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Wisconsin: Lignin Project/25 June 2008
From 2008.igem.org
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'''Team Fungus:''' <br> | '''Team Fungus:''' <br> | ||
| - | + | Digested PCR product and pET28a vector<br> | |
| - | Ran gel of digestion and performed gel purification<br> | + | Ran 1% agrose gel of digestion and performed gel purification<br> |
| + | -digested and undigested PCR product as well as digested vector showed correctly sized bands <br> | ||
| + | -undigested vector showed up at about 3kb when compared to supercoil ladder and at 4kb when compared to linear 1kb ladder <br> | ||
| + | Ran the rest of digestion reaction out on another 1% agrose gel and purified using QIAquick gel extraction kit | ||
---- | ---- | ||
'''Team Sorbitol:'''<br> | '''Team Sorbitol:'''<br> | ||
Revision as of 23:21, 14 July 2008
Team Fungus:
Digested PCR product and pET28a vector
Ran 1% agrose gel of digestion and performed gel purification
-digested and undigested PCR product as well as digested vector showed correctly sized bands
-undigested vector showed up at about 3kb when compared to supercoil ladder and at 4kb when compared to linear 1kb ladder
Ran the rest of digestion reaction out on another 1% agrose gel and purified using QIAquick gel extraction kit
Team Sorbitol:
