Minnesota/18 July 2008

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Revision as of 21:22, 18 July 2008

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1. Analyze gel results from 07-17-2008: Send in select samples from the gel for sequencing. Send in reaction mixture containing template DNA and primers that will bind to it, and thus allow to sequence. Sequencing L3i, L3j, L4b, L4i, L5c, L6a, L6b, L6d, L6e, L7a, L7b, L7d, and L8a.

2. Problem: No growth on plates with MCherry, RFP, Terminator, and TetR promoter.

Solution: Re-transform (again) paper DNA with MCherry, RFP, Term., and TetR.

3. Double Digest and Ligate: Double digesting then ligating dually repressed promoters; (1) Lac/LAMBDAcI, (2) TetR/p22mnt, along with reporter genes; GFP and YFP.

RFP will not be used because it has not been properly transformed yet. Will result in:

Lac/LAMBDAcI:GFP:Terminator

Tet/p22mnt:YFP:Terminator

4. Work on model: Model allows us to run potential reactions computationally. This will give us an idea of whether or not there will be errors in the rxns and what outputs will result.

@@ Line 13: / Line 13: @@
 ''Solution:'' Re-transform (again) paper DNA with MCherry, RFP, Term., and TetR.
 |-
-|'''3. Double Digest and Ligate:''' Double digesting then ligating dually repressed promoters; (1) Lac/LAMBDAcI, (2) TetR/p22mnt, along with reporter genes; GFP and YFP. RFP will not be used because it has not been properly transformed yet. Will result in:
+|'''3. Double Digest and Ligate:''' Double digesting then ligating dually repressed promoters; (1) Lac/LAMBDAcI, (2) TetR/p22mnt, along with reporter genes; GFP and YFP.
+RFP will not be used because it has not been properly transformed yet. Will result in:
 |-
 |Lac/LAMBDAcI:GFP:Terminator