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Template:Team:UC Berkeley/Notebook/SC construction
From 2008.igem.org
(Difference between revisions)
| Line 6: | Line 6: | ||
Construction of <AP-tag> basic part K112600 | Construction of <AP-tag> basic part K112600 | ||
| - | Wobble PCR SC001/SC002 | + | Wobble PCR SC001/SC002 (66 bp, EcoRI/BamHI) |
| - | Sub into pBca1256 | + | Sub into pBca1256 (EcoRI/BamHI, 2227+ 910) |
Product is pBca1256-K112600 | Product is pBca1256-K112600 | ||
---------------------------------------------------- | ---------------------------------------------------- | ||
| Line 20: | Line 20: | ||
Construction of <AP-tag! basic part K112601 | Construction of <AP-tag! basic part K112601 | ||
| - | Wobble PCR SC001/SC003 (69 bp, EcoRI/BamHI) | + | Wobble PCR SC001/SC003 (69 bp, EcoRI/BamHI) |
| - | Sub into pBca1256 | + | Sub into pBca1256 (EcoRI/BamHI, 2227+ 910) |
Product is pBca1256-K112601 | Product is pBca1256-K112601 | ||
-------------------------------------------------- | -------------------------------------------------- | ||
| Line 34: | Line 34: | ||
Construction of <FLAG-tag> basic part K112602 | Construction of <FLAG-tag> basic part K112602 | ||
| - | Wobble PCR SC004/SC005 (45 bp, EcoRI/BamHI) | + | Wobble PCR SC004/SC005 (45 bp, EcoRI/BamHI) |
| - | Sub into pBca1256 | + | Sub into pBca1256 (EcoRI/BamHI, 2227+ 910) |
Product is pBca1256-K112602 | Product is pBca1256-K112602 | ||
| - | + | --------------------------------------------------- | |
SC004 Forward Biobricking of <FLAG-tag> | SC004 Forward Biobricking of <FLAG-tag> | ||
cgataGAATTCatgAGATCTgactacaaggatgacgacg | cgataGAATTCatgAGATCTgactacaaggatgacgacg | ||
| Line 48: | Line 48: | ||
Construction of <FLAG-tag! basic part K112603 | Construction of <FLAG-tag! basic part K112603 | ||
| - | Wobble PCR SC004/SC006 (48 bp, EcoRI/BamHI) | + | Wobble PCR SC004/SC006 (48 bp, EcoRI/BamHI) |
| - | Sub into pBca1256 | + | Sub into pBca1256 (EcoRI/BamHI, 2227+ 910) |
Product is pBca1256-K112603 | Product is pBca1256-K112603 | ||
| - | + | ----------------------------------------------------- | |
SC004 Forward Biobricking of <FLAG-tag! | SC004 Forward Biobricking of <FLAG-tag! | ||
cgataGAATTCatgAGATCTgactacaaggatgacgacg | cgataGAATTCatgAGATCTgactacaaggatgacgacg | ||
| Line 74: | Line 74: | ||
<pre> | <pre> | ||
Construction of {prepro>} of PhoA basic part K112403 (95 bp, EcoRI/BamHI) | Construction of {prepro>} of PhoA basic part K112403 (95 bp, EcoRI/BamHI) | ||
| - | CR cb10007/cb10008 on MG1655 | + | CR cb10007/cb10008 on MG1655 (EcoRI/BamHI, 2472+910, L) |
Sub into pBca1256 | Sub into pBca1256 | ||
Product is pBca1256-K112403 | Product is pBca1256-K112403 | ||
| Line 139: | Line 139: | ||
<pre> | <pre> | ||
Construction of {rbs_prepro>} of LamB basic part K112608 (126 bp, EcoRI/BamHI) | Construction of {rbs_prepro>} of LamB basic part K112608 (126 bp, EcoRI/BamHI) | ||
| - | PCR sc011/cb10010-lamB-R on E.coli K12 MG1655 | + | PCR sc011/cb10010-lamB-R on E.coli K12 MG1655 (EcoRI/BamHI, 2472+910, L) |
Sub into pBjh1601AK | Sub into pBjh1601AK | ||
Product is pBca1256-K112608 | Product is pBca1256-K112608 | ||
Revision as of 18:13, 7 July 2008
Contents |
pBca1256-K112600
Construction of <AP-tag> basic part K112600 Wobble PCR SC001/SC002 (66 bp, EcoRI/BamHI) Sub into pBca1256 (EcoRI/BamHI, 2227+ 910) Product is pBca1256-K112600 ---------------------------------------------------- SC001F Forward Biobricking of <AP-tag> cgataGAATTCatgAGATCTggcctgaacgatatttttgaagcgcag SC002R Reverse Biobricking of <AP-tag> ccagtGGATCCttcatgccattcaattttctgcgcttcaaaaatatcg
pBca1256-K112601
Construction of <AP-tag! basic part K112601 Wobble PCR SC001/SC003 (69 bp, EcoRI/BamHI) Sub into pBca1256 (EcoRI/BamHI, 2227+ 910) Product is pBca1256-K112601 -------------------------------------------------- SC001 Forward Biobricking of <AP-tag! cgataGAATTCatgAGATCTggcctgaacgatatttttgaagcgcag SC003 Reverse Biobricking of <AP-tag! ccagtGGATCCttattcatgccattcaattttctgcgcttcaaaaatatcg
pBca1256-K112602
Construction of <FLAG-tag> basic part K112602 Wobble PCR SC004/SC005 (45 bp, EcoRI/BamHI) Sub into pBca1256 (EcoRI/BamHI, 2227+ 910) Product is pBca1256-K112602 --------------------------------------------------- SC004 Forward Biobricking of <FLAG-tag> cgataGAATTCatgAGATCTgactacaaggatgacgacg SC005 Reverse Biobricking of <FLAG-tag> ccagtGGATCCcttgtcgtcgtcatccttgtagtc
pBca1256-K112603
Construction of <FLAG-tag! basic part K112603 Wobble PCR SC004/SC006 (48 bp, EcoRI/BamHI) Sub into pBca1256 (EcoRI/BamHI, 2227+ 910) Product is pBca1256-K112603 ----------------------------------------------------- SC004 Forward Biobricking of <FLAG-tag! cgataGAATTCatgAGATCTgactacaaggatgacgacg SC006 Reverse Biobricking of <FLAG-tag! ccagtGGATCCttacttgtcgtcgtcatccttgtagtc
pBca1256-K112604
Construction of {rbs_barstar>} basic part K112604
PCR mea035/sc007 on Bacillus amyloliquefaciens gen. (320 bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112604
-------------------------------
mea035 Fwd biobricking of {rbs_barstar>}
cgataGAATTCatgAGATCTcataagaaaggagccgcacatg
sc007 Reverse biobricking of {rbs_barstar>}
cgttaGGATCCagaaagtatgatggtgatgtcgc
pBca1256-K112403
Construction of {prepro>} of PhoA basic part K112403 (95 bp, EcoRI/BamHI)
CR cb10007/cb10008 on MG1655 (EcoRI/BamHI, 2472+910, L)
Sub into pBca1256
Product is pBca1256-K112403
-------------------------------------------------------------
cb10007-phoA-F Fwd biobricking of {prepro>}
cgataGAATTCatgAGATCTatgaaacaaagcactattgcactggc
cb10008-phoA-R Reverse biobricking of {prepro>}
cgtagGGATCCgggcttttgtcacaggggtaaac
pBca1256-K112404
Construction of {prepro>} of LamB basic part K112404 (107 bp, EcoRI/BamHI)
PCR cb10009/cb10010 on MG1655 (EcoRI/BamHI, 2472+910, L)
Sub into pBca1256
Product is pBca1256-K112404
-------------------------------------------------------------
cb10009-lamB-F Fwd biobricking of {prepro>}
cgataGAATTCatgAGATCTatgatgattactctgcgcaaac
cb10010-lamB-R Reverse biobricking of {prepro>}
cgtagGGATCCcagccattgcctgagcagacattac
pBca1256-K112605
Construction of {a~prepro>} of PhoA basic part K112605 (100 bp, EcoRI/BamHI)
PCR sc008/cb10008-phoA-R (EcoRI/BamHI, 2472+910, L)
Sub into pBca1256
Product is pBca1256-K112605
------------------------------------------------------
sc008 Fwd biobricking of {a~prepro>}
cgataGAATTCatgAGATCTtaaagtgaaacaaagcactattgcactggc
cb10008-phoA-R Reverse biobricking of {a~prepro>}
cgtagGGATCCgggcttttgtcacaggggtaaac
pBca1256-K112606
Construction of {a~prepro>} of LamB basic part K112606 (111 bp, EcoRI/BamHI)
PCR 009/cb10010-lamB-R (EcoRI/BamHI, 2472+910, L)
Sub into pBca1256
Product is pBca1256- K112606
--------------------------------------------------
sc009 Fwd biobricking of {a~prepro>}
cgataGAATTCatgAGATCTtagaatgatgattactctgcgc
cb10010-lamB-R Reverse biobricking of {a~prepro>}
cgtagGGATCCcagccattgcctgagcagacattac
pBca1256-K112607
Construction of {rbs_prepro>} of PhoA basic part K112607 (113 bp, EcoRI/BamHI)
PCR sc010/cb10008-PhoA-R (EcoRI/BamHI, 2472+910, L)
Sub into pBca1256
Product is pBca1256-K112607
------------------------------------------------------
sc010 Fwd biobricking of {rbs_prepro>}
cgataGAATTCatgAGATCTgtacatggagaaaataaaAtgaaacaaagcac
cb10008-phoA-R Reverse biobricking of {rbs_prepro>}
cgtagGGATCCgggcttttgtcacaggggtaaac
pBca1256-K112608
Construction of {rbs_prepro>} of LamB basic part K112608 (126 bp, EcoRI/BamHI)
PCR sc011/cb10010-lamB-R on E.coli K12 MG1655 (EcoRI/BamHI, 2472+910, L)
Sub into pBjh1601AK
Product is pBca1256-K112608
------------------------------------------------------
sc011 Fwd biobricking of {rbs_prepro>}
cgataGAATTCatgAGATCTcaatgactcaggagatagaatg
cb10010-lamB-R Reverse biobricking of {rbs_prepro>}
cgtagGGATCCcagccattgcctgagcagacattac
