Latest revision as of 03:41, 15 July 2008

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Things we did today

Krystle

Followed protocol used for transformation of cells with BioBrick plasmids from filter

pilA, slr1, slr2 combined with homemade competent cells (batch 3)

pnir combined with commercial supercompetent cells.

Krystle

colony purification for downstream culture-upscaling and preparation of DB3.1 competent cells
DB3.1 strain will be used to accept plasmids containing the ccdB "death gene"
Sm plate grew, plate lacking antibiotics grew, Kan plate did not grow (as expected)

[[Image:

Margaret

Grace

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson

[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]

@@ Line 9: / Line 9: @@
 :::: pilA, slr1, slr2 combined with homemade competent cells (batch 3)
 :::: pnir combined with commercial supercompetent cells.
+===Streak Out DB3.1===
+:'''Krystle'''
+:* colony purification for downstream culture-upscaling and preparation of DB3.1 competent cells
+:* DB3.1 strain will be used to accept plasmids containing the ccdB "death gene"
+:* Sm plate grew, plate lacking antibiotics grew, Kan plate did not grow (as expected)
+[[Image:
+===Run PCR Products on Gel===
+:'''Margaret'''
+:* PCR amplification of Omega cassette and OriR
+:* see [[Team:Hawaii/PCR_Amplification_of_pRL1383a| here for updates for the pRL1383a amplification]]
 == Drylab Work ==