"
Team:ESBS-Strasbourg/Protocols
From 2008.igem.org
(Difference between revisions)
(→E.Coli) |
(→Cloning) |
||
| Line 8: | Line 8: | ||
==Cloning== | ==Cloning== | ||
| - | [[PCR]] <br> | + | [[Team:ESBS-Strasbourg/PCR|PCR]] <br> |
| - | [[Purification of PCR product]] <br> | + | [[Team:ESBS-Strasbourg/Purification of PCR product|Purification of PCR product]] <br> |
| - | [[Miniprep: plasmid amplification]] <br> | + | [[Team:ESBS-Strasbourg/Miniprep: plasmid amplification|Miniprep:plasmid amplification]] <br> |
| - | [[Plasmid | + | [[Team:ESBS-Strasbourg/Plasmid urification|Plasmid purification]] <br> |
| - | [[Restriction digestion]] <br> | + | [[Team:ESBS-Strasbourg/Restriction digestion|Restriction digestion]] <br> |
| - | [[Agarose gel preparation]] <br> | + | [[Team:ESBS-Strasbourg/Agarose gel preparation|Agarose gel preparation]] <br> |
| - | [[Dephosphorylation]] <br> | + | [[Team:ESBS-Strasbourg/Dephosphorylation|Dephosphorylation]] <br> |
| - | [[Ligation]] <br> | + | [[Team:ESBS-Strasbourg/Ligation|Ligation]] <br> |
==Solutions and buffers== | ==Solutions and buffers== | ||
'''SOB''' for the preparation of competent cells [http://openwetware.org/wiki/SOB SOB] <br> | '''SOB''' for the preparation of competent cells [http://openwetware.org/wiki/SOB SOB] <br> | ||
Revision as of 12:30, 14 July 2008
E.Coli
Preparation of competent cells (Mariels/Barbaras Protocol) Competent Bacteria
Preparation of TOP10 chemically competent cells [http://openwetware.org/wiki/TOP10_chemically_competent_cells igem standard protocol]
Transformation of competent cells (Mariels/Barbaras Protocol) Transformation
Cloning
PCR
Purification of PCR product
Miniprep:plasmid amplification
Plasmid purification
Restriction digestion
Agarose gel preparation
Dephosphorylation
Ligation
Solutions and buffers
SOB for the preparation of competent cells [http://openwetware.org/wiki/SOB SOB]
