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Team:The University of Alberta/21 July 2008
From 2008.igem.org
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*Finished Westerns of the Butanol suff in J6...RESULTS PENDING | *Finished Westerns of the Butanol suff in J6...RESULTS PENDING | ||
**Results in. Didn't work. Again. Either we're doing the Westerns incorrectly or the butanol parts aren't being translated for some reason. | **Results in. Didn't work. Again. Either we're doing the Westerns incorrectly or the butanol parts aren't being translated for some reason. | ||
| + | *Worked out the procedure for putting together all of our parts. | ||
| + | *Re-digesting Lac1 ERE, TetR. RBS.6x His, RBS-6xHis, TDNA-MCS with Xba/Pst. | ||
| + | *Digested BisDA and BisDB with Xba/Pst. Ran them on gel. | ||
| + | **They looked good, so gel purified the bands. | ||
| + | *Cut I0500 with Spe and Pst. No bands appeared on gel. Reran them on gel and got two bands. | ||
| + | **Set up the O/N's using the colonies on plate. Minipreps to be done on Tuesday. | ||
Latest revision as of 16:10, 22 July 2008
Today
- Transformed the ligation of Tryp into J6
- Finished Westerns of the Butanol suff in J6...RESULTS PENDING
- Results in. Didn't work. Again. Either we're doing the Westerns incorrectly or the butanol parts aren't being translated for some reason.
- Worked out the procedure for putting together all of our parts.
- Re-digesting Lac1 ERE, TetR. RBS.6x His, RBS-6xHis, TDNA-MCS with Xba/Pst.
- Digested BisDA and BisDB with Xba/Pst. Ran them on gel.
- They looked good, so gel purified the bands.
- Cut I0500 with Spe and Pst. No bands appeared on gel. Reran them on gel and got two bands.
- Set up the O/N's using the colonies on plate. Minipreps to be done on Tuesday.
