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Team:University of Ottawa/15 July 2008
From 2008.igem.org
(Difference between revisions)
(New page: Transformation of XL10 Competent E. coli cells with pDR197:AtCKX2 1 uL Pure pDR197:AtCKX2 1 ul 1:10 dilution pDR197:AtCKX2 1 ul 1:100 dilution pDR197:AtCKX2 100 ul XL10 E.Coli per reaction...) |
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| - | Transformation of XL10 Competent E. coli cells with pDR197:AtCKX2 | + | __TOC__ |
| - | 1 uL Pure pDR197:AtCKX2 | + | ==Today on the Lab=== |
| - | 1 ul 1:10 dilution pDR197:AtCKX2 | + | '''Tammy''' |
| - | 1 ul 1:100 dilution pDR197:AtCKX2 | + | :<li>Transformation of XL10 Competent E. coli cells with pDR197:AtCKX2 |
| - | 100 ul XL10 E.Coli per reaction | + | :<li>1 uL Pure pDR197:AtCKX2 |
| - | 900 ul LB Broth per reaction | + | :<li>1 ul 1:10 dilution pDR197:AtCKX2 |
| - | 3 LB Ampicillin (50 ug/mL) Plates | + | :<li>1 ul 1:100 dilution pDR197:AtCKX2 |
| - | Began Incubation of transformed cells at 1:50 pm. | + | :<li>100 ul XL10 E.Coli per reaction |
| + | :<li>900 ul LB Broth per reaction | ||
| + | :<li>3 LB Ampicillin (50 ug/mL) Plates | ||
| + | :<li>Began Incubation of transformed cells at 1:50 pm. | ||
Revision as of 19:17, 16 July 2008
Contents |
Today on the Lab=
Tammy
- Transformation of XL10 Competent E. coli cells with pDR197:AtCKX2
- 1 uL Pure pDR197:AtCKX2
- 1 ul 1:10 dilution pDR197:AtCKX2
- 1 ul 1:100 dilution pDR197:AtCKX2
- 100 ul XL10 E.Coli per reaction
- 900 ul LB Broth per reaction
- 3 LB Ampicillin (50 ug/mL) Plates
- Began Incubation of transformed cells at 1:50 pm.
