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Team:ESBS-Strasbourg/14 July 2008
From 2008.igem.org
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=== Wiki === | === Wiki === | ||
*Navigation and Header (MM) | *Navigation and Header (MM) | ||
| + | *Getting stucked in IRESes: the ccdIRES works only in humans... | ||
=== Modeling === | === Modeling === | ||
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*Media | *Media | ||
| - | ** bacteria: LB-medium (500 ml), LB-medium | + | ** bacteria: LB-medium (500 ml), LB-medium with Ampicillin (250 ml), LB-Agar-plates without antibiotics (7) |
** yeast: YPD-medium (500 ml) | ** yeast: YPD-medium (500 ml) | ||
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Latest revision as of 16:52, 16 July 2008
Contents |
DryLab
Wiki
- Navigation and Header (MM)
- Getting stucked in IRESes: the ccdIRES works only in humans...
Modeling
WetLab
results from the transformation
- 99 colonies of the control plasmid in TOP10(invitrogen), 5*10^6 cfu/ug (still not enough)
- 1 colonie of the BBa_J63002(Adh1_ter) in TOP10(invitrogen), PCR with primers <<verification pSB1A3 left/right>> should deliver a 461 bp fragment
- Media
- bacteria: LB-medium (500 ml), LB-medium with Ampicillin (250 ml), LB-Agar-plates without antibiotics (7)
- yeast: YPD-medium (500 ml)
- Overnight cultures
- bacteria:
- yeast: strain BYA4742 (28°C)
