"
Team:NTU-Singapore/Notebook/14 July 2008
From 2008.igem.org
(Difference between revisions)
m |
|||
| Line 1: | Line 1: | ||
*LacI-GFP COLONIES!! | *LacI-GFP COLONIES!! | ||
**Miniprep of 18 colonies picked and one for T7ptag | **Miniprep of 18 colonies picked and one for T7ptag | ||
| - | **Gel check was ran - 5 colonies had properly ligated LacI-GFP, but T7ptag was not ligated well. | + | **Gel check was ran - 5 colonies had properly ligated LacI-GFP (got 1.1kb band), but T7ptag was not ligated well. |
Well Done! | Well Done! | ||
| + | *Ligation of Fe and GFP was also carried out. | ||
| + | **pFe insert from last Friday (stored at -20). We also did nanodrops before ligating to measure the concentration. | ||
| + | **GFP vector also from last Friday digestion. | ||
| + | **This time, we used 2 insert:vector ratios (1:3 and 1:6). | ||
Revision as of 11:18, 15 July 2008
- LacI-GFP COLONIES!!
- Miniprep of 18 colonies picked and one for T7ptag
- Gel check was ran - 5 colonies had properly ligated LacI-GFP (got 1.1kb band), but T7ptag was not ligated well.
Well Done!
- Ligation of Fe and GFP was also carried out.
- pFe insert from last Friday (stored at -20). We also did nanodrops before ligating to measure the concentration.
- GFP vector also from last Friday digestion.
- This time, we used 2 insert:vector ratios (1:3 and 1:6).
