Alberta NINT/16 July 2008

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(New page: < Back to notebook < Previous entry lab work (SD): Since the double-plasmid cell cultures failed to grow on ...)
Line 17: Line 17:
             K102035 (LB+kan35)
             K102035 (LB+kan35)
             K102036 (LB+kan35)
             K102036 (LB+kan35)
 +
 +
lab work (JD):
 +
            Mini-prep of pSB4A5, K102003, K102006
 +
            Restriction digests: pSB4A5/E+P, K102003/E+P and K102006/E+P
 +
            Ran gel electrophoresis, 2% gel, 100V
 +
           
[[Alberta_NINT/17_July_2008 | Next entry >]]
[[Alberta_NINT/17_July_2008 | Next entry >]]

Revision as of 17:51, 21 July 2008

< Back to notebook

< Previous entry

lab work (SD): 

            Since the double-plasmid cell cultures failed to grow on the LB+amp50+kan35 plates we will try to 
            grow them on LB+kan35.  Each of the plasmids is also transformed alone into XL1-B cells and plated 
            on kan35 plates as well. 
            K102022 (LB+amp50)
            K102032 (LB+amp50)
            K102032+K102011 (LB+kan35)
            K102032+K102034 (LB+kan35)
            K102032+K102035 (LB+kan35)
            K102032+K102036 (LB+kan35)
            K102011 (LB+kan35)
            K102034 (LB+kan35)
            K102035 (LB+kan35)
            K102036 (LB+kan35)

lab work (JD): 

            Mini-prep of pSB4A5, K102003, K102006
            Restriction digests: pSB4A5/E+P, K102003/E+P and K102006/E+P
            Ran gel electrophoresis, 2% gel, 100V

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