Team:ESBS-Strasbourg/Protocols

From 2008.igem.org

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(E.Coli)
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==E.Coli==
==E.Coli==
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'''Preparation of competent cells (Mariels/Barbaras Protocol)''' [[Team:ESBS-Strasbourg/Competent Bacteria | Competent Bacteria]] <br>
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*'''Preparation of competent cells (Mariels/Barbaras Protocol)''' [[Team:ESBS-Strasbourg/Competent Bacteria | Competent Bacteria]] <br>
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'''Preparation of TOP10 chemically competent cells''' [http://openwetware.org/wiki/TOP10_chemically_competent_cells Igem Standard Protocol] <br>
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*'''Preparation of TOP10 chemically competent cells''' [http://openwetware.org/wiki/TOP10_chemically_competent_cells Igem Standard Protocol] <br>
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'''Transformation of competent cells (Mariels/Barbaras Protocol)''' [[Team:ESBS-Strasbourg/Transformation Barbara|Transformation]]<br>
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*'''Transformation of competent cells (Mariels/Barbaras Protocol)''' [[Team:ESBS-Strasbourg/Transformation Barbara|Transformation]]<br>
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Revision as of 22:31, 14 July 2008

E.Coli

  • Preparation of TOP10 chemically competent cells [http://openwetware.org/wiki/TOP10_chemically_competent_cells Igem Standard Protocol]
  • Transformation of competent cells (Mariels/Barbaras Protocol) Transformation


Cloning

Solutions and buffers

SOB for the preparation of competent cells [http://openwetware.org/wiki/SOB SOB]