Team:ESBS-Strasbourg/11 July 2008

(Difference between revisions)

Revision as of 16:10, 12 July 2008

results SD, MM and KK:
- TOP10: 5* 10^5 cfu/ug
- db3.1: 10^5 cfu/ug
- dh5alpha: 10^6 cfu/ug

Primer
- the arrived verification primer were resuspended to give a stock solution of 50 µM
- aliquots of these primers (4x 10 µM) were produced and everything frozen (-20°C)

Transformation
- competent Top10 cells from Invitrogen, protocol from Marias Lab: H2O (negative control), pxj (positive control), 5 BioBricks (EYFP, vector without insert, adh terminator,...) {complete on monday; sd}, plated on ampicillin
- competent db3.1 cells, protocol from Barbaras Lab, DNA was already in KCM and cells were just added and plated, K3 on kanamycin and A2 on ampicillin
- competent dh5alpha cells, protocol from iGEM using SOC medium: H2O (negative control), pxj (positive control), BioBrick (EYFP), plated on ampicillin

"At least we have worked steril." (Marius)

@@ Line 9: / Line 9: @@
 ** db3.1: 10^5 cfu/ug
 ** dh5alpha: 10^6 cfu/ug
+<br>
+* Primer
+** the arrived verification primer were resuspended to give a stock solution of 50 µM
+** aliquots of these primers (4x 10 µM) were produced and everything frozen (-20°C)
+<br>
+* Transformation
+** competent Top10 cells from Invitrogen, protocol from Marias Lab: H2O (negative control), pxj (positive control), 5 BioBricks (EYFP, vector without insert, adh terminator,...) {complete on monday; sd}, plated on ampicillin
+** competent db3.1 cells, protocol from Barbaras Lab, DNA was already in KCM and cells were just added and plated, K3 on kanamycin and A2 on ampicillin
+** competent dh5alpha cells, protocol from iGEM using SOC medium: H2O (negative control), pxj (positive control), BioBrick (EYFP), plated on ampicillin
 == General ==