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Template:Team:UC Berkeley/Notebook/SC construction
From 2008.igem.org
(Difference between revisions)
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== pBca1256-K112403 == | == pBca1256-K112403 == | ||
<pre> | <pre> | ||
| - | Construction of { | + | Construction of {PhoA>} basic part K112403 |
CR cb10007/cb10008 on MG1655 (95 bp, EcoRI/BamHI) | CR cb10007/cb10008 on MG1655 (95 bp, EcoRI/BamHI) | ||
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== pBca1256-K112404 == | == pBca1256-K112404 == | ||
<pre> | <pre> | ||
| - | Construction of { | + | Construction of {LamB>} basic part K112404 |
PCR cb10009/cb10010 on MG1655 (107 bp, EcoRI/BamHI) | PCR cb10009/cb10010 on MG1655 (107 bp, EcoRI/BamHI) | ||
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== pBca1256-K112605 == | == pBca1256-K112605 == | ||
<pre> | <pre> | ||
| - | Construction of {a~ | + | Construction of {a~PhoA>} basic part K112605 |
PCR sc008/cb10008-phoA-R on MG1655 (100 bp, EcoRI/BamHI) | PCR sc008/cb10008-phoA-R on MG1655 (100 bp, EcoRI/BamHI) | ||
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== pBca1256-K112606 == | == pBca1256-K112606 == | ||
<pre> | <pre> | ||
| - | Construction of {a~ | + | Construction of {a~LamB>} basic part K112606 |
PCR sc009/cb10010-lamB-R on MG1655 (111 bp, EcoRI/BamHI) | PCR sc009/cb10010-lamB-R on MG1655 (111 bp, EcoRI/BamHI) | ||
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== pBca1256-K112607 == | == pBca1256-K112607 == | ||
<pre> | <pre> | ||
| - | Construction of { | + | Construction of {rbs_PhoA>} basic part K112607 |
PCR sc010/cb10008-PhoA-R on MG1655 (113 bp, EcoRI/BamHI) | PCR sc010/cb10008-PhoA-R on MG1655 (113 bp, EcoRI/BamHI) | ||
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== pBca1256-K112608 == | == pBca1256-K112608 == | ||
<pre> | <pre> | ||
| - | Construction of { | + | Construction of {rbs_LamB>} basic part K112608 |
PCR sc011/cb10010-lamB-R on E.coli K12 MG1655 (126 bp, EcoRI/BamHI) | PCR sc011/cb10010-lamB-R on E.coli K12 MG1655 (126 bp, EcoRI/BamHI) | ||
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== pBca1256-K112609 == | == pBca1256-K112609 == | ||
<pre> | <pre> | ||
| - | Construction of {a~ | + | Construction of {a~PhoA>} basic part K112609 (K112605 replacement) |
PCR sc012/cb10008-phoA-R on MG1655 (100 bp, EcoRI/BamHI) | PCR sc012/cb10008-phoA-R on MG1655 (100 bp, EcoRI/BamHI) | ||
Revision as of 01:51, 15 July 2008
Contents |
pBca1256-K112600
Construction of <AP-tag> basic part K112600 Wobble PCR SC001/SC002 (66 bp, EcoRI/BamHI) Sub into pBca1256 (EcoRI/BamHI, 2227+ 910) Product is pBca1256-K112600 ---------------------------------------------------- SC001F Forward Biobricking of <AP-tag> cgataGAATTCatgAGATCTggcctgaacgatatttttgaagcgcag SC002R Reverse Biobricking of <AP-tag> ccagtGGATCCttcatgccattcaattttctgcgcttcaaaaatatcg
pBca1256-K112601
Construction of <AP-tag! basic part K112601 Wobble PCR SC001/SC003 (69 bp, EcoRI/BamHI) Sub into pBca1256 (EcoRI/BamHI, 2227+ 910) Product is pBca1256-K112601 -------------------------------------------------- SC001 Forward Biobricking of <AP-tag! cgataGAATTCatgAGATCTggcctgaacgatatttttgaagcgcag SC003 Reverse Biobricking of <AP-tag! ccagtGGATCCttattcatgccattcaattttctgcgcttcaaaaatatcg
pBca1256-K112602
Construction of <FLAG-tag> basic part K112602 Wobble PCR SC004/SC005 (45 bp, EcoRI/BamHI) Sub into pBca1256 (EcoRI/BamHI, 2227+ 910) Product is pBca1256-K112602 --------------------------------------------------- SC004 Forward Biobricking of <FLAG-tag> cgataGAATTCatgAGATCTgactacaaggatgacgacg SC005 Reverse Biobricking of <FLAG-tag> ccagtGGATCCcttgtcgtcgtcatccttgtagtc
pBca1256-K112603
Construction of <FLAG-tag! basic part K112603 Wobble PCR SC004/SC006 (48 bp, EcoRI/BamHI) Sub into pBca1256 (EcoRI/BamHI, 2227+ 910) Product is pBca1256-K112603 ----------------------------------------------------- SC004 Forward Biobricking of <FLAG-tag! cgataGAATTCatgAGATCTgactacaaggatgacgacg SC006 Reverse Biobricking of <FLAG-tag! ccagtGGATCCttacttgtcgtcgtcatccttgtagtc
pBca1256-K112604
Construction of {rbs_barstar>} basic part K112604
PCR mea035/sc007 on Bacillus amyloliquefaciens gen. (320 bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112604
-------------------------------
mea035 Fwd biobricking of {rbs_barstar>}
cgataGAATTCatgAGATCTcataagaaaggagccgcacatg
sc007 Reverse biobricking of {rbs_barstar>} (Not needed...)
cgttaGGATCCagaaagtatgatggtgatgtcgc
pBca1256-K112403
Construction of {PhoA>} basic part K112403
CR cb10007/cb10008 on MG1655 (95 bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112403
-------------------------------------------------------------
cb10007 Fwd biobricking of {prepro>}
cgataGAATTCatgAGATCTatgaaacaaagcactattgcactggc
cb10008 Reverse biobricking of {prepro>}
cgtagGGATCCgggcttttgtcacaggggtaaac
pBca1256-K112404
Construction of {LamB>} basic part K112404
PCR cb10009/cb10010 on MG1655 (107 bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112404
-------------------------------------------------------------
cb10009 Fwd biobricking of {prepro>}
cgataGAATTCatgAGATCTatgatgattactctgcgcaaac
cb10010 Reverse biobricking of {prepro>}
cgtagGGATCCcagccattgcctgagcagacattac
pBca1256-K112605
Construction of {a~PhoA>} basic part K112605
PCR sc008/cb10008-phoA-R on MG1655 (100 bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112605
------------------------------------------------------
sc008 Fwd biobricking of {a~prepro>}
cgataGAATTCatgAGATCTtaaaGtgaaacaaagcactattgcactggc
cb10008 Reverse biobricking of {a~prepro>}
cgtagGGATCCgggcttttgtcacaggggtaaac
pBca1256-K112606
Construction of {a~LamB>} basic part K112606
PCR sc009/cb10010-lamB-R on MG1655 (111 bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256- K112606
--------------------------------------------------
sc009 Fwd biobricking of {a~prepro>}
cgataGAATTCatgAGATCTtagaatgatgattactctgcgc
cb10010 Reverse biobricking of {a~prepro>}
cgtagGGATCCcagccattgcctgagcagacattac
pBca1256-K112607
Construction of {rbs_PhoA>} basic part K112607
PCR sc010/cb10008-PhoA-R on MG1655 (113 bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112607
------------------------------------------------------
sc010 Fwd biobricking of {rbs_prepro>}
cgataGAATTCatgAGATCTgtacatggagaaaataaaAtgaaacaaagcac
cb10008 Reverse biobricking of {rbs_prepro>}
cgtagGGATCCgggcttttgtcacaggggtaaac
pBca1256-K112608
Construction of {rbs_LamB>} basic part K112608
PCR sc011/cb10010-lamB-R on E.coli K12 MG1655 (126 bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112608
------------------------------------------------------
sc011 Fwd biobricking of {rbs_prepro>}
cgataGAATTCatgAGATCTcaatgactcaggagatagaatg
cb10010 Reverse biobricking of {rbs_prepro>}
cgtagGGATCCcagccattgcctgagcagacattac
pBca1256-K112609
Construction of {a~PhoA>} basic part K112609 (K112605 replacement)
PCR sc012/cb10008-phoA-R on MG1655 (100 bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112605
------------------------------------------------------
sc012 Fwd biobricking of {a~prepro>}
cgataGAATTCatgAGATCTtaaaAtgaaacaaagcactattgcactggc
cb10008 Reverse biobricking of {a~prepro>}
cgtagGGATCCgggcttttgtcacaggggtaaac
