"
Team:ESBS-Strasbourg/10 July 2008
From 2008.igem.org
(Difference between revisions)
(→WetLab) |
|||
| Line 6: | Line 6: | ||
== WetLab == | == WetLab == | ||
| + | *Katja: | ||
| + | ** Preparation of LB liquid media and LB(A) plates | ||
| + | ** Transformation (db3.1) with positive and negative control and BB_p1010 in Amipicillin and in Kanamycin backround | ||
* Sandra: | * Sandra: | ||
** resuspend primer to stock solutions of 50 µM | ** resuspend primer to stock solutions of 50 µM | ||
Revision as of 07:24, 11 July 2008
Contents |
DryLab
- MM: Sponsoring
Modeling
WetLab
- Katja:
- Preparation of LB liquid media and LB(A) plates
- Transformation (db3.1) with positive and negative control and BB_p1010 in Amipicillin and in Kanamycin backround
- Sandra:
- resuspend primer to stock solutions of 50 µM
- making primer aliquots of 10 µM (4x 50 µl)
- transformation (dh5alpha cells) with positiv and negative control and BioBrick GFP-device
- MM:
- TSB-Buffer
- Made competent cells (TOP10, db3.1, dh5alpha)
- Transformation (TOP10)
General
Quote of the day
MW: "I always got heavy beard growth -roarr"
