"
Team:ESBS-Strasbourg/Protocols
From 2008.igem.org
(Difference between revisions)
(→Cloning) |
(→E.Coli) |
||
| Line 3: | Line 3: | ||
'''Preparation of competent cells (Mariels/Barbaras Protocol)''' [[Team:ESBS-Strasbourg/Competent Bacteria | Competent Bacteria]] <br> | '''Preparation of competent cells (Mariels/Barbaras Protocol)''' [[Team:ESBS-Strasbourg/Competent Bacteria | Competent Bacteria]] <br> | ||
| - | '''Preparation of TOP10 chemically competent cells''' [http://openwetware.org/wiki/TOP10_chemically_competent_cells | + | '''Preparation of TOP10 chemically competent cells''' [http://openwetware.org/wiki/TOP10_chemically_competent_cells Igem Standard Protocol] <br> |
'''Transformation of competent cells (Mariels/Barbaras Protocol)''' [[Team:ESBS-Strasbourg/Transformation Barbara|Transformation]]<br> | '''Transformation of competent cells (Mariels/Barbaras Protocol)''' [[Team:ESBS-Strasbourg/Transformation Barbara|Transformation]]<br> | ||
Revision as of 22:25, 14 July 2008
E.Coli
Preparation of competent cells (Mariels/Barbaras Protocol) Competent Bacteria
Preparation of TOP10 chemically competent cells [http://openwetware.org/wiki/TOP10_chemically_competent_cells Igem Standard Protocol]
Transformation of competent cells (Mariels/Barbaras Protocol) Transformation
Cloning
PCR
Purification of PCR product
Miniprep:plasmid amplification
Plasmid purification
Restriction digestion
Agarose gel preparation
Dephosphorylation
Ligation
Solutions and buffers
SOB for the preparation of competent cells [http://openwetware.org/wiki/SOB SOB]
