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Team:ESBS-Strasbourg/Miniprep: plasmid amplification
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(New page: 1)Remarks <br> {| class="prettytable" style="width:50%; height:50px" border="1" cellspacing="0" |- class="hintergrundfarbe5" |- | '''Antibiotic''' || '''Working concentration''' |- | ...) |
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-Take one colony and put it in 5 to 7mL of LB medium containing the antibiotic <br> | -Take one colony and put it in 5 to 7mL of LB medium containing the antibiotic <br> | ||
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Revision as of 14:24, 14 July 2008
a) Remarks
| Antibiotic | Working concentration |
| Ampicillin | 50-100µg/mL |
| Chloramphenicol | 25-170µg/mL |
| Kanamycin | 10-50µg/mL |
| Streptomycin | 10-50µg/mL |
| Tetracyclin | 10-50µg/mL |
b) Protocol
-Take one colony and put it in 5 to 7mL of LB medium containing the antibiotic
-12 to 16h over night at 37°C, 200-250rpm
Notice that you will need about 5mL of culture to perform the plasmid purification (3mL of you do Plasmid quick pure purification)
