Paris/July 11

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[[Paris/July 10|yesterday]] -- [[Paris/July 12|tomorrow]] <br>
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== Cultures ==
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The strains stocked at -80°C were taken out and spread (streaks) on LB boxes (without antibiotic).
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Boxes have been put in the steam room at 37°C at 15h45
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{| border="1"
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|align="center"|'''Code'''
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|align="center"|'''Strain'''
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|DW 27
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|MG1655 ΔfliA::Cm
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|NEC 283
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|MG 1655 ΔfliA::FRT
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|-
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|NEC 284
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|MG 1655 ΔflhD::FRT
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|-
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|DW 26
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|MG 1655 ΔflgM::Cm
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|NEC 285
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|MG 1655 ΔflgM::FRT
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|-
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|DW 35
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|JW K1879 ΔmotA::Kan
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|NEC 288
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|MG 1655 ΔmotA::FRT
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|DW 37
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|JW K1068 ΔflgJ::Kan
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|NEC 287
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|MG 1655 ΔflgJ::FRT
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|DW 24
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|MG 1655 ΔfliC::Cm
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|NEC 286
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|MG 1655 ΔfliC::FRT
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|}
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== Manipulations ==
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* Culture of DH5ɑ in 5mL of LB
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* Preparation of 250mL of CaCl_2 0,1M
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* Transfer of the equipment iGEM 07 on the sixth floor
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Latest revision as of 08:44, 16 July 2008

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