"
User:University of Washington/15 July 2008
From 2008.igem.org
(Difference between revisions)
(→RP4 Conjugation) |
|||
| Line 7: | Line 7: | ||
·DH5alpha + RP4<br> | ·DH5alpha + RP4<br> | ||
·DH5alpha + pCS26-pac | ·DH5alpha + pCS26-pac | ||
| + | |||
| + | |||
| + | == LuxR from AraC and TetR == | ||
| + | |||
| + | - Miniprepped 12 overnight culture of mutated AraC into 30 ul EB | ||
| + | |||
| + | - Ordered sequences of all 12: 8 ul (1 umol/mL) forward primer + 4 ul DNA | ||
| + | |||
| + | - Grew 4 overnight culture of D29 from Elowitz's lab | ||
Revision as of 21:34, 15 July 2008
RP4 Conjugation
Mini prep
·pAC88
Bac-Bac conjugation protocol #1
·DH5alpha + RP4
·DH5alpha + pCS26-pac
LuxR from AraC and TetR
- Miniprepped 12 overnight culture of mutated AraC into 30 ul EB
- Ordered sequences of all 12: 8 ul (1 umol/mL) forward primer + 4 ul DNA
- Grew 4 overnight culture of D29 from Elowitz's lab
Back to Team:University_of_Washington/Notebook#Notebook
