"
Team:ESBS-Strasbourg/TENS
From 2008.igem.org
(Difference between revisions)
| Line 6: | Line 6: | ||
Make a Sterilfiltration of the solution or use steril stock solutions for its preparation | Make a Sterilfiltration of the solution or use steril stock solutions for its preparation | ||
<br> | <br> | ||
| - | + | <br> | |
'''For 40 mL of TENS:''' | '''For 40 mL of TENS:''' | ||
*0.8 mL of 0.5M Tris-HCL pH8 | *0.8 mL of 0.5M Tris-HCL pH8 | ||
Latest revision as of 09:56, 17 July 2008
Tris-HCl 10 mM pH8
EDTA 1 mM pH8
NaOH 0.1 M
SDS 0.5%
Make a Sterilfiltration of the solution or use steril stock solutions for its preparation
For 40 mL of TENS:
- 0.8 mL of 0.5M Tris-HCL pH8
- 0.1 mL of 0.4M EDTA
- 0.4 mL of 10 M NaOH
- 1 mL of 20% SDS
- fill up to 40 mL with milliQ water
