Team:UC Berkeley/Resources/MMCalculator

From 2008.igem.org

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==Bing's Master Mix Calcualtor Version 0.1a==
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==Bing's Master Mix Calcualtor Version 0.2a==
'''All volumes are given in uL. The calculator will give you enough master mix for one extra reaction.'''
'''All volumes are given in uL. The calculator will give you enough master mix for one extra reaction.'''
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case 3:
case 3:
out.value += "Colony PCR Master Mix for " + num + " reactions:\n";
out.value += "Colony PCR Master Mix for " + num + " reactions:\n";
-
out.value += "Water: " + (14.4*num) + "\n";
+
out.value += "Water: " + (16*num) + "\n";
-
out.value += "MgCl (10 mM): " + (.6*num) + "\n";
+
out.value += "MgCl (50 mM): " + (.6*num) + "\n";
-
out.value += "10 mM dNTP: " + (2*num) + "\n";
+
out.value += "10 mM dNTP: " + (.4*num) + "\n";
out.value += "10x Taq Buffer: " + (2*num) + "\n";
out.value += "10x Taq Buffer: " + (2*num) + "\n";
out.value += "Taq Polymerase: " + (.2*num) + "\n";
out.value += "Taq Polymerase: " + (.2*num) + "\n";
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break;
break;
case 6:
case 6:
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out.value += "Regular PCR w/o DMSO, 50 uL Scale Master Mix for " + num + " reactions:\n";
+
out.value += "Regular PCR w/o DMSO w/ oligos, 50 uL Scale Master Mix for " + num + " reactions:\n";
-
out.value += "Water: " + (43*num) + "\n";
+
out.value += "Water: " + (40.75*num) + "\n";
out.value += "10 mM dNTP: " + (1*num) + "\n";
out.value += "10 mM dNTP: " + (1*num) + "\n";
out.value += "10x Expand Buffer \"2\": " + (5*num) + "\n";
out.value += "10x Expand Buffer \"2\": " + (5*num) + "\n";
 +
out.value += "Oligo #1: " + (1*num) + "\n";
 +
out.value += "Oligo #2: " + (1*num) + "\n";
out.value += "Expand Polymerase \"1\": " + (.75*num) + "\n";
out.value += "Expand Polymerase \"1\": " + (.75*num) + "\n";
-
out.value += "Aliquot 49.75 uL\n";
+
out.value += "Aliquot 49.5 uL\n";
 +
break;
 +
case 7:
 +
out.value += "Basic Part Transfer Digestion Master Mix for " + num + " reactions:\n";
 +
out.value += "Water: " + (21*num) + "\n";
 +
out.value += "NEB3 Buffer: " + (3*num) + "\n";
 +
out.value += "EcoRI: " + (.5*num) + "\n";
 +
out.value += "BamHI: " + (.5*num) + "\n";
 +
out.value += "Aliquot 25 uL\n";
 +
break;
 +
case 8:
 +
out.value += "Lefty Assembly Digestion Master Mix for " + num + " reactions:\n";
 +
out.value += "Water: " + (12*num) + "\n";
 +
out.value += "NEB2 Buffer: " + (2*num) + "\n";
 +
out.value += "BamHI: " + (.5*num) + "\n";
 +
out.value += "XhoI: " + (.5*num) + "\n";
 +
out.value += "Aliquot 15 uL\n";
 +
break;
 +
case 9:
 +
out.value += "Righty Assembly Digestion Master Mix for " + num + " reactions:\n";
 +
out.value += "Water: " + (12*num) + "\n";
 +
out.value += "NEB2 Buffer: " + (2*num) + "\n";
 +
out.value += "BglII: " + (.5*num) + "\n";
 +
out.value += "XhoI: " + (.5*num) + "\n";
 +
out.value += "Aliquot 15 uL\n";
break;
break;
}
}
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<option>Ligation</option>
<option>Ligation</option>
<option>Regular PCR w/o DMSO, 50 uL scale</option>
<option>Regular PCR w/o DMSO, 50 uL scale</option>
 +
<option>Basic Part Transfer Digest</option>
 +
<option>Lefty Assembly Digest</option>
 +
<option>Righty Assembly Digest</option>
</select>
</select>
<br>
<br>

Latest revision as of 20:21, 6 July 2008

Bing's Master Mix Calcualtor Version 0.2a

All volumes are given in uL. The calculator will give you enough master mix for one extra reaction.

Select Reaction:

Number of Reactions:


Back to UCB Home
Retrieved from "http://2008.igem.org/Team:UC_Berkeley/Resources/MMCalculator"