Team:The University of Alberta/22 July 2008

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(New page: Mike added links to two protein complementation papers in the Project/Documents section of the Wiki. One of these uses a Vibrio transcription factor (ToxR) fragment and Vibrio promoter in...)
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Mike added links to two protein complementation papers in the Project/Documents section of the Wiki.  One of these uses a Vibrio transcription factor (ToxR) fragment and Vibrio promoter in E. coli, and the other uses B-galactosidase in E. coli.  Can anyone figure out how to make the appropriate BioBricks to fuse these to the existing ER LBD BioBrick?  We should be able to amplify the B-galactosidase fragments from E. coli, but we will need to synthesize the Vibrio fragments and promoter.  We will also need to order a lacZ deletion strain of E. coli (listed in the paper) if we don't already have something like it.
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Mike added links to two protein complementation papers in the Project/Journals section of the Wiki.  One of these uses a Vibrio transcription factor (ToxR) fragment and Vibrio promoter in E. coli, and the other uses B-galactosidase in E. coli.  Can anyone figure out how to make the appropriate BioBricks to fuse these to the existing ER LBD BioBrick?  We should be able to amplify the B-galactosidase fragments from E. coli, but we will need to synthesize the Vibrio fragments and promoter.  We will also need to order a lacZ deletion strain of E. coli (listed in the paper) if we don't already have something like it.
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Can anyone commit to designing these primers and synthetic constructs (ASAP?)

Revision as of 21:43, 18 July 2008

Mike added links to two protein complementation papers in the Project/Journals section of the Wiki. One of these uses a Vibrio transcription factor (ToxR) fragment and Vibrio promoter in E. coli, and the other uses B-galactosidase in E. coli. Can anyone figure out how to make the appropriate BioBricks to fuse these to the existing ER LBD BioBrick? We should be able to amplify the B-galactosidase fragments from E. coli, but we will need to synthesize the Vibrio fragments and promoter. We will also need to order a lacZ deletion strain of E. coli (listed in the paper) if we don't already have something like it.

Can anyone commit to designing these primers and synthetic constructs (ASAP?)

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