Team:ESBS-Strasbourg/11 July 2008

(Difference between revisions)

Revision as of 15:06, 14 July 2008

results SD, MM and KK:
- TOP10: 5* 10^5 cfu/ug
- db3.1: 10^5 cfu/ug
- dh5alpha: 10^6 cfu/ug

Primer
- the arrived verification primer were resuspended to give a stock solution of 50 µM
- aliquots of these primers (4x 10 µM) were produced and everything frozen (-20°C)

Transformation
- competent Top10 cells from Invitrogen, protocol from Marias Lab: H2O (negative control), pxj (positive control), 5 BioBricks (EYFP, vector without insert, adh terminator, lacI, lamdba CI), plated on ampicillin
- competent db3.1 cells, protocol from Barbaras Lab, DNA was already in KCM and cells were just added and plated, K3 on kanamycin and A2 on ampicillin
- competent dh5alpha cells, protocol from iGEM using SOC medium: H2O (negative control), pxj (positive control), BioBrick (EYFP), plated on ampicillin

"At least we have worked steril." (Marius)

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 <br>
 * Transformation
-** competent Top10 cells from Invitrogen, protocol from Marias Lab: H2O (negative control), pxj (positive control), 5 BioBricks (EYFP, vector without insert, adh terminator,...) {complete on monday; sd}, plated on ampicillin
+** competent Top10 cells from Invitrogen, protocol from Marias Lab: H2O (negative control), pxj (positive control), 5 BioBricks (EYFP, vector without insert, adh terminator, lacI, lamdba CI), plated on ampicillin
 ** competent db3.1 cells, protocol from Barbaras Lab, DNA was already in KCM and cells were just added and plated, K3 on kanamycin and A2 on ampicillin
 ** competent dh5alpha cells, protocol from iGEM using SOC medium: H2O (negative control), pxj (positive control), BioBrick (EYFP), plated on ampicillin