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Team:Paris/Notebook/Freezer
From 2008.igem.org
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| style="background: #ccffcc;" |MP3 | | style="background: #ccffcc;" |MP3 | ||
|[http://partsregistry.org/Part:BBa_B0015 B0015] in [http://partsregistry.org/Part:BBa_SB1Ak3 pSB1AK3] | |[http://partsregistry.org/Part:BBa_B0015 B0015] in [http://partsregistry.org/Part:BBa_SB1Ak3 pSB1AK3] | ||
| - | |Double terminator (clones 1 + 2) [[Image:Part_icon_terminator.png|B0015]] | + | |Double terminator (clones 1 + 2) [[Image:Part_icon_terminator.png| B0015]][[Image:Part_icon_terminator.png| B0015]] |
|MP3.3, MP3.4 | |MP3.3, MP3.4 | ||
| [[Paris/August 14 2007| August 14, 2007]] | | [[Paris/August 14 2007| August 14, 2007]] | ||
| Line 224: | Line 224: | ||
| style="background: #ccffcc;" |MP4 | | style="background: #ccffcc;" |MP4 | ||
|[http://partsregistry.org/Part:BBa_I0500 I0500] in [http://partsregistry.org/Part:BBa_SB2K3 pSB2K3] | |[http://partsregistry.org/Part:BBa_I0500 I0500] in [http://partsregistry.org/Part:BBa_SB2K3 pSB2K3] | ||
| - | |AraC pBAD (clones 1 + 2) [[Image:Part_icon_regulatory.png|pJ23100]] | + | |AraC pBAD (clones 1 + 2) [[Image:Part_icon_regulatory.png | pJ23100]] |
| | | | ||
| - | | [[Paris/July 16| July 16]] | + | | [[Paris/July 16| July 16, 2007]] |
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |MP5 | | style="background: #ccffcc;" |MP5 | ||
|[http://partsregistry.org/Part:BBa_B0030 B0030] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2] | |[http://partsregistry.org/Part:BBa_B0030 B0030] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2] | ||
| - | |Strong RBS (clones 1 + 2) | + | |Strong RBS (clones 1 + 2) [[Image:Part_icon_rbs.png |B0030]] |
| | | | ||
| - | | [[Paris/July 18| July 18]] | + | | [[Paris/July 18| July 18, 2007]] |
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |MP6 | | style="background: #ccffcc;" |MP6 | ||
| Line 238: | Line 238: | ||
|ECFP (RBS+LVA+Term) (clones 1 + 2) <partinfo>E0422 SpecifiedComponents</partinfo> | |ECFP (RBS+LVA+Term) (clones 1 + 2) <partinfo>E0422 SpecifiedComponents</partinfo> | ||
|MP6'.1, MP6'.2 | |MP6'.1, MP6'.2 | ||
| - | | [[Paris/July 18| July 18]] | + | | [[Paris/July 18| July 18, 2007]]; [[Paris/July 24| July 24, 2007]] |
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |MP7 | | style="background: #ccffcc;" |MP7 | ||
| Line 244: | Line 244: | ||
|PoPs to GFP converter (clones 1 + 2) <partinfo>BBa_E0241 DeepComponents</partinfo> | |PoPs to GFP converter (clones 1 + 2) <partinfo>BBa_E0241 DeepComponents</partinfo> | ||
|MP7'.1 | |MP7'.1 | ||
| - | | [[Paris/July 18| July 18]] | + | | [[Paris/July 18| July 18, 2007]]; [[Paris/July 24| July 24, 2007]] |
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |MP8 | | style="background: #ccffcc;" |MP8 | ||
Revision as of 12:27, 22 July 2008
PCR Products
| PCR products | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Number | Name | Oligo F | Oligo R | Matrix | Length | Comments | Product Left ? | []ng/µL | Date |
| P1 | Lox71-FtsA-FtsZ-1 | O3 Lox71-FtsA-F | O4 DEcoR1-FtsZ-R | MG1655 | 1537 | Ø | |||
| P2 | FtsZ-2 | O5 DEcoR1-FtsZ-F | O2 FtsZ-R | MG1655 | 1088 | Ø | |||
| P3 | DGAT-1 | 27 ForDGAT1 | 13 DPst1-DGAT-R | Miniprep pKS::DGAT | 1087 | July 31 | |||
| P4 | DGAT-2 | 12 DPst1-DGAT | 27 Rev-DGAT1 | Miniprep pKS::DGAT | 396 | July 31 | |||
| P5 | Lox66-DapAColi | O6 Lox66-DapAColi-F | O7 DapAColi-R | MG1655 | 985 | Ø | July 17, July 30 | ||
| P6 | Lox66-DapASubtilis | O8 Lox66-DapASubt-F | O9 DapASubt-R | B.Subtilis 168 | 979 | July 17, July 27, July 30 | |||
| P7 | Lox71-FtsZ | O1 Lox71-FtsZ-F | O2 FtsZ-R | MG1655 | 1260 | EcoRI site in FtsZ not mutated | July 17, July 30 | ||
| P8 | RBS-DapAColi | O20 RBS-DapAColi-F | O7 DapAColi-R | MG1655 | 952 | B0030-DapAColi | July 23, July 30 | ||
| P9 | RBS-DapASubtilis | O21 RBS-DapASubt-F | O9 DapASubt-R | B.Subtilis 168 | 946 | B0030-DapASubtilis | July 23, July 27 | ||
| P10 | Lox71-FtsA-FtsZ | O3 Lox71-FtsA-F | O2 FtsZ-R | MG1655 | 2580 | EcoRI site in FtsZ not mutated | July 23 | ||
| P11 | Lox71-KmR-Lox66 | O10 Lox71-KmR-F | O11 Lox66-KmR-R | MP12 | 1084 | July 27, July 30 | |||
| P13 | DGAT deltaPst-1 | ForDGAT1 | Megaprimer DGAT2 | DNA plasmid (pKS::DGAT): | 1443 | August 1 | |||
| P14 | DGAT deltaPst-1 biobrick | ForDGAT1bb | RevDGAT1bb | DGAT deltaPst1 | 1504 | August 2 Oct. 18 | |||
| P15 | PacI [pTet>>lox-GFP-loxmRFP] XmaI | O47 | O48 | [pTet>>lox-GFP-loxmRFP] L59.6 | 2kb | August 2 | |||
| P16 | PrefixBB-frt1-CmR-frt2-SufixBB | O51 | O52 | pY-2C-IntC | 1kb | Oct. 15 | |||
| P17 | PrefixBB-frt1-CmR-frt2-T1T2-SufixBB | O51 | O53 | pY-2C-IntC | >1kb | Oct. 18 | |||
Plasmids : MiniPrep Products
| Plasmids: MiniPrep Products | ||||
|---|---|---|---|---|
| Number | Name | Description | Products in use | Date |
| MP1 | [http://partsregistry.org/Part:BBa_J23100 pJ23100] in [http://partsregistry.org/Part:BBa_J61002 J61002] | Strong constitutive promoter in J61002(clones 1 + 2) 
| July 15, 2007; Aug. 17, 2007 | |
| MP2 | [http://partsregistry.org/Part:BBa_J23107 pJ23107] in [http://partsregistry.org/Part:BBa_J61002 J61002] | Medium constitutive promoter in J61002 (clones 1 + 2)
| MP2.2 | July 15, 2007; Aug. 17, 2007 |
| MP3 | [http://partsregistry.org/Part:BBa_B0015 B0015] in [http://partsregistry.org/Part:BBa_SB1Ak3 pSB1AK3] | Double terminator (clones 1 + 2) 
| MP3.3, MP3.4 | August 14, 2007 |
| MP4 | [http://partsregistry.org/Part:BBa_I0500 I0500] in [http://partsregistry.org/Part:BBa_SB2K3 pSB2K3] | AraC pBAD (clones 1 + 2)
| July 16, 2007 | |
| MP5 | [http://partsregistry.org/Part:BBa_B0030 B0030] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2] | Strong RBS (clones 1 + 2) 
| July 18, 2007 | |
| MP6 | [http://partsregistry.org/Part:BBa_E0422 E0422] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2] | ECFP (RBS+LVA+Term) (clones 1 + 2) <partinfo>E0422 SpecifiedComponents</partinfo> | MP6'.1, MP6'.2 | July 18, 2007; July 24, 2007 |
| MP7 | <bbpart>BBa_E0241</bbpart> in <bbpart>pSB1A2</bbpart> | PoPs to GFP converter (clones 1 + 2) <partinfo>BBa_E0241 DeepComponents</partinfo> | MP7'.1 | July 18, 2007; July 24, 2007 |
| MP8 | <bbpart>BBa_E0840</bbpart> in <bbpart>pSB1A2</bbpart> | gfp tri-part; strong rbs (clones 1 + 2) <partinfo>E0840 SpecifiedComponents</partinfo> | July 18 | |
| MP9 | <bbpart>BBa_J61047</bbpart> in <bbpart>pSB1A2</bbpart> | Cre ORF (clones 1 + 2) <partinfo>J61047 SpecifiedComponents</partinfo> | MP9'.1, MP9'.2 | July 18, July 24 |
| MP10 | <bbpart>BBa_I0500</bbpart> in <bbpart>pSB2K3</bbpart> | AraC pBAD (clones 1 + 2) <partinfo>I0500 SpecifiedComponents</partinfo> | July 20 | |
| MP11 | <bbpart>BBa_pJ23107</bbpart> in <bbpart>p61002</bbpart> | Medium constitutive promoter in J61002 (clones 3 + 4) <partinfo>pJ23107 SpecifiedComponents</partinfo> | MP11'.3 | July 20, July 23 |
| MP12 | <bbpart>BBa_P1003</bbpart> in <bbpart>pSB1A2</bbpart> | KanR <partinfo>P1003 SpecifiedComponents</partinfo> | July 27 | |
| MP13 | <bbpart>BBa_J61002</bbpart> | ptet-mRFP <partinfo>J61002 SpecifiedComponents</partinfo> | MP13.1, MP13.2 | August 14 |
| MP14 | <bbpart>BBa_J61025</bbpart> | Medium constitutive promoter in J61002 (clones 1 + 2) <partinfo>BBa_J61025 SpecifiedComponents</partinfo> | MP14.1, MP14.2 | August 14 |
| L1 | <bbpart>BBa_I0500</bbpart> in <bbpart>J61002</bbpart> | construct with pBad promotor regulating expression of mRFP <partinfo>I0500 SpecifiedComponents</partinfo> | L1".1, L1".2 | August 14 |
| AttB | MP15 | August 14 | ||
| AttP | Finished | MP16 | July 25, July 27, Aug 7 | |
| L2 | Lox66DapAE.coli in <bbpart>pSB1A2</bbpart> | Insertion of lox66DapAE.coli construct as a biobrick in pSB1A2 plasmid
http://parts.mit.edu/registry/images/9/9f/Part_icon_dna.png http://parts.mit.edu/registry/images/7/7b/Part_icon_cds.png | July 25 | |
| L6 | <bbpart>BBa_B0030</bbpart>+<bbpart>BBa_J61047</bbpart> | strong RBS(B0030)-Cre ORF
http://parts.mit.edu/r/parts/images/partbypart/icon_rbs.png http://parts.mit.edu/registry/images/7/7b/Part_icon_cds.png | L6.1, L6.2, L6.3, L6".1, L6".2 | July 25, July 27 |
| L9 | lox66 in <bbpart>pSB1A2</bbpart> | July 25, July 30 | ||
| L10 | lox71 in <bbpart>pSB1A2</bbpart> | July 25, July 30 | ||
| L12 | lox71-gfp in pSB1A2 | construction of the biobrick lox71-gfp
http://parts.mit.edu/registry/images/9/9f/Part_icon_dna.png http://parts.mit.edu/r/parts/images/partbypart/icon_reporter.png | L12.1 & L12.2 | July 30 |
| L26 | araC/pbad>>RBS-Cre in <bbpart>J61002</bbpart> | ligation ( Aug.13) of RBS-Cre as a CDS behind pbad in <bbpart>J61002</bbpart> | L26.1 (no more) & L26.2 | Aug. 17 |
| L29 | gfptripart-lox66 <bbpart>pSB1A2</bbpart> | ligation ( Aug.13) of gfp-tripart in front of lox66 | L29.1 & L29.2 | Aug. 17 |
| L30 | dapAp>>mRFP in <bbpart>J61002</bbpart> | dapAp promotor placed( Aug.13) upstream of mRFP <bbpart>J61002</bbpart> | L30A1 & L30A2 | Aug. 17 |
| L31 | lox71-<bbpart>B0015</bbpart>(T) in <bbpart>pSB1A2</bbpart> | L31.1 & L31.3 | Aug. 20 | |
| L32 | <bbpart>B0015</bbpart>(T)-lox66 in <bbpart>pSB1A2</bbpart> | L32.1 & L32.2 | Aug. 20 | |
| L34 | pTet>>lox71-gfptripart in <bbpart>pSB1A2</bbpart> | L34.1 & L34.2 | Aug. 20 | |
| L35 | araC/pBad>>lox71-gfptripart in <bbpart>pSB1A2</bbpart> | il manque la seq. de 740-1410 | L35.1 & L35.2 | Aug. 20 |
| L36 | lox66-mRFP | L36.1 & L36.2 | Aug. 20 | |
| L37 | lox66-RBS-dapAcoli | L37.1 & L37.2 | Aug. 20 | |
| L39 | RBS-dapAcoli cloned in <bbpart>pSB1A2</bbpart> | L39.1 & L39.2 | Aug. 20 | |
| L46 | pTet>>lox71-B0015(T) in <bbpart>pSB1A2</bbpart> | L46.1 | Sep. 18 | |
| L53 | B0015(T)-lox66-RBSDapAcoli in <bbpart>pSB1A2</bbpart> | L53.7 | Sep. 18 | |
| L56 | B0015(T)-lox66-RBSdapAsubtilis in <bbpart>pSB1A2</bbpart> | L56.3 (attention, seq a partiellement foiré, refaire seq) | Sep. 18 | |
| L59 | pTet>>lox71-gfptripart-lox66-mRFP <bbpart>pSB1A2</bbpart> | L59.6 (attention, seq avec O40 a foiré, mais les extrémités sont bonnes, je pense que c ok car les colonies sont vertes) | Sep. 18 | |
| L64 | RBSdapAsubtilis cloned in <bbpart>pSB1A2</bbpart> | L564.1 & L64.2 | Sep. 18 | |
| MPT1 | Lox66-DapAColi | pTOPO P5 | MPT1.1, MPT1.2 | Aug 7 |
| MPT3 | Lox66-DapASubtilis | pTOPO P6 | MPT3.2, MPT3.1 | Aug 7 |
| MPT4 | RBS-DapAColi | pTOPO P8 | MPT4.1, .2, .3, .4& .5 | Aug 7 |
| MPT5 | RBS-DapASubtilis | pTOPO P9 | MPT5.1, .2 &.3 | Aug 7 |
| MP15 | pKD46 | Oct 2 | ||
