Team:Paris/Notebook/Freezer

From 2008.igem.org

(Difference between revisions)
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== PCR Products==
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|}
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-
== Plasmids : MiniPrep Products==
 
 +
== Plasmids : MiniPrep Products==
{|
{|
-
|MP 1
+
|- style="background: #ccccff;"
-
|-
+
! colspan="5" style="background: #ccccff;" | Plasmids: MiniPrep Products
-
|MP 2
+
|- style="background: #ccccff; text-align: center;"
-
|-
+
|width=5%| Number
-
|MP 3
+
|width=25%| Name
-
|-
+
|width=50%| Description
-
|MP 4
+
|width=15%| Products in use
-
|-
+
|width=5%| Date
-
|MP 5
+
|- style="background: #cccccc;" 
-
|-
+
| style="background: #ccffcc;" |MP1
-
|MP 6
+
|<bbpart>pJ23100</bbpart> in <bbpart>J61002</bbpart>
-
|-
+
|Strong constitutive promoter in J61002 (clones 1 + 2) <partinfo>pJ23100 SpecifiedComponents</partinfo>
-
|MP 7
+
|
-
|-
+
| [[Paris/July 15| July 15]], [[Paris/August 17| Aug. 17]]
-
|MP 8
+
|- style="background: #cccccc;" 
-
|-
+
| style="background: #ccffcc;" |MP2
-
|MP 9
+
|<bbpart>pJ23107</bbpart> in <bbpart>J61002</bbpart>
 +
|Medium constitutive promoter in J61002 (clones 1 + 2) <partinfo>pJ23107 SpecifiedComponents</partinfo>
 +
|MP2.2
 +
| [[Paris/July 15| July 15]], [[Paris/August 17| Aug. 17]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |MP3
 +
|<bbpart>B0015</bbpart> in <bbpart>pSB1AK3</bbpart>
 +
|Double terminator (clones 1 + 2) <partinfo>B0015 SpecifiedComponents</partinfo>
 +
|MP3.3, MP3.4
 +
| [[Paris/August 14| August 14]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |MP4
 +
|<bbpart>I0500</bbpart> in <bbpart>pSB2K3</bbpart>
 +
|AraC pBAD (clones 1 + 2) <partinfo>I0500 SpecifiedComponents</partinfo>
 +
|
 +
| [[Paris/July 16| July 16]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |MP5
 +
|<bbpart>BBa_B0030</bbpart> in <bbpart>pSB1A2</bbpart>
 +
|Strong RBS (clones 1 + 2) <partinfo>B0030 SpecifiedComponents</partinfo>
 +
|
 +
| [[Paris/July 18| July 18]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |MP6
 +
|<bbpart>BBa_E0422</bbpart> in <bbpart>pSB1A2</bbpart>
 +
|ECFP (RBS+LVA+Term) (clones 1 + 2) <partinfo>E0422 SpecifiedComponents</partinfo>
 +
|MP6'.1, MP6'.2
 +
| [[Paris/July 18| July 18]], [[Paris/July 24| July 24]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |MP7
 +
|<bbpart>BBa_E0241</bbpart> in <bbpart>pSB1A2</bbpart>
 +
|PoPs to GFP converter (clones 1 + 2) <partinfo>BBa_E0241 DeepComponents</partinfo>
 +
|MP7'.1
 +
| [[Paris/July 18| July 18]], [[Paris/July 24| July 24]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |MP8
 +
|<bbpart>BBa_E0840</bbpart> in <bbpart>pSB1A2</bbpart>
 +
|gfp tri-part; strong rbs (clones 1 + 2) <partinfo>E0840 SpecifiedComponents</partinfo>
 +
|
 +
| [[Paris/July 18| July 18]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |MP9
 +
|<bbpart>BBa_J61047</bbpart> in <bbpart>pSB1A2</bbpart>
 +
|Cre ORF (clones 1 + 2) <partinfo>J61047 SpecifiedComponents</partinfo>
 +
|MP9'.1, MP9'.2
 +
| [[Paris/July 18| July 18]], [[Paris/July 24| July 24]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |MP10
 +
|<bbpart>BBa_I0500</bbpart> in <bbpart>pSB2K3</bbpart>
 +
|AraC pBAD (clones 1 + 2) <partinfo>I0500 SpecifiedComponents</partinfo>
 +
|
 +
| [[Paris/July 20| July 20]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |MP11
 +
|<bbpart>BBa_pJ23107</bbpart> in <bbpart>p61002</bbpart>
 +
|Medium constitutive promoter in J61002 (clones 3 + 4) <partinfo>pJ23107 SpecifiedComponents</partinfo>
 +
|MP11'.3
 +
| [[Paris/July 20| July 20]], [[Paris/July 23| July 23]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |MP12
 +
|<bbpart>BBa_P1003</bbpart> in <bbpart>pSB1A2</bbpart>
 +
|KanR <partinfo>P1003 SpecifiedComponents</partinfo>
 +
|
 +
| [[Paris/July 27| July 27]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |MP13
 +
|<bbpart>BBa_J61002</bbpart>
 +
|ptet-mRFP <partinfo>J61002 SpecifiedComponents</partinfo>
 +
|MP13.1, MP13.2
 +
| [[Paris/August 14| August 14]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |MP14
 +
|<bbpart>BBa_J61025</bbpart>
 +
|Medium constitutive promoter in J61002 (clones 1 + 2) <partinfo>BBa_J61025 SpecifiedComponents</partinfo>
 +
|MP14.1, MP14.2
 +
| [[Paris/August 14| August 14]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |L1
 +
|<bbpart>BBa_I0500</bbpart> in <bbpart>J61002</bbpart>
 +
|construct with pBad promotor regulating expression of mRFP <partinfo>I0500 SpecifiedComponents</partinfo>
 +
|L1".1, L1".2
 +
| [[Paris/August 14| August 14]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |
 +
|AttB
 +
|
 +
|MP15
 +
| [[Paris/August 14| August 14]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |
 +
|AttP
 +
|Finished
 +
|MP16
 +
| [[Paris/July 25| July 25]], [[Paris/July 27| July 27]], [[Paris/August 7| Aug 7]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |L2
 +
|Lox66DapAE.coli in <bbpart>pSB1A2</bbpart>
 +
|Insertion of lox66DapAE.coli construct as a biobrick in pSB1A2 plasmid
 +
http://parts.mit.edu/registry/images/9/9f/Part_icon_dna.png
 +
http://parts.mit.edu/registry/images/7/7b/Part_icon_cds.png
 +
|
 +
| [[Paris/July 25| July 25]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |L6
 +
|<bbpart>BBa_B0030</bbpart>+<bbpart>BBa_J61047</bbpart>
 +
|strong RBS(B0030)-Cre ORF
 +
http://parts.mit.edu/r/parts/images/partbypart/icon_rbs.png
 +
http://parts.mit.edu/registry/images/7/7b/Part_icon_cds.png
 +
|L6.1, L6.2, L6.3, L6".1, L6".2
 +
| [[Paris/July 25| July 25]], [[Paris/July 27| July 27]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |L9''
 +
|lox66 in <bbpart>pSB1A2</bbpart>
 +
|
 +
|
 +
|[[Paris/July 25| July 25]], [[Paris/July 30| July 30]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |L10''
 +
|lox71 in <bbpart>pSB1A2</bbpart>
 +
|
 +
|
 +
|[[Paris/July 25| July 25]], [[Paris/July 30| July 30]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |L12
 +
|lox71-gfp in pSB1A2
 +
|construction of the biobrick lox71-gfp
 +
http://parts.mit.edu/registry/images/9/9f/Part_icon_dna.png
 +
http://parts.mit.edu/r/parts/images/partbypart/icon_reporter.png
 +
|L12.1 & L12.2
 +
|[[Paris/July 30| July 30]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |L26
 +
|araC/pbad>>RBS-Cre in <bbpart>J61002</bbpart>
 +
|ligation ([[Paris/August 13| Aug.13]]) of RBS-Cre as a CDS behind pbad in <bbpart>J61002</bbpart>
 +
|L26.1 (no more) & L26.2
 +
|[[Paris/August 17| Aug. 17]]
 +
|- style="background: #cccccc;"
 +
| style="background: #ccffcc;" |L29
 +
|gfptripart-lox66 <bbpart>pSB1A2</bbpart>
 +
|ligation ([[Paris/August 13| Aug.13]]) of gfp-tripart in front of lox66
 +
|L29.1 & L29.2
 +
|[[Paris/August 17| Aug. 17]]
 +
|- style="background: #cccccc;"
 +
| style="background: #ccffcc;" |L30
 +
|dapAp>>mRFP in <bbpart>J61002</bbpart>
 +
|dapAp promotor placed([[Paris/August 13| Aug.13]]) upstream of mRFP <bbpart>J61002</bbpart>
 +
|L30A1 & L30A2
 +
|[[Paris/August 17| Aug. 17]]
 +
|- style="background: #cccccc;"
 +
| style="background: #ccffcc;" |L31
 +
|lox71-<bbpart>B0015</bbpart>(T) in <bbpart>pSB1A2</bbpart>
 +
|
 +
|L31.1 & L31.3
 +
|[[Paris/August 20| Aug. 20]]
 +
|- style="background: #cccccc;"
 +
| style="background: #ccffcc;" |L32
 +
|<bbpart>B0015</bbpart>(T)-lox66 in <bbpart>pSB1A2</bbpart>
 +
|
 +
|L32.1 & L32.2
 +
|[[Paris/August 20| Aug. 20]]
 +
|- style="background: #cccccc;"
 +
| style="background: #ccffcc;" |L34
 +
|pTet>>lox71-gfptripart in <bbpart>pSB1A2</bbpart>
 +
|
 +
|L34.1 & L34.2
 +
|[[Paris/August 20| Aug. 20]]
 +
|- style="background: #cccccc;"
 +
| style="background: #ccffcc;" |L35
 +
|araC/pBad>>lox71-gfptripart in <bbpart>pSB1A2</bbpart>
 +
|il manque la seq. de 740-1410
 +
|L35.1 & L35.2
 +
|[[Paris/August 20| Aug. 20]]
 +
|- style="background: #cccccc;"
 +
| style="background: #ccffcc;" |L36
 +
|lox66-mRFP
 +
|
 +
|L36.1 & L36.2
 +
|[[Paris/August 20| Aug. 20]]
 +
|- style="background: #cccccc;"
 +
| style="background: #ccffcc;" |L37
 +
|lox66-RBS-dapAcoli
 +
|
 +
|L37.1 & L37.2
 +
|[[Paris/August 20| Aug. 20]]
 +
|- style="background: #cccccc;"
 +
| style="background: #ccffcc;" |L39
 +
|RBS-dapAcoli cloned in <bbpart>pSB1A2</bbpart>
 +
|
 +
|L39.1 & L39.2
 +
|[[Paris/August 20| Aug. 20]]
 +
|- style="background: #cccccc;"
 +
| style="background: #ccffcc;" |L46
 +
|pTet>>lox71-B0015(T) in <bbpart>pSB1A2</bbpart>
 +
|
 +
|L46.1
 +
|[[Paris/September 18| Sep. 18]]
 +
|- style="background: #cccccc;"
 +
| style="background: #ccffcc;" |L53
 +
|B0015(T)-lox66-RBSDapAcoli in <bbpart>pSB1A2</bbpart>
 +
|
 +
|L53.7
 +
|[[Paris/September 18| Sep. 18]]
 +
|- style="background: #cccccc;"
 +
| style="background: #ccffcc;" |L56
 +
|B0015(T)-lox66-RBSdapAsubtilis in <bbpart>pSB1A2</bbpart>
 +
|
 +
|L56.3 (attention, seq a partiellement foiré, refaire seq)
 +
|[[Paris/September 18| Sep. 18]]
 +
|- style="background: #cccccc;"
 +
| style="background: #ccffcc;" |L59
 +
|pTet>>lox71-gfptripart-lox66-mRFP <bbpart>pSB1A2</bbpart>
 +
|
 +
|L59.6 (attention, seq avec O40 a foiré, mais les extrémités sont bonnes, je pense que c ok car les colonies sont vertes)
 +
|[[Paris/September 18| Sep. 18]]
 +
|- style="background: #cccccc;"
 +
| style="background: #ccffcc;" |L64
 +
|RBSdapAsubtilis cloned in <bbpart>pSB1A2</bbpart>
 +
|
 +
|L564.1 & L64.2
 +
|[[Paris/September 18| Sep. 18]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |MPT1
 +
|Lox66-DapAColi
 +
|pTOPO P5
 +
|MPT1.1, MPT1.2
 +
|[[Paris/August 7| Aug 7]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |MPT3
 +
|Lox66-DapASubtilis
 +
|pTOPO P6
 +
|MPT3.2, MPT3.1
 +
|[[Paris/August 7| Aug 7]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |MPT4
 +
|RBS-DapAColi
 +
|pTOPO P8
 +
|MPT4.1, .2, .3, .4& .5
 +
|[[Paris/August 7| Aug 7]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |MPT5
 +
|RBS-DapASubtilis
 +
|pTOPO P9
 +
|MPT5.1, .2 &.3
 +
|[[Paris/August 7| Aug 7]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |MP15
 +
|pKD46
 +
|
 +
|
 +
|[[Paris/October 2| Oct 2]]
 +
|}

Revision as of 11:13, 21 July 2008

PCR Products

PCR products
Number Name Oligo F Oligo R Matrix Length Comments Product Left ? []ng/µL Date
P1 Lox71-FtsA-FtsZ-1 O3 Lox71-FtsA-F O4 DEcoR1-FtsZ-R MG1655 1537 Ø
P2 FtsZ-2 O5 DEcoR1-FtsZ-F O2 FtsZ-R MG1655 1088 Ø
P3 DGAT-1 27 ForDGAT1 13 DPst1-DGAT-R Miniprep pKS::DGAT 1087 July 31
P4 DGAT-2 12 DPst1-DGAT 27 Rev-DGAT1 Miniprep pKS::DGAT 396 July 31
P5 Lox66-DapAColi O6 Lox66-DapAColi-F O7 DapAColi-R MG1655 985 Ø July 17, July 30
P6 Lox66-DapASubtilis O8 Lox66-DapASubt-F O9 DapASubt-R B.Subtilis 168 979 July 17, July 27, July 30
P7 Lox71-FtsZ O1 Lox71-FtsZ-F O2 FtsZ-R MG1655 1260 EcoRI site in FtsZ not mutated July 17, July 30
P8 RBS-DapAColi O20 RBS-DapAColi-F O7 DapAColi-R MG1655 952 B0030-DapAColi July 23, July 30
P9 RBS-DapASubtilis O21 RBS-DapASubt-F O9 DapASubt-R B.Subtilis 168 946 B0030-DapASubtilis July 23, July 27
P10 Lox71-FtsA-FtsZ O3 Lox71-FtsA-F O2 FtsZ-R MG1655 2580 EcoRI site in FtsZ not mutated July 23
P11 Lox71-KmR-Lox66 O10 Lox71-KmR-F O11 Lox66-KmR-R MP12 1084 July 27, July 30
P13 DGAT deltaPst-1 ForDGAT1 Megaprimer DGAT2 DNA plasmid (pKS::DGAT): 1443 August 1
P14 DGAT deltaPst-1 biobrick ForDGAT1bb RevDGAT1bb DGAT deltaPst1 1504 August 2 Oct. 18
P15 PacI [pTet>>lox-GFP-loxmRFP] XmaI O47 O48 [pTet>>lox-GFP-loxmRFP] L59.6 2kb August 2
P16 PrefixBB-frt1-CmR-frt2-SufixBB O51 O52 pY-2C-IntC 1kb Oct. 15
P17 PrefixBB-frt1-CmR-frt2-T1T2-SufixBB O51 O53 pY-2C-IntC >1kb Oct. 18


Plasmids : MiniPrep Products

Plasmids: MiniPrep Products
Number Name Description Products in use Date
MP1 <bbpart>pJ23100</bbpart> in <bbpart>J61002</bbpart> Strong constitutive promoter in J61002 (clones 1 + 2) <partinfo>pJ23100 SpecifiedComponents</partinfo> July 15, Aug. 17
MP2 <bbpart>pJ23107</bbpart> in <bbpart>J61002</bbpart> Medium constitutive promoter in J61002 (clones 1 + 2) <partinfo>pJ23107 SpecifiedComponents</partinfo> MP2.2 July 15, Aug. 17
MP3 <bbpart>B0015</bbpart> in <bbpart>pSB1AK3</bbpart> Double terminator (clones 1 + 2) <partinfo>B0015 SpecifiedComponents</partinfo> MP3.3, MP3.4 August 14
MP4 <bbpart>I0500</bbpart> in <bbpart>pSB2K3</bbpart> AraC pBAD (clones 1 + 2) <partinfo>I0500 SpecifiedComponents</partinfo> July 16
MP5 <bbpart>BBa_B0030</bbpart> in <bbpart>pSB1A2</bbpart> Strong RBS (clones 1 + 2) <partinfo>B0030 SpecifiedComponents</partinfo> July 18
MP6 <bbpart>BBa_E0422</bbpart> in <bbpart>pSB1A2</bbpart> ECFP (RBS+LVA+Term) (clones 1 + 2) <partinfo>E0422 SpecifiedComponents</partinfo> MP6'.1, MP6'.2 July 18, July 24
MP7 <bbpart>BBa_E0241</bbpart> in <bbpart>pSB1A2</bbpart> PoPs to GFP converter (clones 1 + 2) <partinfo>BBa_E0241 DeepComponents</partinfo> MP7'.1 July 18, July 24
MP8 <bbpart>BBa_E0840</bbpart> in <bbpart>pSB1A2</bbpart> gfp tri-part; strong rbs (clones 1 + 2) <partinfo>E0840 SpecifiedComponents</partinfo> July 18
MP9 <bbpart>BBa_J61047</bbpart> in <bbpart>pSB1A2</bbpart> Cre ORF (clones 1 + 2) <partinfo>J61047 SpecifiedComponents</partinfo> MP9'.1, MP9'.2 July 18, July 24
MP10 <bbpart>BBa_I0500</bbpart> in <bbpart>pSB2K3</bbpart> AraC pBAD (clones 1 + 2) <partinfo>I0500 SpecifiedComponents</partinfo> July 20
MP11 <bbpart>BBa_pJ23107</bbpart> in <bbpart>p61002</bbpart> Medium constitutive promoter in J61002 (clones 3 + 4) <partinfo>pJ23107 SpecifiedComponents</partinfo> MP11'.3 July 20, July 23
MP12 <bbpart>BBa_P1003</bbpart> in <bbpart>pSB1A2</bbpart> KanR <partinfo>P1003 SpecifiedComponents</partinfo> July 27
MP13 <bbpart>BBa_J61002</bbpart> ptet-mRFP <partinfo>J61002 SpecifiedComponents</partinfo> MP13.1, MP13.2 August 14
MP14 <bbpart>BBa_J61025</bbpart> Medium constitutive promoter in J61002 (clones 1 + 2) <partinfo>BBa_J61025 SpecifiedComponents</partinfo> MP14.1, MP14.2 August 14
L1 <bbpart>BBa_I0500</bbpart> in <bbpart>J61002</bbpart> construct with pBad promotor regulating expression of mRFP <partinfo>I0500 SpecifiedComponents</partinfo> L1".1, L1".2 August 14
AttB MP15 August 14
AttP Finished MP16 July 25, July 27, Aug 7
L2 Lox66DapAE.coli in <bbpart>pSB1A2</bbpart> Insertion of lox66DapAE.coli construct as a biobrick in pSB1A2 plasmid

http://parts.mit.edu/registry/images/9/9f/Part_icon_dna.png http://parts.mit.edu/registry/images/7/7b/Part_icon_cds.png

July 25
L6 <bbpart>BBa_B0030</bbpart>+<bbpart>BBa_J61047</bbpart> strong RBS(B0030)-Cre ORF

http://parts.mit.edu/r/parts/images/partbypart/icon_rbs.png http://parts.mit.edu/registry/images/7/7b/Part_icon_cds.png

L6.1, L6.2, L6.3, L6".1, L6".2 July 25, July 27
L9 lox66 in <bbpart>pSB1A2</bbpart> July 25, July 30
L10 lox71 in <bbpart>pSB1A2</bbpart> July 25, July 30
L12 lox71-gfp in pSB1A2 construction of the biobrick lox71-gfp

http://parts.mit.edu/registry/images/9/9f/Part_icon_dna.png http://parts.mit.edu/r/parts/images/partbypart/icon_reporter.png

L12.1 & L12.2 July 30
L26 araC/pbad>>RBS-Cre in <bbpart>J61002</bbpart> ligation ( Aug.13) of RBS-Cre as a CDS behind pbad in <bbpart>J61002</bbpart> L26.1 (no more) & L26.2 Aug. 17
L29 gfptripart-lox66 <bbpart>pSB1A2</bbpart> ligation ( Aug.13) of gfp-tripart in front of lox66 L29.1 & L29.2 Aug. 17
L30 dapAp>>mRFP in <bbpart>J61002</bbpart> dapAp promotor placed( Aug.13) upstream of mRFP <bbpart>J61002</bbpart> L30A1 & L30A2 Aug. 17
L31 lox71-<bbpart>B0015</bbpart>(T) in <bbpart>pSB1A2</bbpart> L31.1 & L31.3 Aug. 20
L32 <bbpart>B0015</bbpart>(T)-lox66 in <bbpart>pSB1A2</bbpart> L32.1 & L32.2 Aug. 20
L34 pTet>>lox71-gfptripart in <bbpart>pSB1A2</bbpart> L34.1 & L34.2 Aug. 20
L35 araC/pBad>>lox71-gfptripart in <bbpart>pSB1A2</bbpart> il manque la seq. de 740-1410 L35.1 & L35.2 Aug. 20
L36 lox66-mRFP L36.1 & L36.2 Aug. 20
L37 lox66-RBS-dapAcoli L37.1 & L37.2 Aug. 20
L39 RBS-dapAcoli cloned in <bbpart>pSB1A2</bbpart> L39.1 & L39.2 Aug. 20
L46 pTet>>lox71-B0015(T) in <bbpart>pSB1A2</bbpart> L46.1 Sep. 18
L53 B0015(T)-lox66-RBSDapAcoli in <bbpart>pSB1A2</bbpart> L53.7 Sep. 18
L56 B0015(T)-lox66-RBSdapAsubtilis in <bbpart>pSB1A2</bbpart> L56.3 (attention, seq a partiellement foiré, refaire seq) Sep. 18
L59 pTet>>lox71-gfptripart-lox66-mRFP <bbpart>pSB1A2</bbpart> L59.6 (attention, seq avec O40 a foiré, mais les extrémités sont bonnes, je pense que c ok car les colonies sont vertes) Sep. 18
L64 RBSdapAsubtilis cloned in <bbpart>pSB1A2</bbpart> L564.1 & L64.2 Sep. 18
MPT1 Lox66-DapAColi pTOPO P5 MPT1.1, MPT1.2 Aug 7
MPT3 Lox66-DapASubtilis pTOPO P6 MPT3.2, MPT3.1 Aug 7
MPT4 RBS-DapAColi pTOPO P8 MPT4.1, .2, .3, .4& .5 Aug 7
MPT5 RBS-DapASubtilis pTOPO P9 MPT5.1, .2 &.3 Aug 7
MP15 pKD46 Oct 2
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