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Team:Paris/Notebook/Freezer
From 2008.igem.org
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| - | + | == Digestion Products == | |
<br> | <br> | ||
<font color="#FF99FF" size="4">PURPLE = Vector </font><br> | <font color="#FF99FF" size="4">PURPLE = Vector </font><br> | ||
Revision as of 15:32, 22 July 2008
PCR products
| PCR products | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Number | Name | Oligo F | Oligo R | Matrix | Length | Comments | Product Left ? | []ng/µL | Date |
| [[ ]] | |||||||||
| [[ ]] | |||||||||
Plasmids: MiniPrep Products
| Plasmids: MiniPrep Products | ||||
|---|---|---|---|---|
| Number | Name | Description | Products in use | Date |
| MP1 | [http://partsregistry.org/Part:BBa_J23100 pJ23100] in [http://partsregistry.org/Part:BBa_J61002 J61002] | Strong constitutive promoter in J61002 (clones 1 + 2) 
| 2007 | |
| MP2 | [http://partsregistry.org/Part:BBa_J23107 pJ23107] in [http://partsregistry.org/Part:BBa_J61002 J61002] | Medium constitutive promoter in J61002 (clones 1 + 2)
| MP2.2 | 2007 |
| MP3 | [http://partsregistry.org/Part:BBa_B0015 B0015] in [http://partsregistry.org/Part:BBa_SB1AK3 pSB1AK3] | Double terminator (clones 1 + 2) 
| MP3.3, MP3.4 | 2007 |
| MP4 | [http://partsregistry.org/Part:BBa_I0500 I0500] in [http://partsregistry.org/Part:BBa_SB2K3 pSB2K3] | AraC pBAD (clones 1 + 2)
| 2007 | |
| MP5 | [http://partsregistry.org/Part:BBa_B0030 B0030] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2] | Strong RBS (clones 1 + 2) 
| 2007 | |
| MP6 | [http://partsregistry.org/Part:BBa_E0422 E0422] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2] | ECFP (RBS+LVA+Term) (clones 1 + 2) 
| MP6'.1, MP6'.2 | 2007 |
| MP8 | [http://partsregistry.org/Part:BBa_E0840 E0840] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2] | gfp tri-part; strong rbs (clones 1 + 2)
| 2007 | |
Digestion Products
PURPLE = Vector
GREEN = Insert
| Digestion Products | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Number | Product Name | Matrix Name | Enzyme 1 | Enzyme 2 | Size | Description | Product Left | []ng/µL | Date |
| D1 | <bbpart>pJ23100</bbpart> | <bbpart>pJ23100</bbpart> | Spe1 | Pst1 | 2096 | Vector with the J23100 promoter ready for insertion of a RBS/CDS insert | Ø (24/09/07) | 34 | July 17 |
| D2 | <bbpart>mRFP dblTer</bbpart> | <bbpart>pJ23100</bbpart> | Spe1 | Pst1 | 887 | 27 | July 17 | ||
| D3 | <bbpart>pJ23107</bbpart> | <bbpart>pJ23107</bbpart> | Spe1 | Pst1 | 2096 | Vector with the J23107 promoter ready for insertion of a RBS/CDS insert | Ø (24/09/07) | 32 | July 17 |
| D4 | <bbpart>mRFP dblTer</bbpart> | <bbpart>pJ23107</bbpart> | Spe1 | Pst1 | 887 | 25 | July 17 | ||
| D5 | <bbpart>pJ23100</bbpart> | <bbpart>pJ23100</bbpart> | EcoR1 | Spe1 | 2925 | The vector with the mRFP gene ready for insertion of a promoter. We are not sure the digestion worked properly. | 28&29 | July 17 | |
| D6 | <bbpart>pJ23107</bbpart> | <bbpart>pJ23107</bbpart> | EcoR1 | Spe1 | 2925 | The vector with the mRFP gene ready for insertion of a promoter. We are not sure the digestion worked properly. | 31&27 | July 17 | |
| D7 | Lox66 DapA E.coli | Lox66 DapA E.coli (PCR product) | EcoR1 | Pst1 | 977 | 24 | July 17 | ||
| D8 | Lox66 DapA E.coli | Lox66 DapA E.coli (PCR product) | Xba1 | Pst1 | 962 | 44 | July 17 | ||
| D9 | <bbpart>pJ23100</bbpart> ES vector | <bbpart>pJ23100</bbpart> (MP1.1 & MP1.2) | EcoI | SpeI | 2925 | Vector with mRFP RBS/CDS ready for insertion promoter (fw-insert) | 80&43 | July 21 | |
| D10 | <bbpart>pJ23100</bbpart> promoter fw-insert | <bbpart>pJ23100</bbpart> (MP1.1 & MP1.2) | EcoI | SpeI | 58 | J23100 promoter ready for use as a fw-insert | 35&28 | July 21 | |
| D11 | <bbpart>pJ23107</bbpart> ES vector | <bbpart>pJ23107</bbpart> (MP2.1, MP2.2, MP2.3 & MP2.4) | EcoRI | SpeI | 2925 | Vector with mRFP RBS/CDS ready for insertion of a fw-insert promoter | 49,35,28&41 | July 21 | |
| D12 | <bbpart>pJ23107</bbpart> promoter fw-insert | <bbpart>pJ23107</bbpart> (MP2.1, MP2.2, MP2.3 & MP2.4) | EcoRI | SpeI | 58 | J23107 promoter ready for usage as a fw-insert | 23,25,32&32 | July 21 | |
| D13 | <bbpart>mRFP</bbpart> | <bbpart>pJ23107</bbpart> (MP2.2, MP2.3 & MP2.4) | SpeI | PstI | 887 | mRFP RBS/CDS/T sequence, can it be used as a backward insert? => yes but you will not be able to add any biobrick after it | 21&41 | July 21 | |
| D14 | Cre ORF | <bbpart>J61047</bbpart> MP9.1 & MP9.2 | XbaI | PstI | 1063 | Cre ORF extracted for usage as a bw-insert | Ø D14.1 | 38&41 | July 21 |
| D15 | <bbpart>B0030</bbpart> bw-vector | <bbpart>B0030</bbpart> MP5.1 & MP5.2 | SpeI | PstI | 2076 | bacward vector for cloning a CDS bw-insert after the B0030 RBS | 87&42 | July 21 | |
| D18 | BB dig. lox66DapAE.coli | lox66-DapAE.coli P5 PCR product | EcoRI | PstI | ~960 | PCR product digested for cloning as a BioBrick | Ø | 44 | July 21 |
| D19 | BB dig. lox66DapAsubtilis | lox66-DapAsubtilis P6 PCR product | EcoRI | PstI | ~960 | PCR product digested for cloning as a BioBrick | Ø | July 21 | |
| D19' | BB dig. lox66DapAsubtilis | lox66-DapAsubtilis P6' PCR product | EcoRI | PstI | ~960 | PCR product digested for cloning as a BioBrick | 16 | July 30 | |
| D20 | bw-insert lox66-DapAE.coli | lox66-DapAE.coli P5 PCR product | XbaI | PstI | ~960 | PCR product digested for usage as a backward insert | Ø | July 21 | |
| D21 | bw-insert lox66-DapAsubtilis | lox66-DapAsubtilis P6 PCR product | XbaI | PstI | ~960 | PCR product digested for usage as a backward insert | Ø | July 21 | |
| D21' | bw-insert lox66-DapAsubtilis | lox66-DapAsubtilis P6' PCR product | XbaI | PstI | ~960 | PCR product digested for cloning as a BI | 34 | July 30 | |
| D22 | pSB1A2 open vector | <bbpart>BBa_J61047</bbpart> (MP9.1 & MP9.2) | EcoI | PstI | Ø D22.1 | Open pSB1A2 vector for BioBrick EcoRI/PstI insertio | Ø (24/09/07) | D22.2:59 | July 23, Aug. 13 |
| D23 | AraC/pBad promoter fw-insert | <bbpart>BBa_I0500</bbpart> (MP4.1 & MP4.2) | EcoI | SpeI | 1233 | AraC/pBad promoter ready for use as a forward insert | Ø D23.1 | 93&53 | July 23 |
| D24 | eCFP bw-insert | <bbpart>BBa_E0422</bbpart> (MP6.1 & MP6.2) | XbaI | PstI | eCFP (RBS-OFR_LVA-T) ready for use as a backward insert | 56&55 | July 23 | ||
| D26 | gfp-tripart | <bbpart>BBa_E0840</bbpart> (MP8.1 & MP8.2) | XbaI | PstI | gfp-tri part (strongRBS-ORF-T) ready for use as a backward insert | 47&47 | July 23 | ||
| D27 | lox71-ftsZ bw-insert | lox71-ftsZ PCR product P7 | XbaI | PstI | lox71-ftsZ(unmutated) ready for use as a backward insert | Ø | July 23 | ||
| D27' | lox71-ftsZ bw-insert | lox71-ftsZ PCR product P7 | XbaI | PstI | lox71-ftsZ(unmutated) ready for use as a backward insert | 24 | July 30 | ||
| D28 | RBS-DapAColi BB | P8 (RBS-DapAColi) | EcoRI | PstI | ~950 | Biobrick | 54 | July 25 | |
| D29 | RBS-DapAColi | P8 (RBS-DapAColi) | XbaI | PstI | ~950 | Backward insert | 44 | July 25 | |
| D30 | RBS-DapASubtilis BB | P9 (RBS-DapASubtilis) | EcoRI | PstI | ~950 | Biobrick | 72 | July 25 | |
| D31 | RBS-DapASubtilis | P9 (RBS-DapASubtilis) | XbaI | PstI | ~950 | Backward insert | 51 | July 25 | |
| D32 | Lox71-FtsA-FtsZ | P10 Lox71-FtsA-FtsZ | XbaI | PstI | ~2600 | Backward insert - EcoRI site still presents : we can't use EcoRI | 47 | July 25 | |
| D33 | RBS(B0030)CreORF | L6' (clone 1 & 2) | XbaI | PstI | RBS-Cre digestion for use as a BI | 29&27 | July 30, August 13 | ||
| D34 | lox66 | L9' lox66 | SpeI | PstI | Biobrick plasmid carrying lox66 digested as a BV | 18 | July 30 | ||
| D35 | lox71 | L10' lox71 | SpeI | PstI | Biobrick plasmid carrying lox71 digested as a BV | July 30, August 13 | |||
| D36 | lox71-KmR-lox66 | P11 lox71-KmR-lox66 | EcoRI | PstI | lox71-KmR-lox66 digested as a biobrick insert | July 30 | |||
| D37 | lox71-gfp(tripart) BI | L12.1 & L12.2 miniprep products (from 30/07 ligation reaction) | XbaI | PstI | lox71_RBS-gfp-double terminator extracted as a backward insert | Aug. 6 | |||
| D38 | BB-insert lox66-DapAE.coli | lox66-DapAE.coli in pTOPO (P5 PCR product)(clones 1+2) | EcoRI | PstI | lox66-dapAE.coli extracted from pTOPO plasmid as a BB insert | Aug. 6 | |||
| D39' | BI-insert lox66-DapAE.coli | lox66-DapAE.coli in pTOPO (P5 PCR product)(clones 1+2) | XbaI | PstI | lox66-dapAcoli extracted for usage as a backward insert | Aug. 7 | |||
| D40 | BB-insert lox71-KmR-lox66 | L25.1 miniprep product (from ligation rxn of 30/07 | EcoRI | PstI | lox71-KmR-lox66 construct extracted for clonong as a biobrick | Aug. 6 | |||
| D41 | araC/pBad-J61002 plasmid SP dig. | L1.2, L1".1 & L1".2 miniprep product | SpeI | PstI | J61002 plasmid containing araC/pBad promotor digested for insertion of a BI CDS | Aug. 13 | |||
| D42 | Lox66 FV | L9 | EcoRI | XbaI | pSB1A2-lox66 as a Forward vector | August 13 | |||
| D43 | gfp-tripart FI | MP8.1 | EcoRI | SpeI | gfp-tri part (strongRBS-ORF-T) ready for use as a forward insert | August 13 | |||
| D44 | <bbpart>B0015</bbpart> terminator BI | <bbpart>B0015</bbpart> in <bbpart>pSB1AK3</bbpart> (MP3.3 Aug.14) | XbaI | Pst1 | terminator B0015 extracted for use as a BI | August 16 | |||
| D45 | <bbpart>B0015</bbpart> terminator FI | <bbpart>B0015</bbpart> in <bbpart>pSB1AK3</bbpart> (MP3.4 Aug.14) | EcoRI | SpeI | terminator B0015 extracted for use as a FI | August 16 | |||
| D46 | <bbpart>B0015</bbpart> BV | <bbpart>B0015</bbpart> in <bbpart>pSB1AK3</bbpart> (MP3.3 Aug.14) | SpeI | Pst1 | <bbpart>B0015</bbpart> in <bbpart>pSB1AK3</bbpart> plasmid extracted for use as a BV | August 16 | |||
| D47 | <bbpart>pSB1A2</bbpart> XP dig. | <bbpart>pSB1A2</bbpart> carrying Cre ORF (MP9.1) | XbaI | PstI | <bbpart>pSB1A2</bbpart> open vector ready for cloning of BI | August 16 | |||
| D48 | <bbpart>pSB1A2</bbpart> ES dig. | <bbpart>pSB1A2</bbpart> carrying Cre ORF (MP9.2) | EcoRI | SpeI | <bbpart>pSB1A2</bbpart> open vector ready for cloning of FI | August 16 | |||
| D49 | lox71-gfptripart BI | lox71-gfptripart in <bbpart>pSB1A2</bbpart> (L12.2) | XbaI | Pst1 | lox71-gfptripart extracted for use as a BI | August 16 | |||
| D50 | <bbpart>BBa_J61002</bbpart> pTet SP dig. | <bbpart>BBa_J61002</bbpart> (MP13.1 Aug. 14) | SpeI | PstI | <bbpart>BBa_J61002</bbpart> pTet promoter vector ready for insertion of CDS as a BI | August 16 | |||
| D51 | RBS-dapAcoli BBinsert | pTOPO of RBS-dapAcoli (MPT4.3) | EcoRI | Pst1 | RBS-dapAcoli extracted for cloning as a biobrick | August 16 | |||
| D52 | RBS-dapAcoli BI | pTOPO of RBS-dapAcoli (MPT4.3) | XbaI | Pst1 | RBS-dapAcoli extracted for use as a BI | August 16 | |||
| D53 | RBS-dapAsubtilis BBinsert | pTOPO of RBS-dapAsubtilis (MPT5.1) | EcoRI | Pst1 | RBS-dapAsubtilis extracted for cloning as a biobrick | August 16 | |||
| D54 | RBS-dapAsubtilis BI | pTOPO of RBS-dapAsubtilis (MPT5.1) | XbaI | Pst1 | RBS-dapAsubtilis extracted for use as a BI | Ø (24/09/07) | August 16 | ||
| D55 | RBS-dapAsubtilis FI | pTOPO of RBS-dapAsubtilis (MPT5.2) | EcoRI | SpeI | RBS-dapAsubtilis extracted for use as a FI | August 16 | |||
| D57 | lox71-B0015(T) BI | (L31.1, L31.3) lox71-B0015(T) <bbpart>pSB1A2</bbpart> | XbaI | Pst1 | lox71-B0015(T) extracted for use as a BI <bbpart>pSB1A2</bbpart> | Sep. 14 | |||
| D58 | B0015(T)-lox66 BV | (L32.1, L32.2) B0015(T)-lox66 BV | SpeI | PstI | B0015(T)-lox66 for use as a BV <bbpart>pSB1A2</bbpart> | Sep. 14 | |||
| D59 | pTet>>lox71-gfptripart BI | (L34.1, L34.2) pTet>>lox71-gfptripart <bbpart>BBa_J61002</bbpart> | XbaI | PstI | pTet>>lox71-gfptripart for use as a BI | Sep. 14 | |||
| D61 | lox66-mRFP FV | (L36.1, L36.2) lox66-mRFP | EcoRI | XbaI | lox66-mRFP part (strongRBS-ORF-T) ready for use as a forward vector | Sep. 14 | |||
| D62 | lox66-RBSDapAcoli BI | (L37.1, L37.2) lox66-RBSDapAcoli | XbaI | PstI | lox66-RBSDapAcoli ready for use as a Backward insert | Sep. 14 | |||
| D63 | RBSDapAcoli BI | (L39.1, L39.2) RBSDapAcoli | XbaI | PstI | RBSDapAcoli BI (for insertion behind T-lox66) | Sep. 14 | |||
| D64 | FRT-CmR-FRT <bbpart>BBa_J61025</bbpart> BV | (MP14.1) <bbpart>BBa_J61025</bbpart> | SpeI | PstI | FRT-CmR-FRT <bbpart>BBa_J61025</bbpart> (for backward introduction of a casette for Wanner K insertion) | Sep. 14 | |||
| D65 | FRT-CmR-FRT <bbpart>BBa_J61025</bbpart> FV | (MP14.1) <bbpart>BBa_J61025</bbpart> | EcoR | XbaI | FRT-CmR-FRT <bbpart>BBa_J61025</bbpart> (for forward introduction of a casette for Wanner K insertion) | Sep. 14 | |||
| D68 | attB BV | (MP15.1) | SpeI | PstI | vector containing attB for use as a BV | Sep. 14 | |||
| D69 | gfptripart-lox66 BV | (L29.1) gfptripart-lox66 <bbpart>pSB1A2</bbpart> | SpeI | PstI | Sep. 14 | ||||
| D70 | pTet>>lox71-gfptripart FI | (L34.1, L34.2) pTet>>lox71-gfptripart <bbpart>BBa_J61002</bbpart> | EcoRI | SpeI | pTet>>lox71-gfptripart for use as a FI | Sep. 14 | |||
| D71 | araC/pBad>>lox71-gfptripart FI | L35.1 araC/pBad>>lox71-gfptripart <bbpart>BBa_J61002</bbpart> | EcoRI | SpeI | araC/pBad>>lox71-gfptripart for use as a FI | Ø (24/09/07) | Sep. 14 Sep. 17 | ||
| D72 | <bbpart>pSB2K3</bbpart> BBvector | MP16 <bbpart>pSB2K3</bbpart>::attP | EcoRI | PstI | Low copy number plasmid, BB vector | Sep. 17 | |||
| D73 | <bbpart>pSB2K3</bbpart> ES open vector | MP16 <bbpart>pSB2K3</bbpart>::attP | EcoRI | SpeI | Comparison of digestion efficiency between Pst and Spe in NEB2 | Sep. 17 | |||
| D76 | DGAT BB | P14 DGAT | EcoRI | PstI | DGAT | Ø (24/09/07) | Sep. 17 | ||
| D77 | araC/pBad>>lox71-gfptripart BI | D60 (.1 & .2) digestion product | FspI | AhdI | in D60, the extracted fragment & the open plasmid have the same size, it is thus necessary to remove the plasmid by digestion | Sep. 17 | |||
| D78 | araC/pBad>>lox71-gfptripart FI | D71 (.1 & .2) digestion product | FspI | AhdI | in D71, the extracted fragment & the open plasmid have the same size, it is thus necessary to remove the plasmid by digestion | Sep. 17 | |||
