Expand Polymerase \"1\": " + (.75*num) + "\n";
-
out.value += "Aliquot 49.75 uL\n";
+
out.value += "Aliquot 49.5 uL\n";
break;
break;
case 7:
case 7:
-
out.value += "Basic Part Plasmid Digestion Master Mix for " + num + " reactions:\n";
+
out.value += "Basic Part Transfer Digestion Master Mix for " + num + " reactions:\n";
out.value += "Water: " + (21*num) + "\n";
out.value += "Water: " + (21*num) + "\n";
-
out.value += "NEB2 Buffer: " + (3*num) + "\n";
+
out.value += "NEB3 Buffer: " + (3*num) + "\n";
out.value += "EcoRI: " + (.5*num) + "\n";
out.value += "EcoRI: " + (.5*num) + "\n";
out.value += "BamHI: " + (.5*num) + "\n";
out.value += "BamHI: " + (.5*num) + "\n";
-
out.value += "Aliquot 49.75 uL\n";
+
out.value += "Aliquot 25 uL\n";
 +
break;
 +
case 8:
 +
out.value += "Lefty Assembly Digestion Master Mix for " + num + " reactions:\n";
 +
out.value += "Water: " + (12*num) + "\n";
 +
out.value += "NEB2 Buffer: " + (2*num) + "\n";
 +
out.value += "BamHI: " + (.5*num) + "\n";
 +
out.value += "XhoI: " + (.5*num) + "\n";
 +
out.value += "Aliquot 15 uL\n";
 +
break;
 +
case 9:
 +
out.value += "Righty Assembly Digestion Master Mix for " + num + " reactions:\n";
 +
out.value += "Water: " + (12*num) + "\n";
 +
out.value += "NEB2 Buffer: " + (2*num) + "\n";
 +
out.value += "BglII: " + (.5*num) + "\n";
 +
out.value += "XhoI: " + (.5*num) + "\n";
 +
out.value += "Aliquot 15 uL\n";
break;
break;
}
}
Line 98: Line 116:
<option>Ligation</option>
<option>Ligation</option>
<option>Regular PCR w/o DMSO, 50 uL scale</option>
<option>Regular PCR w/o DMSO, 50 uL scale</option>
-
<option>Basic Part Plasmid Digest</option>
+
<option>Basic Part Transfer Digest</option>
 +
<option>Lefty Assembly Digest</option>
 +
<option>Righty Assembly Digest</option>
</select>
</select>
<br>
<br>

Latest revision as of 20:21, 6 July 2008

Bing's Master Mix Calcualtor Version 0.2a

All volumes are given in uL. The calculator will give you enough master mix for one extra reaction.

Select Reaction:

Number of Reactions:


Back to UCB Home
Retrieved from "http://2008.igem.org/Team:UC_Berkeley/Resources/MMCalculator"