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Team:ESBS-Strasbourg/TENS
From 2008.igem.org
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*0.4 mL of 10 M NaOH | *0.4 mL of 10 M NaOH | ||
*1 mL of 20% SDS | *1 mL of 20% SDS | ||
| - | *fill up to 40 mL with milliQ | + | *fill up to 40 mL with milliQ water |
Revision as of 09:50, 17 July 2008
TENS
Tris-HCl 10 mM pH8
EDTA 1 mM pH8
NaOH 0.1 M
SDS 0.5%
Make a Sterilfiltration of the solution or use steril stock solutions for its preparation
For 40 mL of TENS:
- 0.8 mL of 0.5M Tris-HCL pH8
- 0.1 mL of 0.4M EDTA
- 0.4 mL of 10 M NaOH
- 1 mL of 20% SDS
- fill up to 40 mL with milliQ water
