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Team:ESBS-Strasbourg/11 July 2008
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(New page: < back == DryLab == === Modeling === == WetLab == == General == == Quote of the day == "At least we have worked steril." (Marius)) |
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[[Team:ESBS-Strasbourg/Notebook | < back]] | [[Team:ESBS-Strasbourg/Notebook | < back]] | ||
== DryLab == | == DryLab == | ||
| + | |||
| + | michi: | ||
| + | *Planing of the measurements we will have to perform | ||
| + | *Get started with IRES: WOW their exist cell cycle dependent IRESes | ||
=== Modeling === | === Modeling === | ||
== WetLab == | == WetLab == | ||
| + | *results SD, MM and KK: | ||
| + | ** TOP10: 5* 10^5 cfu/ug | ||
| + | ** db3.1: 10^5 cfu/ug | ||
| + | ** dh5alpha: 10^6 cfu/ug | ||
| + | <br> | ||
| + | * Primer | ||
| + | ** the arrived verification primer were resuspended to give a stock solution of 50 µM | ||
| + | ** aliquots of these primers (4x 10 µM) were produced and everything frozen (-20°C) | ||
| + | <br> | ||
| + | * Transformation | ||
| + | ** competent Top10 cells from Invitrogen, protocol from Marias Lab: H2O (negative control), pxj (positive control), 5 BioBricks (EYFP, vector without insert, adh terminator, lacI, lamdba CI), plated on ampicillin | ||
| + | ** competent db3.1 cells, protocol from Barbaras Lab, DNA was already in KCM and cells were just added and plated, K3 on kanamycin and A2 on ampicillin | ||
| + | ** competent dh5alpha cells, protocol from iGEM using SOC medium: H2O (negative control), pxj (positive control), BioBrick (EYFP), plated on ampicillin | ||
== General == | == General == | ||
Latest revision as of 16:53, 16 July 2008
Contents |
DryLab
michi:
- Planing of the measurements we will have to perform
- Get started with IRES: WOW their exist cell cycle dependent IRESes
Modeling
WetLab
- results SD, MM and KK:
- TOP10: 5* 10^5 cfu/ug
- db3.1: 10^5 cfu/ug
- dh5alpha: 10^6 cfu/ug
- Primer
- the arrived verification primer were resuspended to give a stock solution of 50 µM
- aliquots of these primers (4x 10 µM) were produced and everything frozen (-20°C)
- Transformation
- competent Top10 cells from Invitrogen, protocol from Marias Lab: H2O (negative control), pxj (positive control), 5 BioBricks (EYFP, vector without insert, adh terminator, lacI, lamdba CI), plated on ampicillin
- competent db3.1 cells, protocol from Barbaras Lab, DNA was already in KCM and cells were just added and plated, K3 on kanamycin and A2 on ampicillin
- competent dh5alpha cells, protocol from iGEM using SOC medium: H2O (negative control), pxj (positive control), BioBrick (EYFP), plated on ampicillin
General
Quote of the day
"At least we have worked steril." (Marius)
