Team:Paris/Notebook/Freezer

From 2008.igem.org

(Difference between revisions)

Revision as of 15:39, 22 July 2008

PCR products

PCR products
Number	Name	Oligo F	Oligo R	Matrix	Length	Comments	Product Left ?	[]ng/µL	Date
									[[ ]]
									[[ ]]

Plasmids: MiniPrep Products

Plasmids: MiniPrep Products
Number	Name	Biobricks	Description	Products in use	Date
MP1	[http://partsregistry.org/Part:BBa_J23100 pJ23100] in [http://partsregistry.org/Part:BBa_J61002 J61002]		Strong constitutive promoter in J61002 (clones 1 + 2)		2007
MP2	[http://partsregistry.org/Part:BBa_J23107 pJ23107] in [http://partsregistry.org/Part:BBa_J61002 J61002]		Medium constitutive promoter in J61002 (clones 1 + 2)	MP2.2	2007
MP3	[http://partsregistry.org/Part:BBa_B0015 B0015] in [http://partsregistry.org/Part:BBa_SB1AK3 pSB1AK3]		Double terminator (clones 1 + 2)	MP3.3, MP3.4	2007
MP4	[http://partsregistry.org/Part:BBa_I0500 I0500] in [http://partsregistry.org/Part:BBa_SB2K3 pSB2K3]		AraC pBAD (clones 1 + 2)		2007
MP5	[http://partsregistry.org/Part:BBa_B0030 B0030] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2]		Strong RBS (clones 1 + 2)		2007
MP6	[http://partsregistry.org/Part:BBa_E0422 E0422] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2]		ECFP (RBS+LVA+Term) (clones 1 + 2)	MP6'.1, MP6'.2	2007
MP8	[http://partsregistry.org/Part:BBa_E0840 E0840] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2]		gfp tri-part; strong rbs (clones 1 + 2)		2007

Digestion Products

PURPLE = Vector
GREEN = Insert

Digestion Products
Number	Product Name	Matrix Name	Enzyme 1	Enzyme 2	Size	Description	Product Left	[]ng/µL	Date
D1	<bbpart>pJ23100</bbpart>	<bbpart>pJ23100</bbpart>	Spe1	Pst1	2096	Vector with the J23100 promoter ready for insertion of a RBS/CDS insert	Ø (24/09/07)	34	July 17
D2	<bbpart>mRFP dblTer</bbpart>	<bbpart>pJ23100</bbpart>	Spe1	Pst1	887			27	July 17
D3	<bbpart>pJ23107</bbpart>	<bbpart>pJ23107</bbpart>	Spe1	Pst1	2096	Vector with the J23107 promoter ready for insertion of a RBS/CDS insert	Ø (24/09/07)	32	July 17
D4	<bbpart>mRFP dblTer</bbpart>	<bbpart>pJ23107</bbpart>	Spe1	Pst1	887			25	July 17
D5	<bbpart>pJ23100</bbpart>	<bbpart>pJ23100</bbpart>	EcoR1	Spe1	2925	The vector with the mRFP gene ready for insertion of a promoter. We are not sure the digestion worked properly.		28&29	July 17
D6	<bbpart>pJ23107</bbpart>	<bbpart>pJ23107</bbpart>	EcoR1	Spe1	2925	The vector with the mRFP gene ready for insertion of a promoter. We are not sure the digestion worked properly.		31&27	July 17
D7	Lox66 DapA E.coli	Lox66 DapA E.coli (PCR product)	EcoR1	Pst1	977			24	July 17
D8	Lox66 DapA E.coli	Lox66 DapA E.coli (PCR product)	Xba1	Pst1	962			44	July 17
D9	<bbpart>pJ23100</bbpart> ES vector	<bbpart>pJ23100</bbpart> (MP1.1 & MP1.2)	EcoI	SpeI	2925	Vector with mRFP RBS/CDS ready for insertion promoter (fw-insert)		80&43	July 21
D10	<bbpart>pJ23100</bbpart> promoter fw-insert	<bbpart>pJ23100</bbpart> (MP1.1 & MP1.2)	EcoI	SpeI	58	J23100 promoter ready for use as a fw-insert		35&28	July 21
D11	<bbpart>pJ23107</bbpart> ES vector	<bbpart>pJ23107</bbpart> (MP2.1, MP2.2, MP2.3 & MP2.4)	EcoRI	SpeI	2925	Vector with mRFP RBS/CDS ready for insertion of a fw-insert promoter		49,35,28&41	July 21
D12	<bbpart>pJ23107</bbpart> promoter fw-insert	<bbpart>pJ23107</bbpart> (MP2.1, MP2.2, MP2.3 & MP2.4)	EcoRI	SpeI	58	J23107 promoter ready for usage as a fw-insert		23,25,32&32	July 21
D13	<bbpart>mRFP</bbpart>	<bbpart>pJ23107</bbpart> (MP2.2, MP2.3 & MP2.4)	SpeI	PstI	887	mRFP RBS/CDS/T sequence, can it be used as a backward insert? => yes but you will not be able to add any biobrick after it		21&41	July 21
D14	Cre ORF	<bbpart>J61047</bbpart> MP9.1 & MP9.2	XbaI	PstI	1063	Cre ORF extracted for usage as a bw-insert	Ø D14.1	38&41	July 21
D15	<bbpart>B0030</bbpart> bw-vector	<bbpart>B0030</bbpart> MP5.1 & MP5.2	SpeI	PstI	2076	bacward vector for cloning a CDS bw-insert after the B0030 RBS		87&42	July 21
D18	BB dig. lox66DapAE.coli	lox66-DapAE.coli P5 PCR product	EcoRI	PstI	~960	PCR product digested for cloning as a BioBrick	Ø	44	July 21
D19	BB dig. lox66DapAsubtilis	lox66-DapAsubtilis P6 PCR product	EcoRI	PstI	~960	PCR product digested for cloning as a BioBrick	Ø		July 21
D19'	BB dig. lox66DapAsubtilis	lox66-DapAsubtilis P6' PCR product	EcoRI	PstI	~960	PCR product digested for cloning as a BioBrick		16	July 30
D20	bw-insert lox66-DapAE.coli	lox66-DapAE.coli P5 PCR product	XbaI	PstI	~960	PCR product digested for usage as a backward insert	Ø		July 21
D21	bw-insert lox66-DapAsubtilis	lox66-DapAsubtilis P6 PCR product	XbaI	PstI	~960	PCR product digested for usage as a backward insert	Ø		July 21
D21'	bw-insert lox66-DapAsubtilis	lox66-DapAsubtilis P6' PCR product	XbaI	PstI	~960	PCR product digested for cloning as a BI		34	July 30
D22	pSB1A2 open vector	<bbpart>BBa_J61047</bbpart> (MP9.1 & MP9.2)	EcoI	PstI	Ø D22.1	Open pSB1A2 vector for BioBrick EcoRI/PstI insertio	Ø (24/09/07)	D22.2:59	July 23, Aug. 13
D23	AraC/pBad promoter fw-insert	<bbpart>BBa_I0500</bbpart> (MP4.1 & MP4.2)	EcoI	SpeI	1233	AraC/pBad promoter ready for use as a forward insert	Ø D23.1	93&53	July 23
D24	eCFP bw-insert	<bbpart>BBa_E0422</bbpart> (MP6.1 & MP6.2)	XbaI	PstI		eCFP (RBS-OFR_LVA-T) ready for use as a backward insert		56&55	July 23
D26	gfp-tripart	<bbpart>BBa_E0840</bbpart> (MP8.1 & MP8.2)	XbaI	PstI		gfp-tri part (strongRBS-ORF-T) ready for use as a backward insert		47&47	July 23
D27	lox71-ftsZ bw-insert	lox71-ftsZ PCR product P7	XbaI	PstI		lox71-ftsZ(unmutated) ready for use as a backward insert	Ø		July 23
D27'	lox71-ftsZ bw-insert	lox71-ftsZ PCR product P7	XbaI	PstI		lox71-ftsZ(unmutated) ready for use as a backward insert		24	July 30
D28	RBS-DapAColi BB	P8 (RBS-DapAColi)	EcoRI	PstI	~950	Biobrick		54	July 25
D29	RBS-DapAColi	P8 (RBS-DapAColi)	XbaI	PstI	~950	Backward insert		44	July 25
D30	RBS-DapASubtilis BB	P9 (RBS-DapASubtilis)	EcoRI	PstI	~950	Biobrick		72	July 25
D31	RBS-DapASubtilis	P9 (RBS-DapASubtilis)	XbaI	PstI	~950	Backward insert		51	July 25
D32	Lox71-FtsA-FtsZ	P10 Lox71-FtsA-FtsZ	XbaI	PstI	~2600	Backward insert - EcoRI site still presents : we can't use EcoRI		47	July 25
D33	RBS(B0030)CreORF	L6' (clone 1 & 2)	XbaI	PstI		RBS-Cre digestion for use as a BI		29&27	July 30, August 13
D34	lox66	L9' lox66	SpeI	PstI		Biobrick plasmid carrying lox66 digested as a BV		18	July 30
D35	lox71	L10' lox71	SpeI	PstI		Biobrick plasmid carrying lox71 digested as a BV			July 30, August 13
D36	lox71-KmR-lox66	P11 lox71-KmR-lox66	EcoRI	PstI		lox71-KmR-lox66 digested as a biobrick insert			July 30
D37	lox71-gfp(tripart) BI	L12.1 & L12.2 miniprep products (from 30/07 ligation reaction)	XbaI	PstI		lox71_RBS-gfp-double terminator extracted as a backward insert			Aug. 6
D38	BB-insert lox66-DapAE.coli	lox66-DapAE.coli in pTOPO (P5 PCR product)(clones 1+2)	EcoRI	PstI		lox66-dapAE.coli extracted from pTOPO plasmid as a BB insert			Aug. 6
D39'	BI-insert lox66-DapAE.coli	lox66-DapAE.coli in pTOPO (P5 PCR product)(clones 1+2)	XbaI	PstI		lox66-dapAcoli extracted for usage as a backward insert			Aug. 7
D40	BB-insert lox71-KmR-lox66	L25.1 miniprep product (from ligation rxn of 30/07	EcoRI	PstI		lox71-KmR-lox66 construct extracted for clonong as a biobrick			Aug. 6
D41	araC/pBad-J61002 plasmid SP dig.	L1.2, L1".1 & L1".2 miniprep product	SpeI	PstI		J61002 plasmid containing araC/pBad promotor digested for insertion of a BI CDS			Aug. 13
D42	Lox66 FV	L9	EcoRI	XbaI		pSB1A2-lox66 as a Forward vector			August 13
D43	gfp-tripart FI	MP8.1	EcoRI	SpeI		gfp-tri part (strongRBS-ORF-T) ready for use as a forward insert			August 13
D44	<bbpart>B0015</bbpart> terminator BI	<bbpart>B0015</bbpart> in <bbpart>pSB1AK3</bbpart> (MP3.3 Aug.14)	XbaI	Pst1		terminator B0015 extracted for use as a BI			August 16
D45	<bbpart>B0015</bbpart> terminator FI	<bbpart>B0015</bbpart> in <bbpart>pSB1AK3</bbpart> (MP3.4 Aug.14)	EcoRI	SpeI		terminator B0015 extracted for use as a FI			August 16
D46	<bbpart>B0015</bbpart> BV	<bbpart>B0015</bbpart> in <bbpart>pSB1AK3</bbpart> (MP3.3 Aug.14)	SpeI	Pst1		<bbpart>B0015</bbpart> in <bbpart>pSB1AK3</bbpart> plasmid extracted for use as a BV			August 16
D47	<bbpart>pSB1A2</bbpart> XP dig.	<bbpart>pSB1A2</bbpart> carrying Cre ORF (MP9.1)	XbaI	PstI		<bbpart>pSB1A2</bbpart> open vector ready for cloning of BI			August 16
D48	<bbpart>pSB1A2</bbpart> ES dig.	<bbpart>pSB1A2</bbpart> carrying Cre ORF (MP9.2)	EcoRI	SpeI		<bbpart>pSB1A2</bbpart> open vector ready for cloning of FI			August 16
D49	lox71-gfptripart BI	lox71-gfptripart in <bbpart>pSB1A2</bbpart> (L12.2)	XbaI	Pst1		lox71-gfptripart extracted for use as a BI			August 16
D50	<bbpart>BBa_J61002</bbpart> pTet SP dig.	<bbpart>BBa_J61002</bbpart> (MP13.1 Aug. 14)	SpeI	PstI		<bbpart>BBa_J61002</bbpart> pTet promoter vector ready for insertion of CDS as a BI			August 16
D51	RBS-dapAcoli BBinsert	pTOPO of RBS-dapAcoli (MPT4.3)	EcoRI	Pst1		RBS-dapAcoli extracted for cloning as a biobrick			August 16
D52	RBS-dapAcoli BI	pTOPO of RBS-dapAcoli (MPT4.3)	XbaI	Pst1		RBS-dapAcoli extracted for use as a BI			August 16
D53	RBS-dapAsubtilis BBinsert	pTOPO of RBS-dapAsubtilis (MPT5.1)	EcoRI	Pst1		RBS-dapAsubtilis extracted for cloning as a biobrick			August 16
D54	RBS-dapAsubtilis BI	pTOPO of RBS-dapAsubtilis (MPT5.1)	XbaI	Pst1		RBS-dapAsubtilis extracted for use as a BI	Ø (24/09/07)		August 16
D55	RBS-dapAsubtilis FI	pTOPO of RBS-dapAsubtilis (MPT5.2)	EcoRI	SpeI		RBS-dapAsubtilis extracted for use as a FI			August 16
D57	lox71-B0015(T) BI	(L31.1, L31.3) lox71-B0015(T) <bbpart>pSB1A2</bbpart>	XbaI	Pst1		lox71-B0015(T) extracted for use as a BI <bbpart>pSB1A2</bbpart>			Sep. 14
D58	B0015(T)-lox66 BV	(L32.1, L32.2) B0015(T)-lox66 BV	SpeI	PstI		B0015(T)-lox66 for use as a BV <bbpart>pSB1A2</bbpart>			Sep. 14
D59	pTet>>lox71-gfptripart BI	(L34.1, L34.2) pTet>>lox71-gfptripart <bbpart>BBa_J61002</bbpart>	XbaI	PstI		pTet>>lox71-gfptripart for use as a BI			Sep. 14
D61	lox66-mRFP FV	(L36.1, L36.2) lox66-mRFP	EcoRI	XbaI		lox66-mRFP part (strongRBS-ORF-T) ready for use as a forward vector			Sep. 14
D62	lox66-RBSDapAcoli BI	(L37.1, L37.2) lox66-RBSDapAcoli	XbaI	PstI		lox66-RBSDapAcoli ready for use as a Backward insert			Sep. 14
D63	RBSDapAcoli BI	(L39.1, L39.2) RBSDapAcoli	XbaI	PstI		RBSDapAcoli BI (for insertion behind T-lox66)			Sep. 14
D64	FRT-CmR-FRT <bbpart>BBa_J61025</bbpart> BV	(MP14.1) <bbpart>BBa_J61025</bbpart>	SpeI	PstI		FRT-CmR-FRT <bbpart>BBa_J61025</bbpart> (for backward introduction of a casette for Wanner K insertion)			Sep. 14
D65	FRT-CmR-FRT <bbpart>BBa_J61025</bbpart> FV	(MP14.1) <bbpart>BBa_J61025</bbpart>	EcoR	XbaI		FRT-CmR-FRT <bbpart>BBa_J61025</bbpart> (for forward introduction of a casette for Wanner K insertion)			Sep. 14
D68	attB BV	(MP15.1)	SpeI	PstI		vector containing attB for use as a BV			Sep. 14
D69	gfptripart-lox66 BV	(L29.1) gfptripart-lox66 <bbpart>pSB1A2</bbpart>	SpeI	PstI					Sep. 14
D70	pTet>>lox71-gfptripart FI	(L34.1, L34.2) pTet>>lox71-gfptripart <bbpart>BBa_J61002</bbpart>	EcoRI	SpeI		pTet>>lox71-gfptripart for use as a FI			Sep. 14
D71	araC/pBad>>lox71-gfptripart FI	L35.1 araC/pBad>>lox71-gfptripart <bbpart>BBa_J61002</bbpart>	EcoRI	SpeI		araC/pBad>>lox71-gfptripart for use as a FI	Ø (24/09/07)		Sep. 14 Sep. 17
D72	<bbpart>pSB2K3</bbpart> BBvector	MP16 <bbpart>pSB2K3</bbpart>::attP	EcoRI	PstI		Low copy number plasmid, BB vector			Sep. 17
D73	<bbpart>pSB2K3</bbpart> ES open vector	MP16 <bbpart>pSB2K3</bbpart>::attP	EcoRI	SpeI		Comparison of digestion efficiency between Pst and Spe in NEB2			Sep. 17
D76	DGAT BB	P14 DGAT	EcoRI	PstI		DGAT	Ø (24/09/07)		Sep. 17
D77	araC/pBad>>lox71-gfptripart BI	D60 (.1 & .2) digestion product	FspI	AhdI		in D60, the extracted fragment & the open plasmid have the same size, it is thus necessary to remove the plasmid by digestion			Sep. 17
D78	araC/pBad>>lox71-gfptripart FI	D71 (.1 & .2) digestion product	FspI	AhdI		in D71, the extracted fragment & the open plasmid have the same size, it is thus necessary to remove the plasmid by digestion			Sep. 17

@@ Line 48: / Line 48: @@
 |- style="background: #ccccff; text-align: center;"
 |width=5%| Number
-|width=15%| Name
+|width=10%| Name
-|width=5%| Biobricks
+|width=15%| Biobricks
 |width=45%| Description
 |width=10%| Products in use
-|width=20| Date
+|width=20%| Date
 |- style="background: #cccccc;"
 | style="background: #ccffcc;" |MP1
-|align="center"|[[Image:Part_icon_regulatory.png]]
 |align="center"|[http://partsregistry.org/Part:BBa_J23100 pJ23100] in [http://partsregistry.org/Part:BBa_J61002 J61002]
+|align="center"|[[Image:Part_icon_regulatory.png]]
 |align="center"|Strong constitutive promoter in J61002 (clones 1 + 2)
 |
@@ Line 62: / Line 62: @@
 |- style="background: #cccccc;"
 | style="background: #ccffcc;" |MP2
-|align="center"|[[Image:Part_icon_regulatory.png]]
 |align="center"|[http://partsregistry.org/Part:BBa_J23107 pJ23107] in [http://partsregistry.org/Part:BBa_J61002 J61002]
+|align="center"|[[Image:Part_icon_regulatory.png]]
 |align="center"|Medium constitutive promoter in J61002 (clones 1 + 2)
 |align="center"|MP2.2
@@ Line 69: / Line 69: @@
 |- style="background: #cccccc;"
 | style="background: #ccffcc;" |MP3
-|align="center"|[[Image:Part_icon_terminator.png]][[
 |align="center"|[http://partsregistry.org/Part:BBa_B0015 B0015] in [http://partsregistry.org/Part:BBa_SB1AK3 pSB1AK3]
-|align="center"|Double terminator (clones 1 + 2) Image:Part_icon_terminator.png]]
+|align="center"|[[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
+|align="center"|Double terminator (clones 1 + 2)
 |align="center"|MP3.3, MP3.4
 |align="center"|2007
 |- style="background: #cccccc;"
 | style="background: #ccffcc;" |MP4
-|align="center"|[[Image:Part_icon_regulatory.png]]
 |align="center"|[http://partsregistry.org/Part:BBa_I0500 I0500] in [http://partsregistry.org/Part:BBa_SB2K3 pSB2K3]
+|align="center"|[[Image:Part_icon_regulatory.png]]
 |align="center"|AraC pBAD (clones 1 + 2)
 |
@@ Line 83: / Line 83: @@
 |- style="background: #cccccc;"
 | style="background: #ccffcc;" |MP5
-|align="center"|[[Image:Part_icon_rbs.png]]
 |align="center"|[http://partsregistry.org/Part:BBa_B0030 B0030] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2]
+|align="center"|[[Image:Part_icon_rbs.png]]
 |align="center"|Strong RBS (clones 1 + 2)
 |
@@ Line 90: / Line 90: @@
 |- style="background: #cccccc;"
 | style="background: #ccffcc;" |MP6
-|align="center"|[[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]]
 |align="center"|[http://partsregistry.org/Part:BBa_E0422 E0422] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2]
-|align="center"|ECFP (RBS+LVA+Term) (clones 1 + 2) |align="center"|MP6'.1, MP6'.2
+|align="center"|[[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]]
+|align="center"|ECFP (RBS+LVA+Term) (clones 1 + 2)
+|align="center"|MP6'.1, MP6'.2
 |align="center"|2007
 |- style="background: #cccccc;"
 | style="background: #ccffcc;" |MP8
-|align="center"|[[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]]
 |align="center"|[http://partsregistry.org/Part:BBa_E0840 E0840] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2]
+|align="center"|[[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]]
 |align="center"|gfp tri-part; strong rbs (clones 1 + 2)
 |
 |align="center"|2007
 |}
 == Digestion Products ==